Matrine enhances the inhibitory effect of 5-FU on SW480 cells in vitro and in vivo
We investigated the antitumor effects of the combination of matrine-a purified alkaloid extracted from Sophora flavescence-and 5-fluorouracil (5-FU) on SW480 cells. This combination inhibited the growth of SW480 cells in a synergistic or additive manner by disrupting their progression through the ce...
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Universiti Kebangsaan Malaysia
2016
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my-ukm.journal.95752016-12-14T06:50:21Z http://journalarticle.ukm.my/9575/ Matrine enhances the inhibitory effect of 5-FU on SW480 cells in vitro and in vivo Xu, Hui Zhang Lei, Liang Xiao, Yan Wang Li, Zhang Yan, Xin Zheng Hong, Zhu Deng Xin, Rong Wu We investigated the antitumor effects of the combination of matrine-a purified alkaloid extracted from Sophora flavescence-and 5-fluorouracil (5-FU) on SW480 cells. This combination inhibited the growth of SW480 cells in a synergistic or additive manner by disrupting their progression through the cell cycle. Exposure of SW480 cells to matrine and 5-FU was followed by an increased rate of expression for caspase-3, caspase-9 and poly-ADP ribose polymerase (PARP) and inhibited the subcutaneous transplantation of SW480 tumors into Balb/c nude mice. Histopathological analysis showed that this effect was most pronounced in the spleens of treated animals. Typical cytotoxic effects observed in 5-FU-treated mice included fibrosis and lymphopenia, whereas in mice treated with 5-FU combined with matrine, the spleen ultrastructure remained intact. These findings indicate that matrine may enhance the therapeutic effectiveness of 5-FU in SW480 tumors by enhancing apoptosis and overcome the threat to immunocompetence associated with 5-FU. Universiti Kebangsaan Malaysia 2016-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9575/1/13_Lei_Liang.pdf Xu, Hui Zhang and Lei, Liang and Xiao, Yan Wang and Li, Zhang and Yan, Xin Zheng and Hong, Zhu Deng and Xin, Rong Wu (2016) Matrine enhances the inhibitory effect of 5-FU on SW480 cells in vitro and in vivo. Sains Malaysiana, 45 (1). pp. 109-113. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid45bil1_2016/KandunganJilid45Bil1_2016.htm |
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We investigated the antitumor effects of the combination of matrine-a purified alkaloid extracted from Sophora flavescence-and 5-fluorouracil (5-FU) on SW480 cells. This combination inhibited the growth of SW480 cells in a synergistic or additive manner by disrupting their progression through the cell cycle. Exposure of SW480 cells to matrine and 5-FU was followed by an increased rate of expression for caspase-3, caspase-9 and poly-ADP ribose polymerase (PARP) and inhibited the subcutaneous transplantation of SW480 tumors into Balb/c nude mice. Histopathological analysis showed that this effect was most pronounced in the spleens of treated animals. Typical cytotoxic effects observed in 5-FU-treated mice included fibrosis and lymphopenia, whereas in mice treated with 5-FU combined with matrine, the spleen ultrastructure remained intact. These findings indicate that matrine may enhance the therapeutic effectiveness of 5-FU in SW480 tumors by enhancing apoptosis and overcome the threat to immunocompetence associated with 5-FU. |
| format |
Article |
| author |
Xu, Hui Zhang Lei, Liang Xiao, Yan Wang Li, Zhang Yan, Xin Zheng Hong, Zhu Deng Xin, Rong Wu |
| spellingShingle |
Xu, Hui Zhang Lei, Liang Xiao, Yan Wang Li, Zhang Yan, Xin Zheng Hong, Zhu Deng Xin, Rong Wu Matrine enhances the inhibitory effect of 5-FU on SW480 cells in vitro and in vivo |
| author_facet |
Xu, Hui Zhang Lei, Liang Xiao, Yan Wang Li, Zhang Yan, Xin Zheng Hong, Zhu Deng Xin, Rong Wu |
| author_sort |
Xu, Hui Zhang |
| title |
Matrine enhances the inhibitory effect of 5-FU
on SW480 cells in vitro and in vivo |
| title_short |
Matrine enhances the inhibitory effect of 5-FU
on SW480 cells in vitro and in vivo |
| title_full |
Matrine enhances the inhibitory effect of 5-FU
on SW480 cells in vitro and in vivo |
| title_fullStr |
Matrine enhances the inhibitory effect of 5-FU
on SW480 cells in vitro and in vivo |
| title_full_unstemmed |
Matrine enhances the inhibitory effect of 5-FU
on SW480 cells in vitro and in vivo |
| title_sort |
matrine enhances the inhibitory effect of 5-fu
on sw480 cells in vitro and in vivo |
| publisher |
Universiti Kebangsaan Malaysia |
| publishDate |
2016 |
| url |
http://journalarticle.ukm.my/9575/1/13_Lei_Liang.pdf http://journalarticle.ukm.my/9575/ http://www.ukm.my/jsm/malay_journals/jilid45bil1_2016/KandunganJilid45Bil1_2016.htm |
| _version_ |
1643737846731767808 |
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13.211869 |