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Antioxidant, anticancer and antimicrobial activities of methanolic extracts from enicosanthellum pulchrum (King) heusden

Biological activities of crude methanolic extracts from leaves, barks, twigs and roots of Enicosanthellum pulchrum were investigated in four bioassays. The antioxidant, 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging assay showed that bark and twig extracts showed high inhibitory activity with 60 an...

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Main Authors: Noraziah Nordin,, Syam Mohan,, Najihah Mohd. Hashim,, Asdren Zajm,, Noor Shafifiyaz Mohd. Yazid,, Mashitoh Abdul Rahman,, Fatima Abdelmutaal Ahmed Omer,, Hanita Omar,, Fatimah A'fifah Alias,, Hapipah Mohd. Ali,
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2014
Online Access:http://journalarticle.ukm.my/7814/1/08_Noraziah_Nordin.pdf
http://journalarticle.ukm.my/7814/
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spelling my-ukm.journal.78142016-12-14T06:45:17Z http://journalarticle.ukm.my/7814/ Antioxidant, anticancer and antimicrobial activities of methanolic extracts from enicosanthellum pulchrum (King) heusden Noraziah Nordin, Syam Mohan, Najihah Mohd. Hashim, Asdren Zajm, Noor Shafifiyaz Mohd. Yazid, Mashitoh Abdul Rahman, Fatima Abdelmutaal Ahmed Omer, Hanita Omar, Fatimah A'fifah Alias, Hapipah Mohd. Ali, Biological activities of crude methanolic extracts from leaves, barks, twigs and roots of Enicosanthellum pulchrum were investigated in four bioassays. The antioxidant, 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging assay showed that bark and twig extracts showed high inhibitory activity with 60 and 56% inhibition at 1 mg/mL and IC50 values of 0.43 ± 0.04 and 0.64 ± 0.05 mg/mL, respectively. The bark and root extracts showed greater reducing power (FRAP) than several standard drugs used in the bioassay. Methanolic extracts of leaves, twigs and roots displayed strong cytotoxicity to breast cancer cell line (MCF-7), myelomonocytic leukaemia cell line (WEHI-3) and ovarian cancer cell line (CAOV-3); the IC50 of the leaf extract were 7.8 ± 0.85 μg/mL (MCF-7) and 9.0 ± 0.13 μg/mL (WEHI-3), while those for the twig and root extracts were 13.9 ± 0.35 and 7.3 ± 0.98 μg/mL (CAOV-3), respectively. In the antimicrobial assays, the extracts were tested against ten bacterial strains and two fungal strains. Bark and twig extracts displayed high inhibitory activity to Bacillus subtilis with 13.3 ± 0.57 and 12.0 ± 0.01 mm inhibition, respectively. In addition, the twig extract displayed better minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) compared with the bark extract (MIC 0.5 and 1.0 mg/mL, MBC 1.0 and 2.0 mg/mL, respectively). For antifungal activity, all extracts showed inhibition on Candida albicans but not on Aspergillus niger. The obtained results suggested that this plant may possibly contain bioactive compounds in the active extracts. Universiti Kebangsaan Malaysia 2014-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7814/1/08_Noraziah_Nordin.pdf Noraziah Nordin, and Syam Mohan, and Najihah Mohd. Hashim, and Asdren Zajm, and Noor Shafifiyaz Mohd. Yazid, and Mashitoh Abdul Rahman, and Fatima Abdelmutaal Ahmed Omer, and Hanita Omar, and Fatimah A'fifah Alias, and Hapipah Mohd. Ali, (2014) Antioxidant, anticancer and antimicrobial activities of methanolic extracts from enicosanthellum pulchrum (King) heusden. Sains Malaysiana, 43 (10). pp. 1515-1521. ISSN 0126-6039 http://www.ukm.my/jsm/
institution Universiti Kebangsaan Malaysia
