Cover Image

Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells

Salted fish is a locally processed raw food which is used in everyday cooking among Malaysians. Previous studies suggested that salted fish intake was a risk of nasopharyngeal cancer. Hence, this study was carried out to evaluate gene mutation effects through the induction of mutagenic effect of aqu...

Full description

Saved in:
Bibliographic Details
Main Authors: Ahmad Rohi Ghazali,, Rasyidah Mohamad Halim,, Nor Fadilah Rajab,, Noorain Ramli,, Rozaini Abdullah,, Firdaus Kamarulzaman,, Zaliha Harun,
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2013
Online Access:http://journalarticle.ukm.my/6624/1/09_Ahmad_Rohi_Ghazali-1.pdf
http://journalarticle.ukm.my/6624/
http://www.ukm.my/jsm/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-ukm.journal.6624
record_format eprints
spelling my-ukm.journal.66242016-12-14T06:41:43Z http://journalarticle.ukm.my/6624/ Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells Ahmad Rohi Ghazali, Rasyidah Mohamad Halim, Nor Fadilah Rajab, Noorain Ramli, Rozaini Abdullah, Firdaus Kamarulzaman, Zaliha Harun, Salted fish is a locally processed raw food which is used in everyday cooking among Malaysians. Previous studies suggested that salted fish intake was a risk of nasopharyngeal cancer. Hence, this study was carried out to evaluate gene mutation effects through the induction of mutagenic effect of aqueous and methanol extracts of salted fish from Balik Pulau, Pulau Pinang, Malaysia. Balik Pulau was chosen for sampling purpose due to its popularity as a commercial centre for local raw fisheries in Malaysia. Evaluation of mutagenic effect was carried out by hprt Gene Mutation Assay towards V79 lung fibroblast cells. It was found that the aqueous and methanol extracts of salted fish were not cytotoxic towards V79 lung fibroblast cells. It was also found that the extracts of salted fish from Balik Pulau were not mutagenic towards hprt gene of V79 lung fibroblast cells as the mutation frequency of the extracts did not exceed 3 times of the value for negative control mutation frequency. In conclusion, both aqueous and methanol extracts of salted fish from Balik Pulau did not have gene mutation effect towards hprt gene in vitro. However, other toxicological profile could be assessed to determine the mechanism of toxicity of salted fish. Universiti Kebangsaan Malaysia 2013-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6624/1/09_Ahmad_Rohi_Ghazali-1.pdf Ahmad Rohi Ghazali, and Rasyidah Mohamad Halim, and Nor Fadilah Rajab, and Noorain Ramli, and Rozaini Abdullah, and Firdaus Kamarulzaman, and Zaliha Harun, (2013) Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells. Sains Malaysiana, 42 (11). pp. 1599-1603. ISSN 0126-6039 http://www.ukm.my/jsm/
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Salted fish is a locally processed raw food which is used in everyday cooking among Malaysians. Previous studies suggested that salted fish intake was a risk of nasopharyngeal cancer. Hence, this study was carried out to evaluate gene mutation effects through the induction of mutagenic effect of aqueous and methanol extracts of salted fish from Balik Pulau, Pulau Pinang, Malaysia. Balik Pulau was chosen for sampling purpose due to its popularity as a commercial centre for local raw fisheries in Malaysia. Evaluation of mutagenic effect was carried out by hprt Gene Mutation Assay towards V79 lung fibroblast cells. It was found that the aqueous and methanol extracts of salted fish were not cytotoxic towards V79 lung fibroblast cells. It was also found that the extracts of salted fish from Balik Pulau were not mutagenic towards hprt gene of V79 lung fibroblast cells as the mutation frequency of the extracts did not exceed 3 times of the value for negative control mutation frequency. In conclusion, both aqueous and methanol extracts of salted fish from Balik Pulau did not have gene mutation effect towards hprt gene in vitro. However, other toxicological profile could be assessed to determine the mechanism of toxicity of salted fish.
format Article
author Ahmad Rohi Ghazali,
Rasyidah Mohamad Halim,
Nor Fadilah Rajab,
Noorain Ramli,
Rozaini Abdullah,
Firdaus Kamarulzaman,
Zaliha Harun,
spellingShingle Ahmad Rohi Ghazali,
Rasyidah Mohamad Halim,
Nor Fadilah Rajab,
Noorain Ramli,
Rozaini Abdullah,
Firdaus Kamarulzaman,
Zaliha Harun,
Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells
author_facet Ahmad Rohi Ghazali,
Rasyidah Mohamad Halim,
Nor Fadilah Rajab,
Noorain Ramli,
Rozaini Abdullah,
Firdaus Kamarulzaman,
Zaliha Harun,
author_sort Ahmad Rohi Ghazali,
title Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells
title_short Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells
title_full Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells
title_fullStr Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells
title_full_unstemmed Gene mutation effect of aqueous and methanol extracts of salted fish from Pulau Pinang, Malaysia towards V79 lung fibroblast cells
title_sort gene mutation effect of aqueous and methanol extracts of salted fish from pulau pinang, malaysia towards v79 lung fibroblast cells
publisher Universiti Kebangsaan Malaysia
publishDate 2013
url http://journalarticle.ukm.my/6624/1/09_Ahmad_Rohi_Ghazali-1.pdf
http://journalarticle.ukm.my/6624/
http://www.ukm.my/jsm/
_version_ 1643736838479806464
score 13.211869

Similar Items