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Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia

Microbial communities of two oil reservoirs from Malaysia, denoted as Platform Bo and Platform Pe were studied using culture-independent approach. Environmental DNA was extracted and the universal amplified ribosomal region (UARR) was target amplified for both prokaryotes and eukaryotes. The amplifi...

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Main Authors: Cheng, Yee Fish-Low, Ainon Hamzah,, Hui, Yee Chee
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2015
Online Access:http://journalarticle.ukm.my/9372/1/13_Cheng-Yee_Fish.pdf
http://journalarticle.ukm.my/9372/
http://www.ukm.my/jsm/malay_journals/jilid44bil11_2015/KandunganJilid44Bil11_2015.html
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spelling my-ukm.journal.93722016-12-14T06:49:44Z http://journalarticle.ukm.my/9372/ Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia Cheng, Yee Fish-Low Ainon Hamzah, Hui, Yee Chee Microbial communities of two oil reservoirs from Malaysia, denoted as Platform Bo and Platform Pe were studied using culture-independent approach. Environmental DNA was extracted and the universal amplified ribosomal region (UARR) was target amplified for both prokaryotes and eukaryotes. The amplified products were purified and cloned into pTZ57R/T vector to construct the 16S/18S rDNA library. Restriction endocucleases HhaI and MspI were used to screen the library. From that, 125 and 253 recombinant plasmid representative clones from Platform Bo and Platform Pe, respectively, were sent for DNA sequencing. Twenty-six operational taxonomic units (OTUs) consist of 20 genera detected at Platform Bo and 17 OTUs consist of 13 genera detected at Platform Pe. Marinobacter and Acinetobacter species co-occurred in both platforms whereas the rest are site-specific. Gammaproteobacteria accounted for 86.0% of the microbial community in Platform Bo, where OTUs affiliated to Marinobacter, Pseudomonas and Marinobacterium that were the most abundant. The major OTUs in the Platform Pe were with affinities to Achromobacter, followed by Stenotrophomonas and Serratia. The only archaeal isolates were detected in Platform Pe, which affiliated to Thermocladium. The singletons and doubletons accounted for about 50.0% of the OTU abundance in both platforms, which considerably significant despite their rare occurrence. Universiti Kebangsaan Malaysia 2015-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9372/1/13_Cheng-Yee_Fish.pdf Cheng, Yee Fish-Low and Ainon Hamzah, and Hui, Yee Chee (2015) Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia. Sains Malaysiana, 44 (11). pp. 1625-1633. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid44bil11_2015/KandunganJilid44Bil11_2015.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Microbial communities of two oil reservoirs from Malaysia, denoted as Platform Bo and Platform Pe were studied using culture-independent approach. Environmental DNA was extracted and the universal amplified ribosomal region (UARR) was target amplified for both prokaryotes and eukaryotes. The amplified products were purified and cloned into pTZ57R/T vector to construct the 16S/18S rDNA library. Restriction endocucleases HhaI and MspI were used to screen the library. From that, 125 and 253 recombinant plasmid representative clones from Platform Bo and Platform Pe, respectively, were sent for DNA sequencing. Twenty-six operational taxonomic units (OTUs) consist of 20 genera detected at Platform Bo and 17 OTUs consist of 13 genera detected at Platform Pe. Marinobacter and Acinetobacter species co-occurred in both platforms whereas the rest are site-specific. Gammaproteobacteria accounted for 86.0% of the microbial community in Platform Bo, where OTUs affiliated to Marinobacter, Pseudomonas and Marinobacterium that were the most abundant. The major OTUs in the Platform Pe were with affinities to Achromobacter, followed by Stenotrophomonas and Serratia. The only archaeal isolates were detected in Platform Pe, which affiliated to Thermocladium. The singletons and doubletons accounted for about 50.0% of the OTU abundance in both platforms, which considerably significant despite their rare occurrence.
format Article
author Cheng, Yee Fish-Low
Ainon Hamzah,
Hui, Yee Chee
spellingShingle Cheng, Yee Fish-Low
Ainon Hamzah,
Hui, Yee Chee
Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
author_facet Cheng, Yee Fish-Low
Ainon Hamzah,
Hui, Yee Chee
author_sort Cheng, Yee Fish-Low
title Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
title_short Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
title_full Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
title_fullStr Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
title_full_unstemmed Culture-independent-based analyses identified site-specific microbial in two oil-wells from Malaysia
title_sort culture-independent-based analyses identified site-specific microbial in two oil-wells from malaysia
publisher Universiti Kebangsaan Malaysia
publishDate 2015
url http://journalarticle.ukm.my/9372/1/13_Cheng-Yee_Fish.pdf
http://journalarticle.ukm.my/9372/
http://www.ukm.my/jsm/malay_journals/jilid44bil11_2015/KandunganJilid44Bil11_2015.html
_version_ 1643737770388094976
score 13.211869

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