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Essential oil with mosquito-repellent potential from plectranthus amboinicus leaves against aedes aegypti via human volunteer study

Plectranthus amboinicus (PA) is a highly aromatic perennial shrub that belongs to the Lamiaceae family. It has thick, f leshy and succulent leaves and stem. The margins of the leaves contain numerous glandular hairs that produce essential oil. The essential oil of PA(PAEO) exhibits therapeutical, nu...

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Main Authors: Rizafizah Othaman,, Sumathy Raghavan,, Rozida Mohd Khalid,, Nabhan Sharer,, Muntaz Abu Bakar,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2024
Online Access:http://journalarticle.ukm.my/24483/1/SS%201.pdf
http://journalarticle.ukm.my/24483/
https://www.ukm.my/jsm/english_journals/vol53num9_2024/contentsVol53num9_2024.html
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spelling my-ukm.journal.244832024-11-12T02:09:13Z http://journalarticle.ukm.my/24483/ Essential oil with mosquito-repellent potential from plectranthus amboinicus leaves against aedes aegypti via human volunteer study Rizafizah Othaman, Sumathy Raghavan, Rozida Mohd Khalid, Nabhan Sharer, Muntaz Abu Bakar, Plectranthus amboinicus (PA) is a highly aromatic perennial shrub that belongs to the Lamiaceae family. It has thick, f leshy and succulent leaves and stem. The margins of the leaves contain numerous glandular hairs that produce essential oil. The essential oil of PA(PAEO) exhibits therapeutical, nutritional, antioxidant, antimicrobial, insecticidal, and insect repellent potentials. The objective of this study was to evaluate and compare the repellent properties of PAEO and DEET against Aedes aegypti mosquitoes, which are known carriers of dengue fever. The extraction of PAEO was carried out using hydrodistillation method while the chemical profile was analysed by gas chromatography-mass spectrometry (GC-MS) technique. The bioefficacy test was conducted according to the household insecticide products personal mosquito repellent evaluation method for biological efficacy (First revision) (MS1497:2007) provided by the Standard and Industrial Research Institute of Malaysia (SIRIM) with slight modification. 10% PAEO was used as test solution while 10% DEET was used as positive control. Fabric and patches treated with 10% PAEO were tested on human volunteers. Both the fabric and the patch, treated with 10% PAEO, exhibit excellent repellency (97 - 100%) against Ae. aegypti mosquitoes for a duration of up to 240 min (4 h) post-application. In contrast, 10% DEET, which initially showed a comparable repellency (99%) 30 min after application, experiences a gradual decline in repellency over time, reaching a 51% repellency rate at the end of the test (240 min post-application). A two-way ANOVA analysis indicates significant effect for type of treatment (p = 0.016) but with no significant difference between the exposure time. Tukey’s test showed significant differences between 10% PAEO-treated patches and 10% DEET (p = 0.016), and between 10% PAEO-treated fabric and 10% DEET (p = 0.026). These findings suggest that PAEO is potentially an effective natural repellent against Ae. aegypti. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24483/1/SS%201.pdf Rizafizah Othaman, and Sumathy Raghavan, and Rozida Mohd Khalid, and Nabhan Sharer, and Muntaz Abu Bakar, (2024) Essential oil with mosquito-repellent potential from plectranthus amboinicus leaves against aedes aegypti via human volunteer study. Sains Malaysiana, 53 (9). pp. 2021-2032. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num9_2024/contentsVol53num9_2024.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Plectranthus amboinicus (PA) is a highly aromatic perennial shrub that belongs to the Lamiaceae family. It has thick, f leshy and succulent leaves and stem. The margins of the leaves contain numerous glandular hairs that produce essential oil. The essential oil of PA(PAEO) exhibits therapeutical, nutritional, antioxidant, antimicrobial, insecticidal, and insect repellent potentials. The objective of this study was to evaluate and compare the repellent properties of PAEO and DEET against Aedes aegypti mosquitoes, which are known carriers of dengue fever. The extraction of PAEO was carried out using hydrodistillation method while the chemical profile was analysed by gas chromatography-mass spectrometry (GC-MS) technique. The bioefficacy test was conducted according to the household insecticide products personal mosquito repellent evaluation method for biological efficacy (First revision) (MS1497:2007) provided by the Standard and Industrial Research Institute of Malaysia (SIRIM) with slight modification. 10% PAEO was used as test solution while 10% DEET was used as positive control. Fabric and patches treated with 10% PAEO were tested on human volunteers. Both the fabric and the patch, treated with 10% PAEO, exhibit excellent repellency (97 - 100%) against Ae. aegypti mosquitoes for a duration of up to 240 min (4 h) post-application. In contrast, 10% DEET, which initially showed a comparable repellency (99%) 30 min after application, experiences a gradual decline in repellency over time, reaching a 51% repellency rate at the end of the test (240 min post-application). A two-way ANOVA analysis indicates significant effect for type of treatment (p = 0.016) but with no significant difference between the exposure time. Tukey’s test showed significant differences between 10% PAEO-treated patches and 10% DEET (p = 0.016), and between 10% PAEO-treated fabric and 10% DEET (p = 0.026). These findings suggest that PAEO is potentially an effective natural repellent against Ae. aegypti.
format Article
author Rizafizah Othaman,
Sumathy Raghavan,
Rozida Mohd Khalid,
Nabhan Sharer,
Muntaz Abu Bakar,
spellingShingle Rizafizah Othaman,
Sumathy Raghavan,
Rozida Mohd Khalid,
Nabhan Sharer,
Muntaz Abu Bakar,
Essential oil with mosquito-repellent potential from plectranthus amboinicus leaves against aedes aegypti via human volunteer study
author_facet Rizafizah Othaman,
Sumathy Raghavan,
Rozida Mohd Khalid,
Nabhan Sharer,
Muntaz Abu Bakar,
author_sort Rizafizah Othaman,
title Essential oil with mosquito-repellent potential from plectranthus amboinicus leaves against aedes aegypti via human volunteer study
title_short Essential oil with mosquito-repellent potential from plectranthus amboinicus leaves against aedes aegypti via human volunteer study
title_full Essential oil with mosquito-repellent potential from plectranthus amboinicus leaves against aedes aegypti via human volunteer study
title_fullStr Essential oil with mosquito-repellent potential from plectranthus amboinicus leaves against aedes aegypti via human volunteer study
title_full_unstemmed Essential oil with mosquito-repellent potential from plectranthus amboinicus leaves against aedes aegypti via human volunteer study
title_sort essential oil with mosquito-repellent potential from plectranthus amboinicus leaves against aedes aegypti via human volunteer study
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2024
url http://journalarticle.ukm.my/24483/1/SS%201.pdf
http://journalarticle.ukm.my/24483/
https://www.ukm.my/jsm/english_journals/vol53num9_2024/contentsVol53num9_2024.html
_version_ 1816131604931674112
score 13.222552

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