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country Malaysia
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description Biological activities of crude methanolic extracts from leaves, barks, twigs and roots of Enicosanthellum pulchrum were investigated in four bioassays. The antioxidant, 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging assay showed that bark and twig extracts showed high inhibitory activity with 60 and 56% inhibition at 1 mg/mL and IC50 values of 0.43 ± 0.04 and 0.64 ± 0.05 mg/mL, respectively. The bark and root extracts showed greater reducing power (FRAP) than several standard drugs used in the bioassay. Methanolic extracts of leaves, twigs and roots displayed strong cytotoxicity to breast cancer cell line (MCF-7), myelomonocytic leukaemia cell line (WEHI-3) and ovarian cancer cell line (CAOV-3); the IC50 of the leaf extract were 7.8 ± 0.85 μg/mL (MCF-7) and 9.0 ± 0.13 μg/mL (WEHI-3), while those for the twig and root extracts were 13.9 ± 0.35 and 7.3 ± 0.98 μg/mL (CAOV-3), respectively. In the antimicrobial assays, the extracts were tested against ten bacterial strains and two fungal strains. Bark and twig extracts displayed high inhibitory activity to Bacillus subtilis with 13.3 ± 0.57 and 12.0 ± 0.01 mm inhibition, respectively. In addition, the twig extract displayed better minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) compared with the bark extract (MIC 0.5 and 1.0 mg/mL, MBC 1.0 and 2.0 mg/mL, respectively). For antifungal activity, all extracts showed inhibition on Candida albicans but not on Aspergillus niger. The obtained results suggested that this plant may possibly contain bioactive compounds in the active extracts.
format Article
author Noraziah Nordin,
Syam Mohan,
Najihah Mohd. Hashim,
Asdren Zajm,
Noor Shafifiyaz Mohd. Yazid,
Mashitoh Abdul Rahman,
Fatima Abdelmutaal Ahmed Omer,
Hanita Omar,
Fatimah A'fifah Alias,
Hapipah Mohd. Ali,
spellingShingle Noraziah Nordin,
Syam Mohan,
Najihah Mohd. Hashim,
Asdren Zajm,
Noor Shafifiyaz Mohd. Yazid,
Mashitoh Abdul Rahman,
Fatima Abdelmutaal Ahmed Omer,
Hanita Omar,
Fatimah A'fifah Alias,
Hapipah Mohd. Ali,
Antioxidant, anticancer and antimicrobial activities of methanolic extracts from enicosanthellum pulchrum (King) heusden
author_facet Noraziah Nordin,
Syam Mohan,
Najihah Mohd. Hashim,
Asdren Zajm,
Noor Shafifiyaz Mohd. Yazid,
Mashitoh Abdul Rahman,
Fatima Abdelmutaal Ahmed Omer,
Hanita Omar,
Fatimah A'fifah Alias,
Hapipah Mohd. Ali,
author_sort Noraziah Nordin,
title Antioxidant, anticancer and antimicrobial activities of methanolic extracts from enicosanthellum pulchrum (King) heusden
title_short Antioxidant, anticancer and antimicrobial activities of methanolic extracts from enicosanthellum pulchrum (King) heusden
title_full Antioxidant, anticancer and antimicrobial activities of methanolic extracts from enicosanthellum pulchrum (King) heusden
title_fullStr Antioxidant, anticancer and antimicrobial activities of methanolic extracts from enicosanthellum pulchrum (King) heusden
title_full_unstemmed Antioxidant, anticancer and antimicrobial activities of methanolic extracts from enicosanthellum pulchrum (King) heusden
title_sort antioxidant, anticancer and antimicrobial activities of methanolic extracts from enicosanthellum pulchrum (king) heusden
publisher Universiti Kebangsaan Malaysia
publishDate 2014
url http://journalarticle.ukm.my/7814/1/08_Noraziah_Nordin.pdf
http://journalarticle.ukm.my/7814/
http://www.ukm.my/jsm/
_version_ 1643737255329660928
score 13.211869

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