Staff View: A Study on the Presence of Pathogenic Leptospira spp. in Environmental Water Samples Obtained from Selected Recreational Areas in Terengganu, Malaysia

A Study on the Presence of Pathogenic Leptospira spp. in Environmental Water Samples Obtained from Selected Recreational Areas in Terengganu, Malaysia

Leptospirosis is a zoonosis of worldwide distribution. It is endemic in Malaysia and is caused by infection with pathogenic Leptospira species. Leptospires can survive in water for many months hence water is an important vehicle in their transmission. Recreational activities such as swimming, fish...

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Main Authors:	Salwani, Ismail, Nor Iza, A.Rahman, Noor Zarina, Abd Wahab
Format:	Article
Language:	English English
Published:	2014
Subjects:	R Medicine (General)
Online Access:	http://eprints.unisza.edu.my/5653/1/FH02-FP-15-03039.pdf http://eprints.unisza.edu.my/5653/2/FH02-FP-16-05367.jpg http://eprints.unisza.edu.my/5653/
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id	my-unisza-ir.5653
record_format	eprints
spelling	my-unisza-ir.56532022-09-13T05:39:07Z http://eprints.unisza.edu.my/5653/ A Study on the Presence of Pathogenic Leptospira spp. in Environmental Water Samples Obtained from Selected Recreational Areas in Terengganu, Malaysia Salwani, Ismail Nor Iza, A.Rahman Noor Zarina, Abd Wahab R Medicine (General) Leptospirosis is a zoonosis of worldwide distribution. It is endemic in Malaysia and is caused by infection with pathogenic Leptospira species. Leptospires can survive in water for many months hence water is an important vehicle in their transmission. Recreational activities such as swimming, fishing, canoeing and rafting place a person at risk for the infection. The objective of this study was to detect the presence of pathogenic Leptospira spp. in environmental water samples obtained from selected recreational areas in Terengganu. A total of 40 water samples (2000 ml) were collected from two recreational areas in north Terengganu namely Lata Tembakah and Lata Belatan Waterfalls. The samples were inoculated into modified semisolid Ellinghausen McCullough Johnson Harris (EMJH) media, incubated at 30°C for one month and examined under the dark-field microscope. Positive growth for the pathogenic leptospires was then confirmed by Polymerase Chain Reaction (PCR) assay using G1/G2 primers. Pathogenic leptospires were detected in both recreational areas, 5% (1 out of 20) samples and 10% (2 out of 20) samples from Lata Tembakah Waterfall and Lata Belatan Waterfall respectively and were confirmed positive for the presence of pathogenic leptospires. As a conclusion, pathogenic leptospires were detected at two recreational areas in Terengganu and this would pose a risk of infection to human and lead to leptospirosis outbreak. Therefore, based on the result of this study, precautionary steps should be taken by responsible authorities to prevent human infection or leptospirosis outbreak. 2014-10 Article PeerReviewed text en http://eprints.unisza.edu.my/5653/1/FH02-FP-15-03039.pdf image en http://eprints.unisza.edu.my/5653/2/FH02-FP-16-05367.jpg Salwani, Ismail and Nor Iza, A.Rahman and Noor Zarina, Abd Wahab (2014) A Study on the Presence of Pathogenic Leptospira spp. in Environmental Water Samples Obtained from Selected Recreational Areas in Terengganu, Malaysia. Research J. Pharm. and Tech, 7 (10). pp. 1153-1157. ISSN 0974-3618
institution	Universiti Sultan Zainal Abidin
building	UNISZA Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Sultan Zainal Abidin
content_source	UNISZA Institutional Repository
url_provider	https://eprints.unisza.edu.my/
language	English English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Salwani, Ismail Nor Iza, A.Rahman Noor Zarina, Abd Wahab A Study on the Presence of Pathogenic Leptospira spp. in Environmental Water Samples Obtained from Selected Recreational Areas in Terengganu, Malaysia
description	Leptospirosis is a zoonosis of worldwide distribution. It is endemic in Malaysia and is caused by infection with pathogenic Leptospira species. Leptospires can survive in water for many months hence water is an important vehicle in their transmission. Recreational activities such as swimming, fishing, canoeing and rafting place a person at risk for the infection. The objective of this study was to detect the presence of pathogenic Leptospira spp. in environmental water samples obtained from selected recreational areas in Terengganu. A total of 40 water samples (2000 ml) were collected from two recreational areas in north Terengganu namely Lata Tembakah and Lata Belatan Waterfalls. The samples were inoculated into modified semisolid Ellinghausen McCullough Johnson Harris (EMJH) media, incubated at 30°C for one month and examined under the dark-field microscope. Positive growth for the pathogenic leptospires was then confirmed by Polymerase Chain Reaction (PCR) assay using G1/G2 primers. Pathogenic leptospires were detected in both recreational areas, 5% (1 out of 20) samples and 10% (2 out of 20) samples from Lata Tembakah Waterfall and Lata Belatan Waterfall respectively and were confirmed positive for the presence of pathogenic leptospires. As a conclusion, pathogenic leptospires were detected at two recreational areas in Terengganu and this would pose a risk of infection to human and lead to leptospirosis outbreak. Therefore, based on the result of this study, precautionary steps should be taken by responsible authorities to prevent human infection or leptospirosis outbreak.
format	Article
author	Salwani, Ismail Nor Iza, A.Rahman Noor Zarina, Abd Wahab
author_facet	Salwani, Ismail Nor Iza, A.Rahman Noor Zarina, Abd Wahab
author_sort	Salwani, Ismail
title	A Study on the Presence of Pathogenic Leptospira spp. in Environmental Water Samples Obtained from Selected Recreational Areas in Terengganu, Malaysia
title_short	A Study on the Presence of Pathogenic Leptospira spp. in Environmental Water Samples Obtained from Selected Recreational Areas in Terengganu, Malaysia
title_full	A Study on the Presence of Pathogenic Leptospira spp. in Environmental Water Samples Obtained from Selected Recreational Areas in Terengganu, Malaysia
title_fullStr	A Study on the Presence of Pathogenic Leptospira spp. in Environmental Water Samples Obtained from Selected Recreational Areas in Terengganu, Malaysia
title_full_unstemmed	A Study on the Presence of Pathogenic Leptospira spp. in Environmental Water Samples Obtained from Selected Recreational Areas in Terengganu, Malaysia
title_sort	study on the presence of pathogenic leptospira spp. in environmental water samples obtained from selected recreational areas in terengganu, malaysia
publishDate	2014
url	http://eprints.unisza.edu.my/5653/1/FH02-FP-15-03039.pdf http://eprints.unisza.edu.my/5653/2/FH02-FP-16-05367.jpg http://eprints.unisza.edu.my/5653/
_version_	1744358534257049600
score	13.211869

A Study on the Presence of Pathogenic Leptospira spp. in Environmental Water Samples Obtained from Selected Recreational Areas in Terengganu, Malaysia

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