Molecular detection and antibiogram of staphylococcus aureus and MRSA from recreational beaches and river sites in Terengganu, Malaysia.
Background: The emergence of antimicrobial resistance (AMR) in Staphylococcus aureus such as methicillin-resistant S. aureus (MRSA) is an expanding public health and veterinary concern. S. aureus remains ubiquitous in the environment and normal flora of animals; hence, the presence of MRSA could...
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| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2021
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/4349/1/FH03-FBIM-21-54497.pdf http://eprints.unisza.edu.my/4349/ |
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| Summary: | Background: The emergence of antimicrobial resistance (AMR) in Staphylococcus aureus such
as methicillin-resistant S. aureus (MRSA) is an expanding public health and veterinary concern. S.
aureus remains ubiquitous in the environment and normal flora of animals; hence, the presence of
MRSA could result in significant morbidity and mortality towards human. Aside from medical and
community settings, both S. aureus and MRSA have previously been reported in sand and seawater
from recreational beaches, suggesting the wide dissemination of AMR strains in these environments.
This aims of this study was to investigate the prevalence and determine the antibiotic susceptibility
profile of S. aureus from selected recreational beaches and river sites in Terengganu, Malaysia.
Methodology: A total of 120 samples (60 sand and 60 water samples) were collected from 10
different sampling sites (4 beaches and 6 river sites). Both sand and water samples were enriched
in tryptone soy broth for 24 hours. The samples were sub-cultured on Mannitol Salt Agar and nutrient
agar for further bacteria amplification and purification. The identities of the suspected S. aureus were
determined using species specific nuc (S. aureus) and mecA (MRSA) genes primers. The
antibiogram profile of the S. aureus isolates against 19 antibiotics was determined via Kirby-Bauer
disk method.
Results: A total 27 S. aureus (22.5%; 27/120) were isolated from 3 beaches and 2 river sites. PCR
revealed the presence of one MRSA (0.8%; 1/120). The isolated S. aureus were found showing
high resistance rate against penicillin (85.2%), amoxicillin/clavulanate (62.9%) and oxacillin (62.9%).
44% of the isolates can be categorised as multidrug-resistant S. aureus (MDRSA).
Conclusion: This study demonstrated the presence of S. aureus, MDRSA and MRSA in
recreational beaches and river sites in Terengganu, Malaysia. |
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