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Effect of auxins on growth enhancement of cell suspension culture of Tongkat Ali Hitam (Polyalthia bullata)

Polyalthia bullata, a Southeast Asian plant, is valued for its bioactive compounds with pharmaceutical potential. To prevent overharvesting and extinction, cell suspension culture offers a sustainable method for the mass production of these compounds. Despite its effectiveness, no studies on Polyalt...

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Main Authors: Nurul Farhana Farezol,, Munirah Adibah Kamarul Zaman,, Azzreena Mohamad Azzeme,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2024
Online Access:http://journalarticle.ukm.my/24836/1/MAL%204.pdf
http://journalarticle.ukm.my/24836/
https://jms.mabjournal.com/index.php/mab/issue/view/64
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spelling my-ukm.journal.248362025-02-10T07:36:27Z http://journalarticle.ukm.my/24836/ Effect of auxins on growth enhancement of cell suspension culture of Tongkat Ali Hitam (Polyalthia bullata) Nurul Farhana Farezol, Munirah Adibah Kamarul Zaman, Azzreena Mohamad Azzeme, Polyalthia bullata, a Southeast Asian plant, is valued for its bioactive compounds with pharmaceutical potential. To prevent overharvesting and extinction, cell suspension culture offers a sustainable method for the mass production of these compounds. Despite its effectiveness, no studies on Polyalthia bullata cell suspension culture have been established. Therefore, this study aimed to establish the culture by evaluating growth and biomass production. To achieve the objective, leaf derived callus of Polyalthia bullata was multiplied on Murashige and Skoog (MS) + 30 μM dicamba medium. Subsequently, cell suspension initiation and multiplication were carried out using half-strength MS basal medium (½ MSO) supplemented with 5, 15, 25, and 30 μM of 1-naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA), respectively. In this study, suspension cells in the ½ MSO recorded the highest increment in fresh (4.455 ± 1.170 g FW) and dry weight (0.220 ± 0.033 g DW) but produced dark brown cells. Meanwhile, cells grown on ½ MS medium supplemented with 30 μM NAA recorded the highest increase in fresh weight (3.472 ± 0.694 g FW) and dry weight (0.190 ± 0.012 g DW), displaying a light yellowish-brown cell. Although the ½ MSO yielded the highest biomass, the cell suspension cultures supplemented with 30 μM NAA showed promising results, achieving higher biomass compared to other auxin treatments and exhibiting a light yellowish-brown cell. This suggests that 30 μM NAA is a more efficient auxin utilization in reducing the occurrence of dark brown cells. In conclusion, optimizing auxin concentrations is crucial for high-quality Polyalthia bullata cell suspension culture. This study can provide insight into sustainable cultivation practices for the plant, serving as a potential bio-factory for mass-producing bioactive compounds. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24836/1/MAL%204.pdf Nurul Farhana Farezol, and Munirah Adibah Kamarul Zaman, and Azzreena Mohamad Azzeme, (2024) Effect of auxins on growth enhancement of cell suspension culture of Tongkat Ali Hitam (Polyalthia bullata). Malaysian Applied Biology, 53 (5). pp. 49-61. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/issue/view/64
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Polyalthia bullata, a Southeast Asian plant, is valued for its bioactive compounds with pharmaceutical potential. To prevent overharvesting and extinction, cell suspension culture offers a sustainable method for the mass production of these compounds. Despite its effectiveness, no studies on Polyalthia bullata cell suspension culture have been established. Therefore, this study aimed to establish the culture by evaluating growth and biomass production. To achieve the objective, leaf derived callus of Polyalthia bullata was multiplied on Murashige and Skoog (MS) + 30 μM dicamba medium. Subsequently, cell suspension initiation and multiplication were carried out using half-strength MS basal medium (½ MSO) supplemented with 5, 15, 25, and 30 μM of 1-naphthaleneacetic acid (NAA), indole-3-acetic acid (IAA), and indole-3-butyric acid (IBA), respectively. In this study, suspension cells in the ½ MSO recorded the highest increment in fresh (4.455 ± 1.170 g FW) and dry weight (0.220 ± 0.033 g DW) but produced dark brown cells. Meanwhile, cells grown on ½ MS medium supplemented with 30 μM NAA recorded the highest increase in fresh weight (3.472 ± 0.694 g FW) and dry weight (0.190 ± 0.012 g DW), displaying a light yellowish-brown cell. Although the ½ MSO yielded the highest biomass, the cell suspension cultures supplemented with 30 μM NAA showed promising results, achieving higher biomass compared to other auxin treatments and exhibiting a light yellowish-brown cell. This suggests that 30 μM NAA is a more efficient auxin utilization in reducing the occurrence of dark brown cells. In conclusion, optimizing auxin concentrations is crucial for high-quality Polyalthia bullata cell suspension culture. This study can provide insight into sustainable cultivation practices for the plant, serving as a potential bio-factory for mass-producing bioactive compounds.
format Article
author Nurul Farhana Farezol,
Munirah Adibah Kamarul Zaman,
Azzreena Mohamad Azzeme,
spellingShingle Nurul Farhana Farezol,
Munirah Adibah Kamarul Zaman,
Azzreena Mohamad Azzeme,
Effect of auxins on growth enhancement of cell suspension culture of Tongkat Ali Hitam (Polyalthia bullata)
author_facet Nurul Farhana Farezol,
Munirah Adibah Kamarul Zaman,
Azzreena Mohamad Azzeme,
author_sort Nurul Farhana Farezol,
title Effect of auxins on growth enhancement of cell suspension culture of Tongkat Ali Hitam (Polyalthia bullata)
title_short Effect of auxins on growth enhancement of cell suspension culture of Tongkat Ali Hitam (Polyalthia bullata)
title_full Effect of auxins on growth enhancement of cell suspension culture of Tongkat Ali Hitam (Polyalthia bullata)
title_fullStr Effect of auxins on growth enhancement of cell suspension culture of Tongkat Ali Hitam (Polyalthia bullata)
title_full_unstemmed Effect of auxins on growth enhancement of cell suspension culture of Tongkat Ali Hitam (Polyalthia bullata)
title_sort effect of auxins on growth enhancement of cell suspension culture of tongkat ali hitam (polyalthia bullata)
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2024
url http://journalarticle.ukm.my/24836/1/MAL%204.pdf
http://journalarticle.ukm.my/24836/
https://jms.mabjournal.com/index.php/mab/issue/view/64
_version_ 1825161543640154112
score 13.251813

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