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An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of Newcastle disease virus

The phosphoprotein (P) of paramyxoviruses plays a central role in the viral genome replication and transcription The P either binds to the unassembled nucleocapsid (NPO) to assist in viral genomic replication or binds to assembled NP (NP-RNA or NPNC) to transcribe genome to produce the subgenomic mR...

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Main Authors: Abd Manaf, Ali, Raus, R.A, Siang, T.W, Yusoff, K.
Format: Article
Language:English
Published: 2011
Subjects:
Q Science (General)
Online Access:http://eprints.unisza.edu.my/2163/1/FH02-FPBSM-14-00458.png
http://eprints.unisza.edu.my/2163/
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spelling my-unisza-ir.21632021-08-15T03:26:53Z http://eprints.unisza.edu.my/2163/ An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of Newcastle disease virus Abd Manaf, Ali Raus, R.A Siang, T.W Yusoff, K. Q Science (General) The phosphoprotein (P) of paramyxoviruses plays a central role in the viral genome replication and transcription The P either binds to the unassembled nucleocapsid (NPO) to assist in viral genomic replication or binds to assembled NP (NP-RNA or NPNC) to transcribe genome to produce the subgenomic mRNAs In this study the region of P that binds to NPNC was mapped To determine the binding region 18 N- and C-terminally truncated P mutants were synthesized by in vitro translation in rabbit reticulocytes and mixed with purified NP (NPNC) The mutants which did not bind to NP were considered as mutants and they contain deleted regions that may be involved in binding to NP To identify the mutants that did not bind to NP radioimmunoprecipitation and protein binding assays were used Based on radioimmunoprecipitation analysis. it was shown that the region of P that binds to NP is located within the internal region of C-terminal half of P from amino acids 243 to 279 In agreement with the radioimmunoprecipitation analysis. protein binding assay showed that the interactive domain was mapped to the internal region of the C-terminal half of P. However_ a slightly bigger region of interactive domain (amino acids 224 to 279) was determined by the latter assay. As protein binding assay is considered as a more sensitive assay as compared to radioimmunoprecipitation assay_ thus the interactive region of P to NP Fic was located within C-terminal half of P between amino acids 224 to 279. ©2011 Academic Journals 2011 Article NonPeerReviewed image en http://eprints.unisza.edu.my/2163/1/FH02-FPBSM-14-00458.png Abd Manaf, Ali and Raus, R.A and Siang, T.W and Yusoff, K. (2011) An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of Newcastle disease virus. African Journal of Biotechnology. pp. 18882-18891. ISSN 16845315
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Abd Manaf, Ali
Raus, R.A
Siang, T.W
Yusoff, K.
An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of Newcastle disease virus
description The phosphoprotein (P) of paramyxoviruses plays a central role in the viral genome replication and transcription The P either binds to the unassembled nucleocapsid (NPO) to assist in viral genomic replication or binds to assembled NP (NP-RNA or NPNC) to transcribe genome to produce the subgenomic mRNAs In this study the region of P that binds to NPNC was mapped To determine the binding region 18 N- and C-terminally truncated P mutants were synthesized by in vitro translation in rabbit reticulocytes and mixed with purified NP (NPNC) The mutants which did not bind to NP were considered as mutants and they contain deleted regions that may be involved in binding to NP To identify the mutants that did not bind to NP radioimmunoprecipitation and protein binding assays were used Based on radioimmunoprecipitation analysis. it was shown that the region of P that binds to NP is located within the internal region of C-terminal half of P from amino acids 243 to 279 In agreement with the radioimmunoprecipitation analysis. protein binding assay showed that the interactive domain was mapped to the internal region of the C-terminal half of P. However_ a slightly bigger region of interactive domain (amino acids 224 to 279) was determined by the latter assay. As protein binding assay is considered as a more sensitive assay as compared to radioimmunoprecipitation assay_ thus the interactive region of P to NP Fic was located within C-terminal half of P between amino acids 224 to 279. ©2011 Academic Journals
format Article
author Abd Manaf, Ali
Raus, R.A
Siang, T.W
Yusoff, K.
author_facet Abd Manaf, Ali
Raus, R.A
Siang, T.W
Yusoff, K.
author_sort Abd Manaf, Ali
title An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of Newcastle disease virus
title_short An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of Newcastle disease virus
title_full An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of Newcastle disease virus
title_fullStr An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of Newcastle disease virus
title_full_unstemmed An intermediate region in C-terminal of phosphoprotein is required for binding to nucleocapsid of Newcastle disease virus
title_sort intermediate region in c-terminal of phosphoprotein is required for binding to nucleocapsid of newcastle disease virus
publishDate 2011
url http://eprints.unisza.edu.my/2163/1/FH02-FPBSM-14-00458.png
http://eprints.unisza.edu.my/2163/
_version_ 1709673145803210752
score 13.211869

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