Chemopreventive potential of methanol extract of Dicranopteris linearis leaf on DMBA/croton oil-induced mouse skin carcinogenesis
The present study was carried out to elucidate the chemopreventive potential of methanol extract of Dicranopteris linearis (MEDL) in a two-stage mouse skin carcinogenesis model due to the interrelated inflammation, oxidative stress and tumor promotion pathways. MEDL was prepared in a dose range...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/4675/1/FH02-FBIM-16-06789.pdf http://eprints.unisza.edu.my/4675/ |
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| Summary: | The present study was carried out to elucidate the chemopreventive potential of methanol extract of
Dicranopteris linearis (MEDL) in a two-stage mouse skin carcinogenesis model due to the interrelated
inflammation, oxidative stress and tumor promotion pathways. MEDL was prepared in a dose range of
30 to 300 mg/kg body weight. A total of 48 imprinting control region (ICR) female mice (6 to 8 weeks old)
were randomly assorted into six groups. To induce skin tumor formation, a single topical application of
7,12-dimethylbenz[a]anthracene (DMBA) at 100 μg/100 μl was applied to the shaved dorsal region of
mice, followed by repetitive administration of 1% croton oil, twice weekly for 15 weeks. Topical
application of MEDL, 30 min prior to the croton oil application significantly reduced the tumor incidence
to 12.5% in 300 mg/kg MEDL-treated group as compared to 87.5% in carcinogen control. The latency
period of tumor formation was increased from sixth week in the carcinogen control to ninth and fifteenth
weeks in 100 and 300 mg/kg MEDL-treated groups, respectively. The tumor burden of MEDL-treated
groups (30, 100, and 300 mg/kg) were significantly lessen (5.67 ± 1.28, 5.00 ± 1.13, and 1.00 ± 0.13), as
compared to carcinogen control (7.86 ± 2.37). The tumor volume was also significantly reduced from
9.00 ± 2.27 mm3
in carcinogen control to 3.70 ± 0.96, 2.39 ± 0.54 and 0.26 ± 0.03 mm3
in 30, 100 and 300
mg/kg MEDL-treated groups, respectively. In conclusion, the MEDL exhibited anti-carcinogenic effect in
a dose-dependent manner, indicating its chemopreventive potential, which worth further study. |
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