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Detection, antibiogram and molecular characterization of MRSA and MSSA isolated from swine

The emergence of bacteria with antimicrobial resistance traits such as methicillin-resistant Staphylococcus aureus (MRSA) in food-producing animals is a significant public health concern. The aim of this study is to determine the prevalence rate, antibiotic susceptibility and the molecular charac...

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Main Authors: Mohd Faizal, Ghazali, Noor Muzamil, Mohamad, Siti Mariam, Zainal Ariffin
Format: Conference or Workshop Item
Language:English
English
Published: 2021
Subjects:
Q Science (General)
QH426 Genetics
QR Microbiology
Online Access:http://eprints.unisza.edu.my/4351/1/FH03-FBIM-21-54495.pdf
http://eprints.unisza.edu.my/4351/2/FH03-FBIM-21-56902.pdf
http://eprints.unisza.edu.my/4351/
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spelling my-unisza-ir.43512022-01-04T04:06:54Z http://eprints.unisza.edu.my/4351/ Detection, antibiogram and molecular characterization of MRSA and MSSA isolated from swine Mohd Faizal, Ghazali Noor Muzamil, Mohamad Siti Mariam, Zainal Ariffin Q Science (General) QH426 Genetics QR Microbiology The emergence of bacteria with antimicrobial resistance traits such as methicillin-resistant Staphylococcus aureus (MRSA) in food-producing animals is a significant public health concern. The aim of this study is to determine the prevalence rate, antibiotic susceptibility and the molecular characteristics of Staphylococcus aureus (S. aureus) in swine from selected swine farms in Peninsular Malaysia. The oral and nasal swabs of 200 live swine were collected. Screening and isolation of S. aureus isolates were carried out using phenotypic identification techniques. The identity of the bacteria isolates was confirmed by detection of nuc gene (S. aureus) and mecA gene (MRSA). Antibiogram of S. aureus against 19 antibiotics was developed using Kirby-Bauer test. Molecular detections of antimicrobial resistance and virulence genes as well spa typing of S. aureus was carried out. 25% of swine were carrying S. aureus with 9% being MRSA carriers. S. aureus and MRSA have shown high resistance against clindamycin, tetracycline, chloramphenicol, oxacillin, penicillin, erythromycin, cefoxitin and amoxicillin/clavulanate. Antimicrobial resistance and virulence genes, including tetK, tetL, tetM, ermA, ermB, ermC, msrA and scn genes were detected. 93% of S. aureus were multiple-drug resistant (MDR), suggesting the emergence of MDR S. aureus in swine in Malaysia. 2021 Conference or Workshop Item PeerReviewed text en http://eprints.unisza.edu.my/4351/1/FH03-FBIM-21-54495.pdf text en http://eprints.unisza.edu.my/4351/2/FH03-FBIM-21-56902.pdf Mohd Faizal, Ghazali and Noor Muzamil, Mohamad and Siti Mariam, Zainal Ariffin (2021) Detection, antibiogram and molecular characterization of MRSA and MSSA isolated from swine. In: 2nd International Conference on Animal Production for Food Sustainability, 16 Jun 2021, Bali, Indonesia.
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
English
topic Q Science (General)
QH426 Genetics
QR Microbiology
spellingShingle Q Science (General)
QH426 Genetics
QR Microbiology
Mohd Faizal, Ghazali
Noor Muzamil, Mohamad
Siti Mariam, Zainal Ariffin
Detection, antibiogram and molecular characterization of MRSA and MSSA isolated from swine
description The emergence of bacteria with antimicrobial resistance traits such as methicillin-resistant Staphylococcus aureus (MRSA) in food-producing animals is a significant public health concern. The aim of this study is to determine the prevalence rate, antibiotic susceptibility and the molecular characteristics of Staphylococcus aureus (S. aureus) in swine from selected swine farms in Peninsular Malaysia. The oral and nasal swabs of 200 live swine were collected. Screening and isolation of S. aureus isolates were carried out using phenotypic identification techniques. The identity of the bacteria isolates was confirmed by detection of nuc gene (S. aureus) and mecA gene (MRSA). Antibiogram of S. aureus against 19 antibiotics was developed using Kirby-Bauer test. Molecular detections of antimicrobial resistance and virulence genes as well spa typing of S. aureus was carried out. 25% of swine were carrying S. aureus with 9% being MRSA carriers. S. aureus and MRSA have shown high resistance against clindamycin, tetracycline, chloramphenicol, oxacillin, penicillin, erythromycin, cefoxitin and amoxicillin/clavulanate. Antimicrobial resistance and virulence genes, including tetK, tetL, tetM, ermA, ermB, ermC, msrA and scn genes were detected. 93% of S. aureus were multiple-drug resistant (MDR), suggesting the emergence of MDR S. aureus in swine in Malaysia.
format Conference or Workshop Item
author Mohd Faizal, Ghazali
Noor Muzamil, Mohamad
Siti Mariam, Zainal Ariffin
author_facet Mohd Faizal, Ghazali
Noor Muzamil, Mohamad
Siti Mariam, Zainal Ariffin
author_sort Mohd Faizal, Ghazali
title Detection, antibiogram and molecular characterization of MRSA and MSSA isolated from swine
title_short Detection, antibiogram and molecular characterization of MRSA and MSSA isolated from swine
title_full Detection, antibiogram and molecular characterization of MRSA and MSSA isolated from swine
title_fullStr Detection, antibiogram and molecular characterization of MRSA and MSSA isolated from swine
title_full_unstemmed Detection, antibiogram and molecular characterization of MRSA and MSSA isolated from swine
title_sort detection, antibiogram and molecular characterization of mrsa and mssa isolated from swine
publishDate 2021
url http://eprints.unisza.edu.my/4351/1/FH03-FBIM-21-54495.pdf
http://eprints.unisza.edu.my/4351/2/FH03-FBIM-21-56902.pdf
http://eprints.unisza.edu.my/4351/
_version_ 1724079328840384512
score 13.211869

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