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Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production

Large numbers of glycerol-consuming bacteria are present in nature; hence bioconversion of glycerol into biofuel which is bioethanol is one of the interests. The effective screening procedure is needed to screen and isolate broad ranges of bacteria from environment. The screening method was modified...

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Main Authors: Sheril Norliana Suhaimi,, Nur Amelia Azreen Adnan,, Yee, Phang Lai, Suraini Abd-Aziz,, Maeda, Toshinari
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2016
Online Access:http://journalarticle.ukm.my/9886/1/18_Sheril_Norliana_Suhaimi.pdf
http://journalarticle.ukm.my/9886/
http://www.ukm.my/jsm/english_journals/vol45num5_2016/contentsVol45num5_2016.html
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spelling my-ukm.journal.98862016-12-14T06:51:09Z http://journalarticle.ukm.my/9886/ Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production Sheril Norliana Suhaimi, Nur Amelia Azreen Adnan, Yee, Phang Lai Suraini Abd-Aziz, Maeda, Toshinari Large numbers of glycerol-consuming bacteria are present in nature; hence bioconversion of glycerol into biofuel which is bioethanol is one of the interests. The effective screening procedure is needed to screen and isolate broad ranges of bacteria from environment. The screening method was modified based on enzymatic oxidation of ethanol, which is correlated to reduction of 2,6-dichlorophenol-indophenol dye that resulted in the formation of yellow zone. Approximately 300 colonies were able to grow on minimal media using glycerol as sole carbon. Only about 70 isolates showed positive result when using the modified ethanol production assay after pre-screening stage. The formation of decolourized zone was apparent using modified assay containing 5 mL/L of 0.05M 2,6-dichlorophenol-indophenol, 10 mL of reaction mixture and 500 μl/L of enzyme, respectively. The ethanol production capability of the isolates was further proven by anaerobic fermentation as a quantitative method. This modified method is applicable in screening for ethanol producer from glycerol as carbon source allows rapid and more bacteria can be screened. Penerbit Universiti Kebangsaan Malaysia 2016-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9886/1/18_Sheril_Norliana_Suhaimi.pdf Sheril Norliana Suhaimi, and Nur Amelia Azreen Adnan, and Yee, Phang Lai and Suraini Abd-Aziz, and Maeda, Toshinari (2016) Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production. Sains Malaysiana, 45 (5). pp. 811-815. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol45num5_2016/contentsVol45num5_2016.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Large numbers of glycerol-consuming bacteria are present in nature; hence bioconversion of glycerol into biofuel which is bioethanol is one of the interests. The effective screening procedure is needed to screen and isolate broad ranges of bacteria from environment. The screening method was modified based on enzymatic oxidation of ethanol, which is correlated to reduction of 2,6-dichlorophenol-indophenol dye that resulted in the formation of yellow zone. Approximately 300 colonies were able to grow on minimal media using glycerol as sole carbon. Only about 70 isolates showed positive result when using the modified ethanol production assay after pre-screening stage. The formation of decolourized zone was apparent using modified assay containing 5 mL/L of 0.05M 2,6-dichlorophenol-indophenol, 10 mL of reaction mixture and 500 μl/L of enzyme, respectively. The ethanol production capability of the isolates was further proven by anaerobic fermentation as a quantitative method. This modified method is applicable in screening for ethanol producer from glycerol as carbon source allows rapid and more bacteria can be screened.
format Article
author Sheril Norliana Suhaimi,
Nur Amelia Azreen Adnan,
Yee, Phang Lai
Suraini Abd-Aziz,
Maeda, Toshinari
spellingShingle Sheril Norliana Suhaimi,
Nur Amelia Azreen Adnan,
Yee, Phang Lai
Suraini Abd-Aziz,
Maeda, Toshinari
Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
author_facet Sheril Norliana Suhaimi,
Nur Amelia Azreen Adnan,
Yee, Phang Lai
Suraini Abd-Aziz,
Maeda, Toshinari
author_sort Sheril Norliana Suhaimi,
title Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
title_short Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
title_full Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
title_fullStr Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
title_full_unstemmed Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
title_sort rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2016
url http://journalarticle.ukm.my/9886/1/18_Sheril_Norliana_Suhaimi.pdf
http://journalarticle.ukm.my/9886/
http://www.ukm.my/jsm/english_journals/vol45num5_2016/contentsVol45num5_2016.html
_version_ 1643737940959952896
score 13.211869

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