Cover Image

Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens

Bacteriocin or Bacteriocin like inhibitory substances (BLIS) is a protein antibiotic that has a relatively narrow spectrum of killing activity. It could potentially serve as a natural alternative to antibiotics in reducing the development of multi-drug resistant bacteria. Antimicrobial activity of t...

Full description

Saved in:
Bibliographic Details
Main Authors: Azizah Ahmad Fauzi,, Zaleha Shafiei,, Badiah Baharin,, Nurulhuda Mohd,
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2013
Online Access:http://journalarticle.ukm.my/5699/1/04%2520Azizah%2520Ahmad.pdf
http://journalarticle.ukm.my/5699/
http://www.ukm.my/jsm/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-ukm.journal.5699
record_format eprints
spelling my-ukm.journal.56992016-12-14T06:39:14Z http://journalarticle.ukm.my/5699/ Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens Azizah Ahmad Fauzi, Zaleha Shafiei, Badiah Baharin, Nurulhuda Mohd, Bacteriocin or Bacteriocin like inhibitory substances (BLIS) is a protein antibiotic that has a relatively narrow spectrum of killing activity. It could potentially serve as a natural alternative to antibiotics in reducing the development of multi-drug resistant bacteria. Antimicrobial activity of the strains of Lactobacillus sp. isolated from healthy subjects (test strains) against Aggregatibacter actinomycetemcomitans and other periodontal pathogens (indicator strains) isolated from subgingival plaques of aggressive periodontitis patients were determined by using deferred antagonism test and agar-well diffusion method. Strains of Lactobacillus sp., Aggregatibacter actinomycetemcomitans and black pigmented bacteria were selectively isolated from TJA, TSBV and TSBA agars, respectively. Mean diameter zone of inhibition of at least 10 mm was considered as positive results for both methods. Out of 25 strains of Lactobacillus sp. screened, only eight test strains of Lactobacillus sp. showed the specific antimicrobial activity against certain strains of indicator periodontal pathogens during deferred antagonism test. However, out of eight potential strains, only three strains, which were Lactobacillus sp. strain S, Lactobacillus sp. strain V and Lactobacillus sp. strain W consistently showed positive inhibitory activity against black pigmented bacteria by deferred antagonism test and agar-well diffusion method. Therefore, these three strains should be considered as potential BLIS producer strains for further study Universiti Kebangsaan Malaysia 2013-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/5699/1/04%2520Azizah%2520Ahmad.pdf Azizah Ahmad Fauzi, and Zaleha Shafiei, and Badiah Baharin, and Nurulhuda Mohd, (2013) Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens. Sains Malaysiana, 42 (1). pp. 19-24. ISSN 0126-6039 http://www.ukm.my/jsm/
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Bacteriocin or Bacteriocin like inhibitory substances (BLIS) is a protein antibiotic that has a relatively narrow spectrum of killing activity. It could potentially serve as a natural alternative to antibiotics in reducing the development of multi-drug resistant bacteria. Antimicrobial activity of the strains of Lactobacillus sp. isolated from healthy subjects (test strains) against Aggregatibacter actinomycetemcomitans and other periodontal pathogens (indicator strains) isolated from subgingival plaques of aggressive periodontitis patients were determined by using deferred antagonism test and agar-well diffusion method. Strains of Lactobacillus sp., Aggregatibacter actinomycetemcomitans and black pigmented bacteria were selectively isolated from TJA, TSBV and TSBA agars, respectively. Mean diameter zone of inhibition of at least 10 mm was considered as positive results for both methods. Out of 25 strains of Lactobacillus sp. screened, only eight test strains of Lactobacillus sp. showed the specific antimicrobial activity against certain strains of indicator periodontal pathogens during deferred antagonism test. However, out of eight potential strains, only three strains, which were Lactobacillus sp. strain S, Lactobacillus sp. strain V and Lactobacillus sp. strain W consistently showed positive inhibitory activity against black pigmented bacteria by deferred antagonism test and agar-well diffusion method. Therefore, these three strains should be considered as potential BLIS producer strains for further study
format Article
author Azizah Ahmad Fauzi,
Zaleha Shafiei,
Badiah Baharin,
Nurulhuda Mohd,
spellingShingle Azizah Ahmad Fauzi,
Zaleha Shafiei,
Badiah Baharin,
Nurulhuda Mohd,
Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens
author_facet Azizah Ahmad Fauzi,
Zaleha Shafiei,
Badiah Baharin,
Nurulhuda Mohd,
author_sort Azizah Ahmad Fauzi,
title Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens
title_short Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens
title_full Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens
title_fullStr Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens
title_full_unstemmed Isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens
title_sort isolation of lactobacillus from periodontally healthy subjects and its antimicrobial activity against periodontal pathogens
publisher Universiti Kebangsaan Malaysia
publishDate 2013
url http://journalarticle.ukm.my/5699/1/04%2520Azizah%2520Ahmad.pdf
http://journalarticle.ukm.my/5699/
http://www.ukm.my/jsm/
_version_ 1643736506613891072
score 13.211869

Similar Items