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Styrylpyrone derivative of goniothalamus umbrosus: new potential in antiviral activity

This study aimed to investigate the potential effect of styrylpyrone derivative (SPD) extracted from Goniothalamus umbrosus root against in vitro dengue virus type 2 (DENV-2) replication. In the present study. cytotoxicity of SPD was evaluated using a cell viability assay. Antiviral activity of SPD,...

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Main Authors: Noor Zarina, Abd Wahab, Norefrina Shafinaz, Md Nor, Nazlina, Ibrahim
Format: Conference or Workshop Item
Language:English
Published: 2019
Subjects:
QR Microbiology
Online Access:http://eprints.unisza.edu.my/1894/1/FH03-FSK-20-36479.pdf
http://eprints.unisza.edu.my/1894/
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spelling my-unisza-ir.18942020-11-24T02:50:45Z http://eprints.unisza.edu.my/1894/ Styrylpyrone derivative of goniothalamus umbrosus: new potential in antiviral activity Noor Zarina, Abd Wahab Norefrina Shafinaz, Md Nor Nazlina, Ibrahim QR Microbiology This study aimed to investigate the potential effect of styrylpyrone derivative (SPD) extracted from Goniothalamus umbrosus root against in vitro dengue virus type 2 (DENV-2) replication. In the present study. cytotoxicity of SPD was evaluated using a cell viability assay. Antiviral activity of SPD, was evaluated against different stages of DENV-2 replication in Vero cells using focus forming unit reduction assay Cytotoxicity test of SPD in Vero cells showed that the concentration for 50% of cytotoxicity (CCso) was 42.5 pM (8.5 pg/mL), this indicates that SPD is non-cytotoxic. SPD 50% Effective Concentration (ECso) against DENV-2 was 2.18 pM (0.436 pg/mL) which was less than the CCso value with Selectivity Index (SI) of more than 19. A high SI suggests that SPD would has good antiviral properties. The action-mode study involves penetration, adhesion, time additional, time removal and progeny infectivity test. SPD time addition test has been performed at different time intervals. SPD activity against DENV-2 was found to be most optimum when given at 2 h.p.i. which caused more than 80% focus forming reduction. In time removal assay, treatment with SPO caused 29% focus forming reduction as early as 2 h.p.i. and reached more than 80% after 96 h.p.i. This means that SPD can prevent DENV-2 activity during its earliest replication phase. SPD antivirus activity on DENV-2 attachment process is high because the focus forming reduction percentage is 80% by 25 pM SPD. Results show that SPD mechanism of action in decreasing progeny infectivity is high because the highest percentage value of inhibition percentage is greater than 100%. The results revealed that SPD exerts potent antiviral activity against DENV-2. SPD inhibits intracellular replication of DENV-2 besides possessing direct virucidal activity and reduced DENV-2 adsorption. SPO has good potential for prospective nature-based antiviral agent against dengue virus. 2019 Conference or Workshop Item NonPeerReviewed text en http://eprints.unisza.edu.my/1894/1/FH03-FSK-20-36479.pdf Noor Zarina, Abd Wahab and Norefrina Shafinaz, Md Nor and Nazlina, Ibrahim (2019) Styrylpyrone derivative of goniothalamus umbrosus: new potential in antiviral activity. In: International Conference of The Malaysian Society for Microbiology, 11-14 November 2019, Royale Chulan Seremban, Malaysia.
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
topic QR Microbiology
spellingShingle QR Microbiology
Noor Zarina, Abd Wahab
Norefrina Shafinaz, Md Nor
Nazlina, Ibrahim
Styrylpyrone derivative of goniothalamus umbrosus: new potential in antiviral activity
description This study aimed to investigate the potential effect of styrylpyrone derivative (SPD) extracted from Goniothalamus umbrosus root against in vitro dengue virus type 2 (DENV-2) replication. In the present study. cytotoxicity of SPD was evaluated using a cell viability assay. Antiviral activity of SPD, was evaluated against different stages of DENV-2 replication in Vero cells using focus forming unit reduction assay Cytotoxicity test of SPD in Vero cells showed that the concentration for 50% of cytotoxicity (CCso) was 42.5 pM (8.5 pg/mL), this indicates that SPD is non-cytotoxic. SPD 50% Effective Concentration (ECso) against DENV-2 was 2.18 pM (0.436 pg/mL) which was less than the CCso value with Selectivity Index (SI) of more than 19. A high SI suggests that SPD would has good antiviral properties. The action-mode study involves penetration, adhesion, time additional, time removal and progeny infectivity test. SPD time addition test has been performed at different time intervals. SPD activity against DENV-2 was found to be most optimum when given at 2 h.p.i. which caused more than 80% focus forming reduction. In time removal assay, treatment with SPO caused 29% focus forming reduction as early as 2 h.p.i. and reached more than 80% after 96 h.p.i. This means that SPD can prevent DENV-2 activity during its earliest replication phase. SPD antivirus activity on DENV-2 attachment process is high because the focus forming reduction percentage is 80% by 25 pM SPD. Results show that SPD mechanism of action in decreasing progeny infectivity is high because the highest percentage value of inhibition percentage is greater than 100%. The results revealed that SPD exerts potent antiviral activity against DENV-2. SPD inhibits intracellular replication of DENV-2 besides possessing direct virucidal activity and reduced DENV-2 adsorption. SPO has good potential for prospective nature-based antiviral agent against dengue virus.
format Conference or Workshop Item
author Noor Zarina, Abd Wahab
Norefrina Shafinaz, Md Nor
Nazlina, Ibrahim
author_facet Noor Zarina, Abd Wahab
Norefrina Shafinaz, Md Nor
Nazlina, Ibrahim
author_sort Noor Zarina, Abd Wahab
title Styrylpyrone derivative of goniothalamus umbrosus: new potential in antiviral activity
title_short Styrylpyrone derivative of goniothalamus umbrosus: new potential in antiviral activity
title_full Styrylpyrone derivative of goniothalamus umbrosus: new potential in antiviral activity
title_fullStr Styrylpyrone derivative of goniothalamus umbrosus: new potential in antiviral activity
title_full_unstemmed Styrylpyrone derivative of goniothalamus umbrosus: new potential in antiviral activity
title_sort styrylpyrone derivative of goniothalamus umbrosus: new potential in antiviral activity
publishDate 2019
url http://eprints.unisza.edu.my/1894/1/FH03-FSK-20-36479.pdf
http://eprints.unisza.edu.my/1894/
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score 13.211869

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