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Zamzam Water: Influence of Containers on Ionic Concentration and In-vitro Cytotoxic Effects on U87 Cell Line

Zamzam is holy water believed by Muslim to have remedial power for all kinds of diseases. It contains many electrolytes and the concentration of the electrolytes may be affected by the types of container used for its storage. This study was carried out to determine the difference in ions concentrati...

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Main Authors: Mohd Ekhwan, Toriman, Nasir, Mohamad, Ahmad Zubaidi, A.latif, Nor Hidayah, Abu Bakar
Format: Article
Language:English
Published: 2016
Subjects:
R Medicine (General)
Online Access:http://eprints.unisza.edu.my/5349/1/FH02-FP-17-08354.pdf
http://eprints.unisza.edu.my/5349/
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spelling my-unisza-ir.53492022-02-13T03:27:07Z http://eprints.unisza.edu.my/5349/ Zamzam Water: Influence of Containers on Ionic Concentration and In-vitro Cytotoxic Effects on U87 Cell Line Mohd Ekhwan, Toriman Nasir, Mohamad Ahmad Zubaidi, A.latif Nor Hidayah, Abu Bakar R Medicine (General) Zamzam is holy water believed by Muslim to have remedial power for all kinds of diseases. It contains many electrolytes and the concentration of the electrolytes may be affected by the types of container used for its storage. This study was carried out to determine the difference in ions concentration of Zamzam water stored in plastic and glass containers, and to determine cytotoxicity effects of Zamzam water against U-87 cell line (human primary glioblastoma cell line). Ion Chromatography (IC) was used to analyze the concentration. The analyzed anions in the Zamzam water include bromide, chloride, phosphate, nitrite, nitrate, sulfate and fluoride whereas the cations were ammonium, lithium, potassium, sodium, calcium and magnesium. Subsequently, MTT assay was used to determine the cytotoxicity of Zamzam water on U-87 cell line. This study reveals that Zamzam water anions and cations concentration was not statistically significant neither in plastic nor glass container. In addition, the Zamzam water did not cause any toxicity on the U87 cell line. We postulate that types of container do not have much influence on the ion concentration of Zamzam water and it is non-toxic on U87 cell line. 2016-12 Article PeerReviewed text en http://eprints.unisza.edu.my/5349/1/FH02-FP-17-08354.pdf Mohd Ekhwan, Toriman and Nasir, Mohamad and Ahmad Zubaidi, A.latif and Nor Hidayah, Abu Bakar (2016) Zamzam Water: Influence of Containers on Ionic Concentration and In-vitro Cytotoxic Effects on U87 Cell Line. Malaysian Journal of Applied Sciences, 1 (1). pp. 68-72. ISSN 0127-9246
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Mohd Ekhwan, Toriman
Nasir, Mohamad
Ahmad Zubaidi, A.latif
Nor Hidayah, Abu Bakar
Zamzam Water: Influence of Containers on Ionic Concentration and In-vitro Cytotoxic Effects on U87 Cell Line
description Zamzam is holy water believed by Muslim to have remedial power for all kinds of diseases. It contains many electrolytes and the concentration of the electrolytes may be affected by the types of container used for its storage. This study was carried out to determine the difference in ions concentration of Zamzam water stored in plastic and glass containers, and to determine cytotoxicity effects of Zamzam water against U-87 cell line (human primary glioblastoma cell line). Ion Chromatography (IC) was used to analyze the concentration. The analyzed anions in the Zamzam water include bromide, chloride, phosphate, nitrite, nitrate, sulfate and fluoride whereas the cations were ammonium, lithium, potassium, sodium, calcium and magnesium. Subsequently, MTT assay was used to determine the cytotoxicity of Zamzam water on U-87 cell line. This study reveals that Zamzam water anions and cations concentration was not statistically significant neither in plastic nor glass container. In addition, the Zamzam water did not cause any toxicity on the U87 cell line. We postulate that types of container do not have much influence on the ion concentration of Zamzam water and it is non-toxic on U87 cell line.
format Article
author Mohd Ekhwan, Toriman
Nasir, Mohamad
Ahmad Zubaidi, A.latif
Nor Hidayah, Abu Bakar
author_facet Mohd Ekhwan, Toriman
Nasir, Mohamad
Ahmad Zubaidi, A.latif
Nor Hidayah, Abu Bakar
author_sort Mohd Ekhwan, Toriman
title Zamzam Water: Influence of Containers on Ionic Concentration and In-vitro Cytotoxic Effects on U87 Cell Line
title_short Zamzam Water: Influence of Containers on Ionic Concentration and In-vitro Cytotoxic Effects on U87 Cell Line
title_full Zamzam Water: Influence of Containers on Ionic Concentration and In-vitro Cytotoxic Effects on U87 Cell Line
title_fullStr Zamzam Water: Influence of Containers on Ionic Concentration and In-vitro Cytotoxic Effects on U87 Cell Line
title_full_unstemmed Zamzam Water: Influence of Containers on Ionic Concentration and In-vitro Cytotoxic Effects on U87 Cell Line
title_sort zamzam water: influence of containers on ionic concentration and in-vitro cytotoxic effects on u87 cell line
publishDate 2016
url http://eprints.unisza.edu.my/5349/1/FH02-FP-17-08354.pdf
http://eprints.unisza.edu.my/5349/
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score 13.211869

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