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Betulinic acid inhibits growth of cultured vascular smooth muscle cells in vitro by inducing G1 arrest and apoptosis

Betulinic acid inhibits growth of cultured vascular smooth muscle cells in vitro by inducing G1 arrest and apoptosis (Article) Vadivelu, R.K.a u, Yeap, S.K.a g2, Ali, A.M.b erd, Hamid, M.c u, Alitheen, N.B.d it a Institute of Bioscience, Universiti Putra Malaysia, Selangor, Serdang, Malaysia b Fac...

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Main Authors:	Abd Manaf, Ali, Raja Kumar, Vadivelu, Swee, Keong Yeap, Muhajir, Hamid
Format:	Article
Language:	English English
Published:	2012
Subjects:	R Medicine (General)
Online Access:	http://eprints.unisza.edu.my/2444/1/FH02-FPBSM-14-00670.pdf http://eprints.unisza.edu.my/2444/2/FH02-FPBSM-14-01117.jpg http://eprints.unisza.edu.my/2444/
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spelling	my-unisza-ir.24442021-08-21T08:21:21Z http://eprints.unisza.edu.my/2444/ Betulinic acid inhibits growth of cultured vascular smooth muscle cells in vitro by inducing G1 arrest and apoptosis Abd Manaf, Ali Raja Kumar, Vadivelu Swee, Keong Yeap Muhajir, Hamid R Medicine (General) Betulinic acid inhibits growth of cultured vascular smooth muscle cells in vitro by inducing G1 arrest and apoptosis (Article) Vadivelu, R.K.a u, Yeap, S.K.a g2, Ali, A.M.b erd, Hamid, M.c u, Alitheen, N.B.d it a Institute of Bioscience, Universiti Putra Malaysia, Selangor, Serdang, Malaysia b Faculty of Agriculture and Biotechnology, Universiti Sultan Zainal Abidin, Kampus Kota, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Malaysia Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, 43400 Serdang, Malaysia View additional affiliations Abstract V View references (30) Betulinic acid is a widely available plant-derived triterpene which is reported to possess selective cytotoxic activity against cancer cells of neuroectodermal origin and leukemia. However, the potential of betulinic acid as an antiproliferative and cytotoxic agent on vascular smooth muscle (VSMC) is still unclear. This study was carried out to demonstrate the antiproliferative and cytotoxic effect of betulinic acid on VSMCs using 3-[4,5-dimethylthizol-2- y11-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry cell cycle assay, BrdU proliferation assay, acridine orange/propidium iodide staining, and comet assay. Result from MTT and BrdU assays indicated that betulinic acid was able to inhibit the growth and proliferation of VSMCs in a dose-dependent manner with IC50 of 18 g/mL significantly (13<0.05). Nevertheless, betulinic acid exhibited G1 cell cycle arrest in flow cytometry cell cycle profiling and low level of DNA damage against VSMC in acridine orange/propidium iodide and comet assay after 24 h of treatment. In conclusion, betulinic acid induced Gi cell cycle arrest and dose-dependent DNA damage on VSMC. 2012 Raja Kumar Vadivelu et al. Reaxys Database Information 2012 Article NonPeerReviewed text en http://eprints.unisza.edu.my/2444/1/FH02-FPBSM-14-00670.pdf image en http://eprints.unisza.edu.my/2444/2/FH02-FPBSM-14-01117.jpg Abd Manaf, Ali and Raja Kumar, Vadivelu and Swee, Keong Yeap and Muhajir, Hamid (2012) Betulinic acid inhibits growth of cultured vascular smooth muscle cells in vitro by inducing G1 arrest and apoptosis. Evidence-based complementary and alternative medicine. ISSN 1741427X
institution	Universiti Sultan Zainal Abidin
building	UNISZA Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Sultan Zainal Abidin
content_source	UNISZA Institutional Repository
url_provider	https://eprints.unisza.edu.my/
language	English English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Abd Manaf, Ali Raja Kumar, Vadivelu Swee, Keong Yeap Muhajir, Hamid Betulinic acid inhibits growth of cultured vascular smooth muscle cells in vitro by inducing G1 arrest and apoptosis
description	Betulinic acid inhibits growth of cultured vascular smooth muscle cells in vitro by inducing G1 arrest and apoptosis (Article) Vadivelu, R.K.a u, Yeap, S.K.a g2, Ali, A.M.b erd, Hamid, M.c u, Alitheen, N.B.d it a Institute of Bioscience, Universiti Putra Malaysia, Selangor, Serdang, Malaysia b Faculty of Agriculture and Biotechnology, Universiti Sultan Zainal Abidin, Kampus Kota, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Malaysia Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, 43400 Serdang, Malaysia View additional affiliations Abstract V View references (30) Betulinic acid is a widely available plant-derived triterpene which is reported to possess selective cytotoxic activity against cancer cells of neuroectodermal origin and leukemia. However, the potential of betulinic acid as an antiproliferative and cytotoxic agent on vascular smooth muscle (VSMC) is still unclear. This study was carried out to demonstrate the antiproliferative and cytotoxic effect of betulinic acid on VSMCs using 3-[4,5-dimethylthizol-2- y11-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry cell cycle assay, BrdU proliferation assay, acridine orange/propidium iodide staining, and comet assay. Result from MTT and BrdU assays indicated that betulinic acid was able to inhibit the growth and proliferation of VSMCs in a dose-dependent manner with IC50 of 18 g/mL significantly (13<0.05). Nevertheless, betulinic acid exhibited G1 cell cycle arrest in flow cytometry cell cycle profiling and low level of DNA damage against VSMC in acridine orange/propidium iodide and comet assay after 24 h of treatment. In conclusion, betulinic acid induced Gi cell cycle arrest and dose-dependent DNA damage on VSMC. 2012 Raja Kumar Vadivelu et al. Reaxys Database Information
format	Article
author	Abd Manaf, Ali Raja Kumar, Vadivelu Swee, Keong Yeap Muhajir, Hamid
author_facet	Abd Manaf, Ali Raja Kumar, Vadivelu Swee, Keong Yeap Muhajir, Hamid
author_sort	Abd Manaf, Ali
title	Betulinic acid inhibits growth of cultured vascular smooth muscle cells in vitro by inducing G1 arrest and apoptosis
title_short	Betulinic acid inhibits growth of cultured vascular smooth muscle cells in vitro by inducing G1 arrest and apoptosis
title_full	Betulinic acid inhibits growth of cultured vascular smooth muscle cells in vitro by inducing G1 arrest and apoptosis
title_fullStr	Betulinic acid inhibits growth of cultured vascular smooth muscle cells in vitro by inducing G1 arrest and apoptosis
title_full_unstemmed	Betulinic acid inhibits growth of cultured vascular smooth muscle cells in vitro by inducing G1 arrest and apoptosis
title_sort	betulinic acid inhibits growth of cultured vascular smooth muscle cells in vitro by inducing g1 arrest and apoptosis
publishDate	2012
url	http://eprints.unisza.edu.my/2444/1/FH02-FPBSM-14-00670.pdf http://eprints.unisza.edu.my/2444/2/FH02-FPBSM-14-01117.jpg http://eprints.unisza.edu.my/2444/
_version_	1709673160014561280
score	13.211869

Betulinic acid inhibits growth of cultured vascular smooth muscle cells in vitro by inducing G1 arrest and apoptosis

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