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Isolation and characterization of bacteria degrading Sumandak and South Angsi Oils

Four species of bacteria, Acinetobacter lwoffii, Aeromonas hydrophila, Pseudomonas aeruginosa and Pseudomonas putida, were isolated from soil contaminated with hydrocarbons and selected for the determination of growth requirements and the ability to degrade petroleum hydrocarbon. The bacteria were g...

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Main Authors: Ainon Hamzah,, Amir Rabu,, Raja Farzarul Hanim Raja Azmy,, Noor Aini Yussoff,
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2010
Online Access:http://journalarticle.ukm.my/7315/1/01_Md_Yeaminhossain.pdf
http://journalarticle.ukm.my/7315/
http://www.ukm.my/jsm/english_journals/vol39num2_2010/contentsVol39num2_2010.html
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spelling my-ukm.journal.73152016-12-14T06:43:43Z http://journalarticle.ukm.my/7315/ Isolation and characterization of bacteria degrading Sumandak and South Angsi Oils Ainon Hamzah, Amir Rabu, Raja Farzarul Hanim Raja Azmy, Noor Aini Yussoff, Four species of bacteria, Acinetobacter lwoffii, Aeromonas hydrophila, Pseudomonas aeruginosa and Pseudomonas putida, were isolated from soil contaminated with hydrocarbons and selected for the determination of growth requirements and the ability to degrade petroleum hydrocarbon. The bacteria were grown in mineral salt medium (MSM) supplemented with two types of crude oil, either Sumandak or South Angsi at 1% (v/v) concentration. The optimum pH for growth of A. hydrophila and P. aeruginosa was 6.5 when grown with Sumandak and South Angsi oil. For A. lwoffii and P. putida the optimum pH for growth with Sumandak and South Angsi oil was 6.5 or 7.0, respectively. The growth of P. aeruginosa was the highest in MSM when supplemented with 1% South Angsi oil and 0.5% tryptone at pH 6.5 while, in Sumandak oil the growth was the highest when yeast extract was added. Gas chromatography analysis showed that the South Angsi crude oil components of C12 to C25 were more extensively degraded by A. lwoffii after 24 h of incubation compared to the other bacteria over the same period. Universiti Kebangsaan Malaysia 2010-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7315/1/01_Md_Yeaminhossain.pdf Ainon Hamzah, and Amir Rabu, and Raja Farzarul Hanim Raja Azmy, and Noor Aini Yussoff, (2010) Isolation and characterization of bacteria degrading Sumandak and South Angsi Oils. Sains Malaysiana, 39 (2). pp. 161-168. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol39num2_2010/contentsVol39num2_2010.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Four species of bacteria, Acinetobacter lwoffii, Aeromonas hydrophila, Pseudomonas aeruginosa and Pseudomonas putida, were isolated from soil contaminated with hydrocarbons and selected for the determination of growth requirements and the ability to degrade petroleum hydrocarbon. The bacteria were grown in mineral salt medium (MSM) supplemented with two types of crude oil, either Sumandak or South Angsi at 1% (v/v) concentration. The optimum pH for growth of A. hydrophila and P. aeruginosa was 6.5 when grown with Sumandak and South Angsi oil. For A. lwoffii and P. putida the optimum pH for growth with Sumandak and South Angsi oil was 6.5 or 7.0, respectively. The growth of P. aeruginosa was the highest in MSM when supplemented with 1% South Angsi oil and 0.5% tryptone at pH 6.5 while, in Sumandak oil the growth was the highest when yeast extract was added. Gas chromatography analysis showed that the South Angsi crude oil components of C12 to C25 were more extensively degraded by A. lwoffii after 24 h of incubation compared to the other bacteria over the same period.
format Article
author Ainon Hamzah,
Amir Rabu,
Raja Farzarul Hanim Raja Azmy,
Noor Aini Yussoff,
spellingShingle Ainon Hamzah,
Amir Rabu,
Raja Farzarul Hanim Raja Azmy,
Noor Aini Yussoff,
Isolation and characterization of bacteria degrading Sumandak and South Angsi Oils
author_facet Ainon Hamzah,
Amir Rabu,
Raja Farzarul Hanim Raja Azmy,
Noor Aini Yussoff,
author_sort Ainon Hamzah,
title Isolation and characterization of bacteria degrading Sumandak and South Angsi Oils
title_short Isolation and characterization of bacteria degrading Sumandak and South Angsi Oils
title_full Isolation and characterization of bacteria degrading Sumandak and South Angsi Oils
title_fullStr Isolation and characterization of bacteria degrading Sumandak and South Angsi Oils
title_full_unstemmed Isolation and characterization of bacteria degrading Sumandak and South Angsi Oils
title_sort isolation and characterization of bacteria degrading sumandak and south angsi oils
publisher Universiti Kebangsaan Malaysia
publishDate 2010
url http://journalarticle.ukm.my/7315/1/01_Md_Yeaminhossain.pdf
http://journalarticle.ukm.my/7315/
http://www.ukm.my/jsm/english_journals/vol39num2_2010/contentsVol39num2_2010.html
_version_ 1643737082448838656
score 13.211869

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