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Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization

Acanthamoeba is a free living protozoa that can cause keratitis and granulomatous amoebic encephalitis. Physiological characteristics of this amoeba are found to have a medical importance in which it can be related to the pathogenicity potential of the organism. This study was carried out to investi...

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Main Authors: Nurul Farhana Jufri,, Anisah Nordin,, Mohamed Kamel Abd Ghani,, Yusof Suboh,, Noraina Abd Rahim,
Format: Article
Language:English
Published: Penerbit UKM 2011
Online Access:http://journalarticle.ukm.my/3596/1/Survivability_of_Acanthamoeba_Strains_Isolated_from_Clinical_and.pdf
http://journalarticle.ukm.my/3596/
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spelling my-ukm.journal.35962016-12-14T06:34:54Z http://journalarticle.ukm.my/3596/ Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization Nurul Farhana Jufri, Anisah Nordin, Mohamed Kamel Abd Ghani, Yusof Suboh, Noraina Abd Rahim, Acanthamoeba is a free living protozoa that can cause keratitis and granulomatous amoebic encephalitis. Physiological characteristics of this amoeba are found to have a medical importance in which it can be related to the pathogenicity potential of the organism. This study was carried out to investigate the physiological characteristics of survivability during axenization. Six Acanthamoeba strains from three clinical isolates (HSB 1, HKL 48 and HKL 95) and three environmental isolates (PHS 2, PHS 11 and PHS 15) were used in this study. Axenization test was done by treating cysts with hydrochloric acid (3%) and Page saline containing Gentamicin (100 μg/ml). Cysts were then cultured into PYG enrich media, incubated at 30oC and the presence and proliferation of trophozoites of Acanthamoeba were observed. This study showed that PHS 15, HSB 1, HKL 48 and HKL 95 could be axenized but they have poor proliferation rate in PYG enrich media. The result showed that the difference between both clinical and environmental isolates was observed in two strains; PHS 2 and PHS 11. This indicates that there is a possibility that the physiological traits of strains from both isolates are the same and strains from the environment are able to show the pathogenic potential and capable of causing infection to human. Penerbit UKM 2011 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/3596/1/Survivability_of_Acanthamoeba_Strains_Isolated_from_Clinical_and.pdf Nurul Farhana Jufri, and Anisah Nordin, and Mohamed Kamel Abd Ghani, and Yusof Suboh, and Noraina Abd Rahim, (2011) Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization. Jurnal Sains Kesihatan Malaysia, 9 (1). pp. 1-3. ISSN 1675-8161
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Acanthamoeba is a free living protozoa that can cause keratitis and granulomatous amoebic encephalitis. Physiological characteristics of this amoeba are found to have a medical importance in which it can be related to the pathogenicity potential of the organism. This study was carried out to investigate the physiological characteristics of survivability during axenization. Six Acanthamoeba strains from three clinical isolates (HSB 1, HKL 48 and HKL 95) and three environmental isolates (PHS 2, PHS 11 and PHS 15) were used in this study. Axenization test was done by treating cysts with hydrochloric acid (3%) and Page saline containing Gentamicin (100 μg/ml). Cysts were then cultured into PYG enrich media, incubated at 30oC and the presence and proliferation of trophozoites of Acanthamoeba were observed. This study showed that PHS 15, HSB 1, HKL 48 and HKL 95 could be axenized but they have poor proliferation rate in PYG enrich media. The result showed that the difference between both clinical and environmental isolates was observed in two strains; PHS 2 and PHS 11. This indicates that there is a possibility that the physiological traits of strains from both isolates are the same and strains from the environment are able to show the pathogenic potential and capable of causing infection to human.
format Article
author Nurul Farhana Jufri,
Anisah Nordin,
Mohamed Kamel Abd Ghani,
Yusof Suboh,
Noraina Abd Rahim,
spellingShingle Nurul Farhana Jufri,
Anisah Nordin,
Mohamed Kamel Abd Ghani,
Yusof Suboh,
Noraina Abd Rahim,
Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization
author_facet Nurul Farhana Jufri,
Anisah Nordin,
Mohamed Kamel Abd Ghani,
Yusof Suboh,
Noraina Abd Rahim,
author_sort Nurul Farhana Jufri,
title Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization
title_short Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization
title_full Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization
title_fullStr Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization
title_full_unstemmed Survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization
title_sort survivability of acanthamoeba strains isolated from clinical and environmental specimens during axenization
publisher Penerbit UKM
publishDate 2011
url http://journalarticle.ukm.my/3596/1/Survivability_of_Acanthamoeba_Strains_Isolated_from_Clinical_and.pdf
http://journalarticle.ukm.my/3596/
_version_ 1643735780738203648
score 13.211869

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