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Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa

The objectives of this study were to determine the effect of different cryoprotectants and sperm densities for long-term storage of orange mud crab, Scylla olivacea spermatozoa. Spermatozoa were obtained by homogenizing the spermatophores using a glass homogenizer in an ice-bath followed by centrifu...

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Main Authors: Mhd Ikhwanuddin,, Teng Phei Yin,, Abdul Jabbar Menon,, Safiah Jasmani,, Ambok Bolong Abol-Munafi,
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2015
Online Access:http://journalarticle.ukm.my/9263/1/10_Mhd_Ikhwanuddin.pdf
http://journalarticle.ukm.my/9263/
http://www.ukm.my/jsm/malay_journals/jilid44bil9_2015/KandunganJilid44Bil9_2015.html
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spelling my-ukm.journal.92632016-12-14T06:49:23Z http://journalarticle.ukm.my/9263/ Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa Mhd Ikhwanuddin, Teng Phei Yin, Abdul Jabbar Menon, Safiah Jasmani, Ambok Bolong Abol-Munafi, The objectives of this study were to determine the effect of different cryoprotectants and sperm densities for long-term storage of orange mud crab, Scylla olivacea spermatozoa. Spermatozoa were obtained by homogenizing the spermatophores using a glass homogenizer in an ice-bath followed by centrifugation at 4°C. Spermatozoa were then suspended in calcium-free saline (Ca-F saline) containing 5% of the following cryoprotectants: Glycerol, dimethyl sulfoxide (DMSO) and methanol. Sperm which vibrated and rotated were counted as live during sperm viability assessment. Samples of spermatozoa were cooled to -196°C by two-step freezing, first to -80°C and then by plunging into liquid nitrogen (LN). Spermatozoa were gradually cooled at 1°C/min. Thawing was carried out in a 30°C water bath for 2 min. This yielded live sperm after storage in LN for 30 days. The best sperm viability was obtained from a density of 108 cells per mL in DMSO. There was no significant difference (p>0.05) among cryoprotectants toward sperm viability. However, sperm viability was significantly affected (p>0.05) by cell densities. In conclusion, DMSO gave the best protection to sperm cells of S. olivacea, but the effectiveness of DMSO as a cryoprotectant is influenced by sperm density. Universiti Kebangsaan Malaysia 2015-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9263/1/10_Mhd_Ikhwanuddin.pdf Mhd Ikhwanuddin, and Teng Phei Yin, and Abdul Jabbar Menon, and Safiah Jasmani, and Ambok Bolong Abol-Munafi, (2015) Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa. Sains Malaysiana, 44 (9). pp. 1283-1288. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid44bil9_2015/KandunganJilid44Bil9_2015.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description The objectives of this study were to determine the effect of different cryoprotectants and sperm densities for long-term storage of orange mud crab, Scylla olivacea spermatozoa. Spermatozoa were obtained by homogenizing the spermatophores using a glass homogenizer in an ice-bath followed by centrifugation at 4°C. Spermatozoa were then suspended in calcium-free saline (Ca-F saline) containing 5% of the following cryoprotectants: Glycerol, dimethyl sulfoxide (DMSO) and methanol. Sperm which vibrated and rotated were counted as live during sperm viability assessment. Samples of spermatozoa were cooled to -196°C by two-step freezing, first to -80°C and then by plunging into liquid nitrogen (LN). Spermatozoa were gradually cooled at 1°C/min. Thawing was carried out in a 30°C water bath for 2 min. This yielded live sperm after storage in LN for 30 days. The best sperm viability was obtained from a density of 108 cells per mL in DMSO. There was no significant difference (p>0.05) among cryoprotectants toward sperm viability. However, sperm viability was significantly affected (p>0.05) by cell densities. In conclusion, DMSO gave the best protection to sperm cells of S. olivacea, but the effectiveness of DMSO as a cryoprotectant is influenced by sperm density.
format Article
author Mhd Ikhwanuddin,
Teng Phei Yin,
Abdul Jabbar Menon,
Safiah Jasmani,
Ambok Bolong Abol-Munafi,
spellingShingle Mhd Ikhwanuddin,
Teng Phei Yin,
Abdul Jabbar Menon,
Safiah Jasmani,
Ambok Bolong Abol-Munafi,
Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa
author_facet Mhd Ikhwanuddin,
Teng Phei Yin,
Abdul Jabbar Menon,
Safiah Jasmani,
Ambok Bolong Abol-Munafi,
author_sort Mhd Ikhwanuddin,
title Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa
title_short Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa
title_full Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa
title_fullStr Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa
title_full_unstemmed Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa
title_sort effect of different cryoprotectants and sperm densities of orange mud crab, scylla olivacea (herbst, 1796) for long-term storage of spermatozoa
publisher Universiti Kebangsaan Malaysia
publishDate 2015
url http://journalarticle.ukm.my/9263/1/10_Mhd_Ikhwanuddin.pdf
http://journalarticle.ukm.my/9263/
http://www.ukm.my/jsm/malay_journals/jilid44bil9_2015/KandunganJilid44Bil9_2015.html
_version_ 1643737734173425664
score 13.211869

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