Cover Image

Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee

An efficient analytical technique capable of analyzing three most common phosphodiesterase-5 (PDE5) inhibitors (vardenafil, sildenafil and tadalafil) simultaneously in premix coffee was developed. Sample extractions using either acetonitrile or methanol with two different extraction techniques (with...

Full description

Saved in:
Bibliographic Details
Main Authors: Zailina, A.M, Aminah, A, Mohd Ambar, Y
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2013
Online Access:http://journalarticle.ukm.my/5895/1/03%2520A.M.%2520Zailina.pdf
http://journalarticle.ukm.my/5895/
http://www.ukm.my/jsm/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-ukm.journal.5895
record_format eprints
spelling my-ukm.journal.58952016-12-14T06:39:47Z http://journalarticle.ukm.my/5895/ Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee Zailina, A.M Aminah, A Mohd Ambar, Y An efficient analytical technique capable of analyzing three most common phosphodiesterase-5 (PDE5) inhibitors (vardenafil, sildenafil and tadalafil) simultaneously in premix coffee was developed. Sample extractions using either acetonitrile or methanol with two different extraction techniques (with and without evaporation steps) were evaluated. Identification and quantitation was conducted by high performance liquid chromatography with photo-diode-array (HPLC-DAD) at different wavelengths; 230 nm, 245 nm and 290 nm; and by time of flight mass spectrometry (LC-MS-TOF). Extraction with acetonitrile (without evaporation with nitrogen) showed recovery ranging from 105% to 113% (± <10%) for HPLC-DAD at 245 nm and 93% to 102% (± <2.5%) for LC-MS-TOF. Chromatogram separation was best achieved with mobile phase consisted of water (0.1% formic acid) and acetonitrile (0.1% formic acid) with gradient elution within 20 min. Thus, the results indicated that extraction using acetonitrile without evaporation step was the most efficient technique for determination of PDE5 inhibitors in premix coffee. Universiti Kebangsaan Malaysia 2013-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/5895/1/03%2520A.M.%2520Zailina.pdf Zailina, A.M and Aminah, A and Mohd Ambar, Y (2013) Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee. Sains Malaysiana, 42 (2). pp. 135-142. ISSN 0126-6039 http://www.ukm.my/jsm/
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description An efficient analytical technique capable of analyzing three most common phosphodiesterase-5 (PDE5) inhibitors (vardenafil, sildenafil and tadalafil) simultaneously in premix coffee was developed. Sample extractions using either acetonitrile or methanol with two different extraction techniques (with and without evaporation steps) were evaluated. Identification and quantitation was conducted by high performance liquid chromatography with photo-diode-array (HPLC-DAD) at different wavelengths; 230 nm, 245 nm and 290 nm; and by time of flight mass spectrometry (LC-MS-TOF). Extraction with acetonitrile (without evaporation with nitrogen) showed recovery ranging from 105% to 113% (± <10%) for HPLC-DAD at 245 nm and 93% to 102% (± <2.5%) for LC-MS-TOF. Chromatogram separation was best achieved with mobile phase consisted of water (0.1% formic acid) and acetonitrile (0.1% formic acid) with gradient elution within 20 min. Thus, the results indicated that extraction using acetonitrile without evaporation step was the most efficient technique for determination of PDE5 inhibitors in premix coffee.
format Article
author Zailina, A.M
Aminah, A
Mohd Ambar, Y
spellingShingle Zailina, A.M
Aminah, A
Mohd Ambar, Y
Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee
author_facet Zailina, A.M
Aminah, A
Mohd Ambar, Y
author_sort Zailina, A.M
title Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee
title_short Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee
title_full Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee
title_fullStr Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee
title_full_unstemmed Optimization of extraction methods for determination of phosphodiesterase-5 (PDE5) inhibitors in premix coffee
title_sort optimization of extraction methods for determination of phosphodiesterase-5 (pde5) inhibitors in premix coffee
publisher Universiti Kebangsaan Malaysia
publishDate 2013
url http://journalarticle.ukm.my/5895/1/03%2520A.M.%2520Zailina.pdf
http://journalarticle.ukm.my/5895/
http://www.ukm.my/jsm/
_version_ 1643736582599999488
score 13.211869

Similar Items