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Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway

Recent studies suggest that reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) may exert beneficial effects on health. Therefore this study evaluated the effects of H2O2 and gamma-tocotrienol (GTT), an antioxidant, on D-[2-3H]Glucose uptake by myotubes compared to insulin. Results showed...

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Main Authors: Musalmah Mazlan,, Rehanna Mansor,, Wan Zurinah Wan Ngah,
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2010
Online Access:http://journalarticle.ukm.my/7338/1/01_Md_Yeaminhossain.pdf
http://journalarticle.ukm.my/7338/
http://www.ukm.my/jsm/english_journals/vol39num2_2010/contentsVol39num2_2010.html
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spelling my-ukm.journal.73382016-12-14T06:43:47Z http://journalarticle.ukm.my/7338/ Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway Musalmah Mazlan, Rehanna Mansor, Wan Zurinah Wan Ngah, Recent studies suggest that reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) may exert beneficial effects on health. Therefore this study evaluated the effects of H2O2 and gamma-tocotrienol (GTT), an antioxidant, on D-[2-3H]Glucose uptake by myotubes compared to insulin. Results showed that H2O2 and insulin significantly increased D-[2-3H]Glucose uptake. This was associated with an increased in Vmax but Michaelis constant (Km) of the transport system remained unchanged indicating that an increase in amount, rather than affinity, of the glucose transporter was involved in the process. Western blot studies confirmed that H2O2 increased the expressions of insulin sensitive glucose transporter, GLUT4 and also hexokinase (an enzyme which phosphorylates glucose and indirectly stimulates glucose uptake by cells). H2O2 and insulin also stimulated increased in PPAR-γ and IRS-1, which are proteins of the insulin transduction pathway. The present data however showed that GTT did not exert any effect on glucose uptake. Thus, the study showed that H2O2 mimicked insulin action in stimulating glucose uptake in myotube cells by influencing the insulin signaling pathway. Universiti Kebangsaan Malaysia 2010 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7338/1/01_Md_Yeaminhossain.pdf Musalmah Mazlan, and Rehanna Mansor, and Wan Zurinah Wan Ngah, (2010) Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway. Sains Malaysiana, 39 (2). pp. 315-320. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol39num2_2010/contentsVol39num2_2010.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Recent studies suggest that reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) may exert beneficial effects on health. Therefore this study evaluated the effects of H2O2 and gamma-tocotrienol (GTT), an antioxidant, on D-[2-3H]Glucose uptake by myotubes compared to insulin. Results showed that H2O2 and insulin significantly increased D-[2-3H]Glucose uptake. This was associated with an increased in Vmax but Michaelis constant (Km) of the transport system remained unchanged indicating that an increase in amount, rather than affinity, of the glucose transporter was involved in the process. Western blot studies confirmed that H2O2 increased the expressions of insulin sensitive glucose transporter, GLUT4 and also hexokinase (an enzyme which phosphorylates glucose and indirectly stimulates glucose uptake by cells). H2O2 and insulin also stimulated increased in PPAR-γ and IRS-1, which are proteins of the insulin transduction pathway. The present data however showed that GTT did not exert any effect on glucose uptake. Thus, the study showed that H2O2 mimicked insulin action in stimulating glucose uptake in myotube cells by influencing the insulin signaling pathway.
format Article
author Musalmah Mazlan,
Rehanna Mansor,
Wan Zurinah Wan Ngah,
spellingShingle Musalmah Mazlan,
Rehanna Mansor,
Wan Zurinah Wan Ngah,
Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway
author_facet Musalmah Mazlan,
Rehanna Mansor,
Wan Zurinah Wan Ngah,
author_sort Musalmah Mazlan,
title Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway
title_short Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway
title_full Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway
title_fullStr Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway
title_full_unstemmed Hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway
title_sort hydrogen peroxide stimulates glucose uptake in myotube cells via the insulin signaling pathway
publisher Universiti Kebangsaan Malaysia
publishDate 2010
url http://journalarticle.ukm.my/7338/1/01_Md_Yeaminhossain.pdf
http://journalarticle.ukm.my/7338/
http://www.ukm.my/jsm/english_journals/vol39num2_2010/contentsVol39num2_2010.html
_version_ 1643737089009778688
score 13.211869

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