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Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l

We report here the isolation and sequence analysis of a cDNA fragment for an endoglucanase gene from Aspergillus terreus SUK-I. A pair of primers was designed based on conserved regions of endoglucanase gene. Amplification of A. terreus SUK-I cDNA using the primers produced a DNA band of 642 bp. The...

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Main Authors: Nik Marzuki Sidik,, Shaiful Adzni Sharifuddin,, Othman Omar ,, Abdul Jalil Abdul Kader,, Sahidan Senafi,
Format: Article
Published: Universiti Kebangsaan Malaysia 2006
Online Access:http://journalarticle.ukm.my/3971/
http://www.ukm.my/jsm/english_journals/vol35num1_2006/vol35num1_06page31-35.html
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spelling my-ukm.journal.39712012-03-28T00:53:11Z http://journalarticle.ukm.my/3971/ Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l Nik Marzuki Sidik, Shaiful Adzni Sharifuddin, Othman Omar , Abdul Jalil Abdul Kader, Sahidan Senafi, We report here the isolation and sequence analysis of a cDNA fragment for an endoglucanase gene from Aspergillus terreus SUK-I. A pair of primers was designed based on conserved regions of endoglucanase gene. Amplification of A. terreus SUK-I cDNA using the primers produced a DNA band of 642 bp. The cDNA fragment was purified and cloned into pCR®ll-TOPO plasmid vector. The cDNA insert was designated as SA2 and sequenced. Analysis of the SA2 sequence showed a high degree of identity towards endoglucanase gene sequences from A. aculeatus, A kawachii and Talaromyces emersonii. While, the putative amino acid sequence of SA2 showed 74 % identity towards endoglucanase protein from Thermoascus aurantiacus and 69 % identity towards endoglucanase protein from A. kawachii, A. niger, A. aculeatus and A. nidulans. A 66 % identity between SA2 putative amino acid sequence and A. oryzae endoglucanase protein sequence was also observed. Therefore, we concluded that SA2 codes for a putative endoglucanase gene of A. terreus SUK-I. Universiti Kebangsaan Malaysia 2006-07 Article PeerReviewed Nik Marzuki Sidik, and Shaiful Adzni Sharifuddin, and Othman Omar , and Abdul Jalil Abdul Kader, and Sahidan Senafi, (2006) Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l. Sains Malaysiana, 35 (1). pp. 31-35. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol35num1_2006/vol35num1_06page31-35.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
description We report here the isolation and sequence analysis of a cDNA fragment for an endoglucanase gene from Aspergillus terreus SUK-I. A pair of primers was designed based on conserved regions of endoglucanase gene. Amplification of A. terreus SUK-I cDNA using the primers produced a DNA band of 642 bp. The cDNA fragment was purified and cloned into pCR®ll-TOPO plasmid vector. The cDNA insert was designated as SA2 and sequenced. Analysis of the SA2 sequence showed a high degree of identity towards endoglucanase gene sequences from A. aculeatus, A kawachii and Talaromyces emersonii. While, the putative amino acid sequence of SA2 showed 74 % identity towards endoglucanase protein from Thermoascus aurantiacus and 69 % identity towards endoglucanase protein from A. kawachii, A. niger, A. aculeatus and A. nidulans. A 66 % identity between SA2 putative amino acid sequence and A. oryzae endoglucanase protein sequence was also observed. Therefore, we concluded that SA2 codes for a putative endoglucanase gene of A. terreus SUK-I.
format Article
author Nik Marzuki Sidik,
Shaiful Adzni Sharifuddin,
Othman Omar ,
Abdul Jalil Abdul Kader,
Sahidan Senafi,
spellingShingle Nik Marzuki Sidik,
Shaiful Adzni Sharifuddin,
Othman Omar ,
Abdul Jalil Abdul Kader,
Sahidan Senafi,
Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l
author_facet Nik Marzuki Sidik,
Shaiful Adzni Sharifuddin,
Othman Omar ,
Abdul Jalil Abdul Kader,
Sahidan Senafi,
author_sort Nik Marzuki Sidik,
title Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l
title_short Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l
title_full Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l
title_fullStr Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l
title_full_unstemmed Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l
title_sort isolation and molecular analysis of cdna encoding a partial endoglucanase gene from aspergillus terreus suk-l
publisher Universiti Kebangsaan Malaysia
publishDate 2006
url http://journalarticle.ukm.my/3971/
http://www.ukm.my/jsm/english_journals/vol35num1_2006/vol35num1_06page31-35.html
_version_ 1643735915819958272
score 13.211869

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