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Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis

Telomeraseisribonucleoproteinenzymecomplexconsistingoftwomaincorecomponents;reversetranscriptase(TERT),andRNAtemplatefortelomericDNAsynthesis(TERC).TERTgeneactsascatalyticcomponentinthisenzymeandislocatedonchromosome5p15.IthasbeenreportedthatthereisanamplificationorincreaseofTERTgenecopynumberinhuma...

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Main Authors: Ahmad Syibli, Othman, Nor Atifah, Mohd Adam, Ravindran, Ankathil, Rosline, Hassan
Format: Article
Language:English
Published: 2011
Subjects:
Q Science (General)
Online Access:http://eprints.unisza.edu.my/2053/1/FH02-FSK-19-25054.pdf
http://eprints.unisza.edu.my/2053/
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spelling my-unisza-ir.20532021-08-15T02:19:09Z http://eprints.unisza.edu.my/2053/ Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis Ahmad Syibli, Othman Nor Atifah, Mohd Adam Ravindran, Ankathil Rosline, Hassan Q Science (General) Telomeraseisribonucleoproteinenzymecomplexconsistingoftwomaincorecomponents;reversetranscriptase(TERT),andRNAtemplatefortelomericDNAsynthesis(TERC).TERTgeneactsascatalyticcomponentinthisenzymeandislocatedonchromosome5p15.IthasbeenreportedthatthereisanamplificationorincreaseofTERTgenecopynumberinhumancancercelllinesandprimarytumoursampleswhichmayassociatewithincreasedtelomeraseactivity.Inourstudy,dual-colorassayprobewasusedinfluorescencein situhybridization(FISH)analysisthatcoveredthegenomicregionencodingTERT(Redsignal)togetherwithaspecificsequenceofEGR1(Greensignal)at5q31tofacilitatechromosomeidentification.Innormalcells,theFISHsignalpatternwasobservedastwogreensignalsandtworedsignals(2G2R).AmplificationoftheTERTgenehasbeendetectedinmorethan90%ofthecellsincelllineintheleukaemia.Besidesthat,aneusomyofchromosome5wasalsodetectedindicatingthepresenceofthreecopiesofchromosome5with3G3Rsignalpattern.Thus,theapplicationofinterphaseFISHtechniquefordetectionofTERTgeneamplificationhasbeensuccessfullyoptimized 2011-01 Article NonPeerReviewed text en http://eprints.unisza.edu.my/2053/1/FH02-FSK-19-25054.pdf Ahmad Syibli, Othman and Nor Atifah, Mohd Adam and Ravindran, Ankathil and Rosline, Hassan (2011) Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis. Malaysian journal of biochemistry and molecular biology, 19 (1). pp. 12-15. ISSN 1511-2616
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Ahmad Syibli, Othman
Nor Atifah, Mohd Adam
Ravindran, Ankathil
Rosline, Hassan
Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
description Telomeraseisribonucleoproteinenzymecomplexconsistingoftwomaincorecomponents;reversetranscriptase(TERT),andRNAtemplatefortelomericDNAsynthesis(TERC).TERTgeneactsascatalyticcomponentinthisenzymeandislocatedonchromosome5p15.IthasbeenreportedthatthereisanamplificationorincreaseofTERTgenecopynumberinhumancancercelllinesandprimarytumoursampleswhichmayassociatewithincreasedtelomeraseactivity.Inourstudy,dual-colorassayprobewasusedinfluorescencein situhybridization(FISH)analysisthatcoveredthegenomicregionencodingTERT(Redsignal)togetherwithaspecificsequenceofEGR1(Greensignal)at5q31tofacilitatechromosomeidentification.Innormalcells,theFISHsignalpatternwasobservedastwogreensignalsandtworedsignals(2G2R).AmplificationoftheTERTgenehasbeendetectedinmorethan90%ofthecellsincelllineintheleukaemia.Besidesthat,aneusomyofchromosome5wasalsodetectedindicatingthepresenceofthreecopiesofchromosome5with3G3Rsignalpattern.Thus,theapplicationofinterphaseFISHtechniquefordetectionofTERTgeneamplificationhasbeensuccessfullyoptimized
format Article
author Ahmad Syibli, Othman
Nor Atifah, Mohd Adam
Ravindran, Ankathil
Rosline, Hassan
author_facet Ahmad Syibli, Othman
Nor Atifah, Mohd Adam
Ravindran, Ankathil
Rosline, Hassan
author_sort Ahmad Syibli, Othman
title Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
title_short Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
title_full Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
title_fullStr Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
title_full_unstemmed Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis
title_sort copy number status of tert gene in a myeloid leukaemia cell line, k562: application of interphase fluorescence in situ hybridization (fish) analysis
publishDate 2011
url http://eprints.unisza.edu.my/2053/1/FH02-FSK-19-25054.pdf
http://eprints.unisza.edu.my/2053/
_version_ 1709673135999025152
score 13.211869

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