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Surface metabolite amino acids and collagen profiling in refrigerated tilapia fish (Oreochromis niloticus): implications for identification and quality assessment

Nile tilapia (Oreochromis niloticus) by-products, including the scales, skin, and mucus, are rich sources of protein with a balanced amino acid profile and high collagen content. Cold storage at 4 ℃ is commonly used to maintain the freshness of tilapia fish in retail settings. This study aims to inv...

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Main Authors: Nuraisah Rabu,, Saiful Irwan Zubairi,, Zainun Nurzahim,, Harisun Yaakob,, Ikhwan Zakaria,, Siti Noor Diana Mohd Kamaruddin,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2024
Online Access:http://journalarticle.ukm.my/24804/1/SMS%2011.pdf
http://journalarticle.ukm.my/24804/
https://www.ukm.my/jsm/english_journals/vol53num10_2024/contentsVol53num10_2024.html
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spelling my-ukm.journal.248042025-02-07T07:52:34Z http://journalarticle.ukm.my/24804/ Surface metabolite amino acids and collagen profiling in refrigerated tilapia fish (Oreochromis niloticus): implications for identification and quality assessment Nuraisah Rabu, Saiful Irwan Zubairi, Zainun Nurzahim, Harisun Yaakob, Ikhwan Zakaria, Siti Noor Diana Mohd Kamaruddin, Nile tilapia (Oreochromis niloticus) by-products, including the scales, skin, and mucus, are rich sources of protein with a balanced amino acid profile and high collagen content. Cold storage at 4 ℃ is commonly used to maintain the freshness of tilapia fish in retail settings. This study aims to investigate the changes in surface metabolites and collagen content in tilapia fish during cold storage at 4 ℃. Fresh tilapia fish was stored at 4 ℃ for 7 days to monitor physicochemical and metabolite changes. Fish collagen content was extracted using acetic acid and pepsin, pH values were measured using a calibrated pH meter, protein content was determined via the Bradford method, and creatine and phenylalanine levels were assessed using High-Performance Liquid Chromatography (HPLC). Analysis was conducted on days 0, 3, and 7 of cold storage. Significant reductions (p<0.05) in collagen content from fish scales were observed on the 3rd day of storage, declining from 72.60 ± 12.40% to 30.17 ± 17.62%. pH levels of fish scales and mucus showed a slight alkaline shift, while the skin turned acidic due to bacterial and enzymatic activities. Protein content in the scales, skin, and mucus showed a substantial loss exceeding 50% after 7 days of cold storage. Changes in creatine and phenylalanine concentrations in the mucus further indicated a decline in tilapia fish freshness due to biochemical reactions post-mortem, compromising overall quality. In conclusion, the duration of cold storage significantly affects the composition of tilapia fish scales, skin, and mucus, with a 7-day storage period identified as a suitable freshness indicator in compliance with FDA guidelines permitting fresh fish to be stored at 4 °C for up to two days. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24804/1/SMS%2011.pdf Nuraisah Rabu, and Saiful Irwan Zubairi, and Zainun Nurzahim, and Harisun Yaakob, and Ikhwan Zakaria, and Siti Noor Diana Mohd Kamaruddin, (2024) Surface metabolite amino acids and collagen profiling in refrigerated tilapia fish (Oreochromis niloticus): implications for identification and quality assessment. Sains Malaysiana, 53 (10). pp. 3369-3381. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num10_2024/contentsVol53num10_2024.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Nile tilapia (Oreochromis niloticus) by-products, including the scales, skin, and mucus, are rich sources of protein with a balanced amino acid profile and high collagen content. Cold storage at 4 ℃ is commonly used to maintain the freshness of tilapia fish in retail settings. This study aims to investigate the changes in surface metabolites and collagen content in tilapia fish during cold storage at 4 ℃. Fresh tilapia fish was stored at 4 ℃ for 7 days to monitor physicochemical and metabolite changes. Fish collagen content was extracted using acetic acid and pepsin, pH values were measured using a calibrated pH meter, protein content was determined via the Bradford method, and creatine and phenylalanine levels were assessed using High-Performance Liquid Chromatography (HPLC). Analysis was conducted on days 0, 3, and 7 of cold storage. Significant reductions (p<0.05) in collagen content from fish scales were observed on the 3rd day of storage, declining from 72.60 ± 12.40% to 30.17 ± 17.62%. pH levels of fish scales and mucus showed a slight alkaline shift, while the skin turned acidic due to bacterial and enzymatic activities. Protein content in the scales, skin, and mucus showed a substantial loss exceeding 50% after 7 days of cold storage. Changes in creatine and phenylalanine concentrations in the mucus further indicated a decline in tilapia fish freshness due to biochemical reactions post-mortem, compromising overall quality. In conclusion, the duration of cold storage significantly affects the composition of tilapia fish scales, skin, and mucus, with a 7-day storage period identified as a suitable freshness indicator in compliance with FDA guidelines permitting fresh fish to be stored at 4 °C for up to two days.
format Article
author Nuraisah Rabu,
Saiful Irwan Zubairi,
Zainun Nurzahim,
Harisun Yaakob,
Ikhwan Zakaria,
Siti Noor Diana Mohd Kamaruddin,
spellingShingle Nuraisah Rabu,
Saiful Irwan Zubairi,
Zainun Nurzahim,
Harisun Yaakob,
Ikhwan Zakaria,
Siti Noor Diana Mohd Kamaruddin,
Surface metabolite amino acids and collagen profiling in refrigerated tilapia fish (Oreochromis niloticus): implications for identification and quality assessment
author_facet Nuraisah Rabu,
Saiful Irwan Zubairi,
Zainun Nurzahim,
Harisun Yaakob,
Ikhwan Zakaria,
Siti Noor Diana Mohd Kamaruddin,
author_sort Nuraisah Rabu,
title Surface metabolite amino acids and collagen profiling in refrigerated tilapia fish (Oreochromis niloticus): implications for identification and quality assessment
title_short Surface metabolite amino acids and collagen profiling in refrigerated tilapia fish (Oreochromis niloticus): implications for identification and quality assessment
title_full Surface metabolite amino acids and collagen profiling in refrigerated tilapia fish (Oreochromis niloticus): implications for identification and quality assessment
title_fullStr Surface metabolite amino acids and collagen profiling in refrigerated tilapia fish (Oreochromis niloticus): implications for identification and quality assessment
title_full_unstemmed Surface metabolite amino acids and collagen profiling in refrigerated tilapia fish (Oreochromis niloticus): implications for identification and quality assessment
title_sort surface metabolite amino acids and collagen profiling in refrigerated tilapia fish (oreochromis niloticus): implications for identification and quality assessment
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2024
url http://journalarticle.ukm.my/24804/1/SMS%2011.pdf
http://journalarticle.ukm.my/24804/
https://www.ukm.my/jsm/english_journals/vol53num10_2024/contentsVol53num10_2024.html
_version_ 1823533642133012480
score 13.244413

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