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Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures

Tocopherols and tocotrienols have been shown in previous studies to protect neurons from oxidative injuries, especially from hydrogen peroxide (H2O2) and buthionine sulfoximine (BSO) induced oxidative stress. In this study, we compared two vitamin E isomers, γ-tocotrienol (GTT) and α-tocopherol (ATF...

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Main Authors: Then, Sue-Mian, Wan Zurinah Wan Ngah,, Gapor Mat Top,, Musalmah Mazlan,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2010
Online Access:http://journalarticle.ukm.my/338/1/22.pdf
http://journalarticle.ukm.my/338/
https://www.ukm.my/jsm/malay_journals/jilid39bil1_2010/KandunganJilid39Bil1_2010.html
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spelling my-ukm.journal.3382022-07-28T04:23:16Z http://journalarticle.ukm.my/338/ Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures Then, Sue-Mian Wan Zurinah Wan Ngah, Gapor Mat Top, Musalmah Mazlan, Tocopherols and tocotrienols have been shown in previous studies to protect neurons from oxidative injuries, especially from hydrogen peroxide (H2O2) and buthionine sulfoximine (BSO) induced oxidative stress. In this study, we compared two vitamin E isomers, γ-tocotrienol (GTT) and α-tocopherol (ATF) in their neuroprotective effects against H2O2-induced apoptosis in primary rat cortical neurons and human neuroblastoma cell line SH-SY5Y. Cytotoxicity screening of H2O2, GTT and ATF was done to determine the IC50 levels. To screen for neuroprotective effects, cortical neurons and SH-SY5Y cell cultures were pre-incubated with GTT or ATF, respectively at different concentrations for 1 hour before concurrent treatment of H2O2 at IC50. Results of these treatments were compared to cells treated with H2O2 only and control cells. Cytotoxicology screening showed that IC50 of H2O2 for cortical neuron is at 50 μM while SH-SY5Y have higher IC50 of 100 μM. GTT is cytotoxic to cortical neurons at ≥50 μM and SH-SY5Y at ≥100 μM while ATF did not show any toxicity within the range of concentration tested (1-750 μM). Results from neuroprotection screening showed that GTT and ATF were able to protect both cortical neurons and SH-SY5Y from H2O2-induced oxidative stress at concetration of ≤10 μM. Cellular uptake of GTT is higher in both cortical neurons and SH-SY5Y as compared to ATF when both cortical neuron and SH-SY5Y were incubated with 10 μM GTT or ATF, respectively for 24 hour. Although primary rat cortical neurons and human neuroblastoma SH-SY5Y were different culture system, the effects of GTT and ATF are similar in both H2O2 –induced culture which strongly suggest that both GTT and ATF act as free radical scavenger to exert their neuroprotective effects. Penerbit Universiti Kebangsaan Malaysia 2010-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/338/1/22.pdf Then, Sue-Mian and Wan Zurinah Wan Ngah, and Gapor Mat Top, and Musalmah Mazlan, (2010) Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures. Sains Malaysiana, 39 (1). pp. 145-156. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid39bil1_2010/KandunganJilid39Bil1_2010.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Tocopherols and tocotrienols have been shown in previous studies to protect neurons from oxidative injuries, especially from hydrogen peroxide (H2O2) and buthionine sulfoximine (BSO) induced oxidative stress. In this study, we compared two vitamin E isomers, γ-tocotrienol (GTT) and α-tocopherol (ATF) in their neuroprotective effects against H2O2-induced apoptosis in primary rat cortical neurons and human neuroblastoma cell line SH-SY5Y. Cytotoxicity screening of H2O2, GTT and ATF was done to determine the IC50 levels. To screen for neuroprotective effects, cortical neurons and SH-SY5Y cell cultures were pre-incubated with GTT or ATF, respectively at different concentrations for 1 hour before concurrent treatment of H2O2 at IC50. Results of these treatments were compared to cells treated with H2O2 only and control cells. Cytotoxicology screening showed that IC50 of H2O2 for cortical neuron is at 50 μM while SH-SY5Y have higher IC50 of 100 μM. GTT is cytotoxic to cortical neurons at ≥50 μM and SH-SY5Y at ≥100 μM while ATF did not show any toxicity within the range of concentration tested (1-750 μM). Results from neuroprotection screening showed that GTT and ATF were able to protect both cortical neurons and SH-SY5Y from H2O2-induced oxidative stress at concetration of ≤10 μM. Cellular uptake of GTT is higher in both cortical neurons and SH-SY5Y as compared to ATF when both cortical neuron and SH-SY5Y were incubated with 10 μM GTT or ATF, respectively for 24 hour. Although primary rat cortical neurons and human neuroblastoma SH-SY5Y were different culture system, the effects of GTT and ATF are similar in both H2O2 –induced culture which strongly suggest that both GTT and ATF act as free radical scavenger to exert their neuroprotective effects.
format Article
author Then, Sue-Mian
Wan Zurinah Wan Ngah,
Gapor Mat Top,
Musalmah Mazlan,
spellingShingle Then, Sue-Mian
Wan Zurinah Wan Ngah,
Gapor Mat Top,
Musalmah Mazlan,
Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures
author_facet Then, Sue-Mian
Wan Zurinah Wan Ngah,
Gapor Mat Top,
Musalmah Mazlan,
author_sort Then, Sue-Mian
title Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures
title_short Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures
title_full Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures
title_fullStr Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures
title_full_unstemmed Comparison of the effects of α-Tocopherol and γ-Tocotrienol against oxidative stress in two different neuronal cultures
title_sort comparison of the effects of α-tocopherol and γ-tocotrienol against oxidative stress in two different neuronal cultures
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2010
url http://journalarticle.ukm.my/338/1/22.pdf
http://journalarticle.ukm.my/338/
https://www.ukm.my/jsm/malay_journals/jilid39bil1_2010/KandunganJilid39Bil1_2010.html
_version_ 1739829183271403520
score 13.211869

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