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Transposable elements and the detection of somaclonal variation in plant tissue culture

Plant tissue culture uses the genetic potential, or totipotency, of plants to regenerate and give rise to a whole plant. Tissue culture-derived plants are expected to have identical genetic material to the parent, and thus can serve as an effective tool for controlled, mass clonal propagation. Howev...

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Main Authors: Azman, A.S., Mhiri, C.,, Grandbastien, M.A.,, Tam, S.M.,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2014
Online Access:http://journalarticle.ukm.my/7283/1/43_1_01.pdf
http://journalarticle.ukm.my/7283/
http://mabjournal.com/
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spelling my-ukm.journal.72832016-12-14T06:43:37Z http://journalarticle.ukm.my/7283/ Transposable elements and the detection of somaclonal variation in plant tissue culture Azman, A.S. Mhiri, C., Grandbastien, M.A., Tam, S.M., Plant tissue culture uses the genetic potential, or totipotency, of plants to regenerate and give rise to a whole plant. Tissue culture-derived plants are expected to have identical genetic material to the parent, and thus can serve as an effective tool for controlled, mass clonal propagation. However, somaclonal variation has been observed in plant tissue culture due to either point mutations, transposition activity of mobile genetic elements, chromosomal rearrangements, or ploidy level changes, causing genetic instability. Tissue culture-induced mutations associated with transposable element activities have been reported from many plant studies, related to stress conditions during tissue culture such as wounding, exposure to hormones and/or specific compounds in the growth media, and genomic shocks from cytological changes. Transposable elements are repetitive DNA fragments with the ability to transpose from one region to another within a genome. Mutations that may occur during plant tissue culture suggest that the plantlets have to be regularly monitored. Methyl-sensitive transposon display (MSTD) is an advanced, efficient DNA fingerprinting technique that can simultaneously detect genetic variation, changes in transposable element insertion sites and the status of cytosine methylation of DNA in plant genomes. The main principles and notes for application of MSTD such as design of element-specific primers, identification of transposable element sequences in plant genomes, selection of the isochizomer enzymes used, and different classes of banding pattern shown are briefly discussed based on our preliminary work with Nicotiana benthamiana (Tnt1 retroelement) and Musa acuminata (Copia-33 Mad-I retroelement) examples. Penerbit Universiti Kebangsaan Malaysia 2014-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7283/1/43_1_01.pdf Azman, A.S. and Mhiri, C., and Grandbastien, M.A., and Tam, S.M., (2014) Transposable elements and the detection of somaclonal variation in plant tissue culture. Malaysian Applied Biology, 43 (1). pp. 1-12. ISSN 0126-8643 http://mabjournal.com/
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Plant tissue culture uses the genetic potential, or totipotency, of plants to regenerate and give rise to a whole plant. Tissue culture-derived plants are expected to have identical genetic material to the parent, and thus can serve as an effective tool for controlled, mass clonal propagation. However, somaclonal variation has been observed in plant tissue culture due to either point mutations, transposition activity of mobile genetic elements, chromosomal rearrangements, or ploidy level changes, causing genetic instability. Tissue culture-induced mutations associated with transposable element activities have been reported from many plant studies, related to stress conditions during tissue culture such as wounding, exposure to hormones and/or specific compounds in the growth media, and genomic shocks from cytological changes. Transposable elements are repetitive DNA fragments with the ability to transpose from one region to another within a genome. Mutations that may occur during plant tissue culture suggest that the plantlets have to be regularly monitored. Methyl-sensitive transposon display (MSTD) is an advanced, efficient DNA fingerprinting technique that can simultaneously detect genetic variation, changes in transposable element insertion sites and the status of cytosine methylation of DNA in plant genomes. The main principles and notes for application of MSTD such as design of element-specific primers, identification of transposable element sequences in plant genomes, selection of the isochizomer enzymes used, and different classes of banding pattern shown are briefly discussed based on our preliminary work with Nicotiana benthamiana (Tnt1 retroelement) and Musa acuminata (Copia-33 Mad-I retroelement) examples.
format Article
author Azman, A.S.
Mhiri, C.,
Grandbastien, M.A.,
Tam, S.M.,
spellingShingle Azman, A.S.
Mhiri, C.,
Grandbastien, M.A.,
Tam, S.M.,
Transposable elements and the detection of somaclonal variation in plant tissue culture
author_facet Azman, A.S.
Mhiri, C.,
Grandbastien, M.A.,
Tam, S.M.,
author_sort Azman, A.S.
title Transposable elements and the detection of somaclonal variation in plant tissue culture
title_short Transposable elements and the detection of somaclonal variation in plant tissue culture
title_full Transposable elements and the detection of somaclonal variation in plant tissue culture
title_fullStr Transposable elements and the detection of somaclonal variation in plant tissue culture
title_full_unstemmed Transposable elements and the detection of somaclonal variation in plant tissue culture
title_sort transposable elements and the detection of somaclonal variation in plant tissue culture
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2014
url http://journalarticle.ukm.my/7283/1/43_1_01.pdf
http://journalarticle.ukm.my/7283/
http://mabjournal.com/
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score 13.211869

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