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Compounds from the antioxidant active fraction of M. platytyrea

This article discusses on the natural compounds from the ant plant (Myrmecodia species, family: Rubiaceae). The ethyl acetate (EtOAc) extract from the tuber of M. platytyrea was fractionated by using medium pressure liquid chromatography, giving eight fractions (F1-F8). Those fractions were evalua...

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Main Authors: Nur Fadhilah Mohamad Haris,, Mohd Kamal Nik Hasan,, Ibtisam Abdul Wahab,, Mizaton Hazizul Hasan,, Thellie Ponto,, Aishah Adam,
Format: Article
Language:English
Published: Penerbit UKM 2016
Online Access:http://journalarticle.ukm.my/9726/1/12267-33457-1-SM.pdf
http://journalarticle.ukm.my/9726/
http://ejournal.ukm.my/jskm/issue/view/633
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spelling my-ukm.journal.97262016-12-14T06:50:39Z http://journalarticle.ukm.my/9726/ Compounds from the antioxidant active fraction of M. platytyrea Nur Fadhilah Mohamad Haris, Mohd Kamal Nik Hasan, Ibtisam Abdul Wahab, Mizaton Hazizul Hasan, Thellie Ponto, Aishah Adam, This article discusses on the natural compounds from the ant plant (Myrmecodia species, family: Rubiaceae). The ethyl acetate (EtOAc) extract from the tuber of M. platytyrea was fractionated by using medium pressure liquid chromatography, giving eight fractions (F1-F8). Those fractions were evaluated using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. Fraction F5 was recorded as potent (EC50 = 21.57 ± 1.40 µg/mL). Then, it was purified by using column chromatography (CC) (mobile phase = chloroform: EtOAc). From the CC, ten fractions (F5F1-F5F10) were obtained and compound (1) was isolated from F5F3 via preparative thin layer chromatography (TLC). After spraying with anisaldehyde-sulphuric reagent, compound (1) gave a green TLC spot (Rf = 0.65, 100% CHCl3 , multiple development). The 1 H-Nuclear Magnetic Resonance (NMR) spectroscopy (500 MHz, CDCl3 ) was performed to determine the chemical framework of (1). This compound was identified as morindolide, having an iridoid structure. Meanwhile, the mass spectra for compounds (2) and (3) were analysed. The data presented the molecular ion at m/z 375 [M-H]- and 255, suggesting the formulation of 2-(2-methylbutyryl)phloroglucinol glucoside and a flavanone, respectively. From the literature, compound (1) was firstly isolated from a Chinese natural medicine, the dried root of Morinda officinalis (family: Rubiaceae). The flavonoids are also included as the biologically active compounds from Myrmecodia. In short, this is the first occurrence of morindolide from the ant plant. Penerbit UKM 2016 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9726/1/12267-33457-1-SM.pdf Nur Fadhilah Mohamad Haris, and Mohd Kamal Nik Hasan, and Ibtisam Abdul Wahab, and Mizaton Hazizul Hasan, and Thellie Ponto, and Aishah Adam, (2016) Compounds from the antioxidant active fraction of M. platytyrea. Jurnal Sains Kesihatan Malaysia, 14 (1). pp. 23-29. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/633
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description This article discusses on the natural compounds from the ant plant (Myrmecodia species, family: Rubiaceae). The ethyl acetate (EtOAc) extract from the tuber of M. platytyrea was fractionated by using medium pressure liquid chromatography, giving eight fractions (F1-F8). Those fractions were evaluated using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. Fraction F5 was recorded as potent (EC50 = 21.57 ± 1.40 µg/mL). Then, it was purified by using column chromatography (CC) (mobile phase = chloroform: EtOAc). From the CC, ten fractions (F5F1-F5F10) were obtained and compound (1) was isolated from F5F3 via preparative thin layer chromatography (TLC). After spraying with anisaldehyde-sulphuric reagent, compound (1) gave a green TLC spot (Rf = 0.65, 100% CHCl3 , multiple development). The 1 H-Nuclear Magnetic Resonance (NMR) spectroscopy (500 MHz, CDCl3 ) was performed to determine the chemical framework of (1). This compound was identified as morindolide, having an iridoid structure. Meanwhile, the mass spectra for compounds (2) and (3) were analysed. The data presented the molecular ion at m/z 375 [M-H]- and 255, suggesting the formulation of 2-(2-methylbutyryl)phloroglucinol glucoside and a flavanone, respectively. From the literature, compound (1) was firstly isolated from a Chinese natural medicine, the dried root of Morinda officinalis (family: Rubiaceae). The flavonoids are also included as the biologically active compounds from Myrmecodia. In short, this is the first occurrence of morindolide from the ant plant.
format Article
author Nur Fadhilah Mohamad Haris,
Mohd Kamal Nik Hasan,
Ibtisam Abdul Wahab,
Mizaton Hazizul Hasan,
Thellie Ponto,
Aishah Adam,
spellingShingle Nur Fadhilah Mohamad Haris,
Mohd Kamal Nik Hasan,
Ibtisam Abdul Wahab,
Mizaton Hazizul Hasan,
Thellie Ponto,
Aishah Adam,
Compounds from the antioxidant active fraction of M. platytyrea
author_facet Nur Fadhilah Mohamad Haris,
Mohd Kamal Nik Hasan,
Ibtisam Abdul Wahab,
Mizaton Hazizul Hasan,
Thellie Ponto,
Aishah Adam,
author_sort Nur Fadhilah Mohamad Haris,
title Compounds from the antioxidant active fraction of M. platytyrea
title_short Compounds from the antioxidant active fraction of M. platytyrea
title_full Compounds from the antioxidant active fraction of M. platytyrea
title_fullStr Compounds from the antioxidant active fraction of M. platytyrea
title_full_unstemmed Compounds from the antioxidant active fraction of M. platytyrea
title_sort compounds from the antioxidant active fraction of m. platytyrea
publisher Penerbit UKM
publishDate 2016
url http://journalarticle.ukm.my/9726/1/12267-33457-1-SM.pdf
http://journalarticle.ukm.my/9726/
http://ejournal.ukm.my/jskm/issue/view/633
_version_ 1643737882498695168
score 13.211869

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