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Effects of salvadora persica extract on the bacterial population in single-species biofilm

The effect of S. persica extract on the population of bacteria in single-species biofilm was investigated. An artificial mouth (NAM) system was used for the development of biofilm. In one experiment, aqueous extract of S. persica at sub-MIC concentration (5 mg/mL) was first pumped into the NAM syste...

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Main Authors: Nordin Fatin-Majdina,, Haji Abd Rahim Zubaidah,, Abd Razak Monica@Munirah,, Mohd Bakri Marina,
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2014
Online Access:http://journalarticle.ukm.my/8149/1/10_Nordin_Fatin.pdf
http://journalarticle.ukm.my/8149/
http://www.ukm.my/jsm/
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spelling my-ukm.journal.81492016-12-14T06:46:22Z http://journalarticle.ukm.my/8149/ Effects of salvadora persica extract on the bacterial population in single-species biofilm Nordin Fatin-Majdina, Haji Abd Rahim Zubaidah, Abd Razak Monica@Munirah, Mohd Bakri Marina, The effect of S. persica extract on the population of bacteria in single-species biofilm was investigated. An artificial mouth (NAM) system was used for the development of biofilm. In one experiment, aqueous extract of S. persica at sub-MIC concentration (5 mg/mL) was first pumped into the NAM system to treat the experimental pellicle on glass beads before inoculating it with the bacteria (Strep. mitis, Strep. mutans and Strep. sanguinis). This would resemble using the aqueous extract of S. persica before meals. In another experiment, the aqueous extract of S. persica was pumped into the NAM system after the bacterial inoculation and this would resemble using it after meals. In both experiments, 24 h biofilms were allowed to form. The bacterial population of the biofilms was determined and expressed as colony forming unit per mL (cfu mL-1). For negative control, sterilized distilled water was used whereas for positive control, commercial Listerine. It was found that the population of Strep. mitis, Strep. mutans and Strep. sanguinis in the respective biofilms for both experiments involving treatment with S. persica extract or Listerine was significantly reduced by more than 70% (p<0.05) when compared with the negative control. Comparing the effect of S. persica with Listerine on the bacterial population of the biofilms when used either before or after meal showed that S. persica is slightly less effective towards S. sanguinis before meal and towards S. mutans after meal (p<0.05). This study suggests that the efficiency of the antibacterial effect of S. persica is species-related and by its way of usage. Universiti Kebangsaan Malaysia 2014-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8149/1/10_Nordin_Fatin.pdf Nordin Fatin-Majdina, and Haji Abd Rahim Zubaidah, and Abd Razak Monica@Munirah, and Mohd Bakri Marina, (2014) Effects of salvadora persica extract on the bacterial population in single-species biofilm. Sains Malaysiana, 43 (12). pp. 1889-1893. ISSN 0126-6039 http://www.ukm.my/jsm/
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description The effect of S. persica extract on the population of bacteria in single-species biofilm was investigated. An artificial mouth (NAM) system was used for the development of biofilm. In one experiment, aqueous extract of S. persica at sub-MIC concentration (5 mg/mL) was first pumped into the NAM system to treat the experimental pellicle on glass beads before inoculating it with the bacteria (Strep. mitis, Strep. mutans and Strep. sanguinis). This would resemble using the aqueous extract of S. persica before meals. In another experiment, the aqueous extract of S. persica was pumped into the NAM system after the bacterial inoculation and this would resemble using it after meals. In both experiments, 24 h biofilms were allowed to form. The bacterial population of the biofilms was determined and expressed as colony forming unit per mL (cfu mL-1). For negative control, sterilized distilled water was used whereas for positive control, commercial Listerine. It was found that the population of Strep. mitis, Strep. mutans and Strep. sanguinis in the respective biofilms for both experiments involving treatment with S. persica extract or Listerine was significantly reduced by more than 70% (p<0.05) when compared with the negative control. Comparing the effect of S. persica with Listerine on the bacterial population of the biofilms when used either before or after meal showed that S. persica is slightly less effective towards S. sanguinis before meal and towards S. mutans after meal (p<0.05). This study suggests that the efficiency of the antibacterial effect of S. persica is species-related and by its way of usage.
format Article
author Nordin Fatin-Majdina,
Haji Abd Rahim Zubaidah,
Abd Razak Monica@Munirah,
Mohd Bakri Marina,
spellingShingle Nordin Fatin-Majdina,
Haji Abd Rahim Zubaidah,
Abd Razak Monica@Munirah,
Mohd Bakri Marina,
Effects of salvadora persica extract on the bacterial population in single-species biofilm
author_facet Nordin Fatin-Majdina,
Haji Abd Rahim Zubaidah,
Abd Razak Monica@Munirah,
Mohd Bakri Marina,
author_sort Nordin Fatin-Majdina,
title Effects of salvadora persica extract on the bacterial population in single-species biofilm
title_short Effects of salvadora persica extract on the bacterial population in single-species biofilm
title_full Effects of salvadora persica extract on the bacterial population in single-species biofilm
title_fullStr Effects of salvadora persica extract on the bacterial population in single-species biofilm
title_full_unstemmed Effects of salvadora persica extract on the bacterial population in single-species biofilm
title_sort effects of salvadora persica extract on the bacterial population in single-species biofilm
publisher Universiti Kebangsaan Malaysia
publishDate 2014
url http://journalarticle.ukm.my/8149/1/10_Nordin_Fatin.pdf
http://journalarticle.ukm.my/8149/
http://www.ukm.my/jsm/
_version_ 1643737376019709952
score 13.211869

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