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Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1

Cunninghamella bainieri 2A1 is an oleaginous fungus whose lipid accumulation profile is significantly influenced by metal ion concentrations in growth medium. Mg2+, Fe3+, Mn2+ and Cu2+ were found to be the important elements affecting lipid accumulation in this fungus. This study employs a statistic...

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Main Authors: Vidyah Manikan,, Mohd Sahaid Kalil,, Othman Omar,, Abdul Jalil Abdul Kader,, Aidil Abdul Hamid,
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2014
Online Access:http://journalarticle.ukm.my/6931/1/15_Vidyah_Manikan.pdf
http://journalarticle.ukm.my/6931/
http://www.ukm.my/jsm/
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spelling my-ukm.journal.69312016-12-14T06:42:37Z http://journalarticle.ukm.my/6931/ Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1 Vidyah Manikan, Mohd Sahaid Kalil, Othman Omar, Abdul Jalil Abdul Kader, Aidil Abdul Hamid, Cunninghamella bainieri 2A1 is an oleaginous fungus whose lipid accumulation profile is significantly influenced by metal ion concentrations in growth medium. Mg2+, Fe3+, Mn2+ and Cu2+ were found to be the important elements affecting lipid accumulation in this fungus. This study employs a statistical method (Response Surface Methodology – RSM) to study the combined effects of Mg2+, Fe3+, Mn2+ and Cu2+ on lipid accumulation of C. baineri 2A1. Cultivation was carried out in 250 mL Erlenmeyer flasks containing 100 mL nitrogen limited medium at 30ºC and 250 rpm agitation for 120 h. A thirty-run central composite design experiment was employed to identify and optimize the significant factors. In addition to Mg2+ and Fe3+ which were shown to have significant effects on lipid accumulation, the interactions between Mg2+ and Cu2+, as well as the effect of Cu2+ in quadratic terms were also found to have significant effect on the process (p<0.05). The highest amount of lipid obtained in this study was 39% g/g biomass with optimal levels of Mg2+, Fe3+ and Cu2+ at 5.00, 0.017 and 0.0005 g/L, respectively, while Mn2+ was omitted. A 32% increment in lipid yield was recorded, where the lipid content increased to 38%, compared to initial yield of 29% g/g biomass prior to optimization. In conclusion, Mg2+ and Fe3+ have significant positive effect on the lipid accumulation of this fungus, whereas Mn2+ and Cu2+ exert negative effects in combination. Universiti Kebangsaan Malaysia 2014-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6931/1/15_Vidyah_Manikan.pdf Vidyah Manikan, and Mohd Sahaid Kalil, and Othman Omar, and Abdul Jalil Abdul Kader, and Aidil Abdul Hamid, (2014) Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1. Sains Malaysiana, 43 (3). pp. 443-449. ISSN 0126-6039 http://www.ukm.my/jsm/
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Cunninghamella bainieri 2A1 is an oleaginous fungus whose lipid accumulation profile is significantly influenced by metal ion concentrations in growth medium. Mg2+, Fe3+, Mn2+ and Cu2+ were found to be the important elements affecting lipid accumulation in this fungus. This study employs a statistical method (Response Surface Methodology – RSM) to study the combined effects of Mg2+, Fe3+, Mn2+ and Cu2+ on lipid accumulation of C. baineri 2A1. Cultivation was carried out in 250 mL Erlenmeyer flasks containing 100 mL nitrogen limited medium at 30ºC and 250 rpm agitation for 120 h. A thirty-run central composite design experiment was employed to identify and optimize the significant factors. In addition to Mg2+ and Fe3+ which were shown to have significant effects on lipid accumulation, the interactions between Mg2+ and Cu2+, as well as the effect of Cu2+ in quadratic terms were also found to have significant effect on the process (p<0.05). The highest amount of lipid obtained in this study was 39% g/g biomass with optimal levels of Mg2+, Fe3+ and Cu2+ at 5.00, 0.017 and 0.0005 g/L, respectively, while Mn2+ was omitted. A 32% increment in lipid yield was recorded, where the lipid content increased to 38%, compared to initial yield of 29% g/g biomass prior to optimization. In conclusion, Mg2+ and Fe3+ have significant positive effect on the lipid accumulation of this fungus, whereas Mn2+ and Cu2+ exert negative effects in combination.
format Article
author Vidyah Manikan,
Mohd Sahaid Kalil,
Othman Omar,
Abdul Jalil Abdul Kader,
Aidil Abdul Hamid,
spellingShingle Vidyah Manikan,
Mohd Sahaid Kalil,
Othman Omar,
Abdul Jalil Abdul Kader,
Aidil Abdul Hamid,
Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1
author_facet Vidyah Manikan,
Mohd Sahaid Kalil,
Othman Omar,
Abdul Jalil Abdul Kader,
Aidil Abdul Hamid,
author_sort Vidyah Manikan,
title Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1
title_short Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1
title_full Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1
title_fullStr Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1
title_full_unstemmed Effects of Mg2+, Fe3+, Mn2+ and Cu2+ Ions on lipid accumulation by cunninghamella bainieri 2A1
title_sort effects of mg2+, fe3+, mn2+ and cu2+ ions on lipid accumulation by cunninghamella bainieri 2a1
publisher Universiti Kebangsaan Malaysia
publishDate 2014
url http://journalarticle.ukm.my/6931/1/15_Vidyah_Manikan.pdf
http://journalarticle.ukm.my/6931/
http://www.ukm.my/jsm/
_version_ 1643736943510421504
score 13.222552

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