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Salivary protein quantification among periodontitis patients with different disease severity: a pilot study

The salivary protein quantification reflecting the periodontal pathophysiology among patients with different periodontitis severity has not been well explored. This quantification is fundamental as the changes in the quantity of proteins in different severities of periodontitis can reflect the perio...

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Main Authors: Neelambigai R,, Poonisha Nsk,, Nurrul Shaqinah N,, Shahida Ms,, Nik Madihah Na,, Farinawati Y,
Format: Article
Language:English
Published: Penerbit UKM 2023
Online Access:http://journalarticle.ukm.my/24392/1/MH_4pdf_.pdf
http://journalarticle.ukm.my/24392/
https://medicineandhealthukm.com/article/salivary-protein-quantification-among-periodontitis-patients-different-disease-severity-pilo
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spelling my-ukm.journal.243922024-10-15T06:55:49Z http://journalarticle.ukm.my/24392/ Salivary protein quantification among periodontitis patients with different disease severity: a pilot study Neelambigai R, Poonisha Nsk, Nurrul Shaqinah N, Shahida Ms, Nik Madihah Na, Farinawati Y, The salivary protein quantification reflecting the periodontal pathophysiology among patients with different periodontitis severity has not been well explored. This quantification is fundamental as the changes in the quantity of proteins in different severities of periodontitis can reflect the periodontal complex alterations. This pilot study aimed to quantify and compare the salivary proteins by collecting unstimulated saliva from periodontitis patients and healthy individuals. The radioimmunoprecipitation assay (RIPA) protocol was used to extract protein from patients’ saliva and quantified using the Nanodrop Spectrophotometer 2000/2000c (A280) set at 280 nm wavelength. The mean concentration of salivary proteins was shown to be 26.48 + 2.95 mg/mL in healthy patients, 22.00 + 0.38 mg/mL in Stage 1 periodontitis patients, 27.34 + 1.61 mg/mL in Stage 2 periodontitis patients, 27.11 + 0.66 mg/mL in Stage 3 periodontitis patients and 24.89 + 1.91 mg/mL in Stage 4 periodontitis patients. However, the mean difference between all groups was not statistically significant. Within the limitations of this study, there was no difference in the salivary protein quantity among patients with different stages of periodontitis. Penerbit UKM 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24392/1/MH_4pdf_.pdf Neelambigai R, and Poonisha Nsk, and Nurrul Shaqinah N, and Shahida Ms, and Nik Madihah Na, and Farinawati Y, (2023) Salivary protein quantification among periodontitis patients with different disease severity: a pilot study. Medicine & Health, 18 (2). pp. 373-385. ISSN 1823-2140 https://medicineandhealthukm.com/article/salivary-protein-quantification-among-periodontitis-patients-different-disease-severity-pilo
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description The salivary protein quantification reflecting the periodontal pathophysiology among patients with different periodontitis severity has not been well explored. This quantification is fundamental as the changes in the quantity of proteins in different severities of periodontitis can reflect the periodontal complex alterations. This pilot study aimed to quantify and compare the salivary proteins by collecting unstimulated saliva from periodontitis patients and healthy individuals. The radioimmunoprecipitation assay (RIPA) protocol was used to extract protein from patients’ saliva and quantified using the Nanodrop Spectrophotometer 2000/2000c (A280) set at 280 nm wavelength. The mean concentration of salivary proteins was shown to be 26.48 + 2.95 mg/mL in healthy patients, 22.00 + 0.38 mg/mL in Stage 1 periodontitis patients, 27.34 + 1.61 mg/mL in Stage 2 periodontitis patients, 27.11 + 0.66 mg/mL in Stage 3 periodontitis patients and 24.89 + 1.91 mg/mL in Stage 4 periodontitis patients. However, the mean difference between all groups was not statistically significant. Within the limitations of this study, there was no difference in the salivary protein quantity among patients with different stages of periodontitis.
format Article
author Neelambigai R,
Poonisha Nsk,
Nurrul Shaqinah N,
Shahida Ms,
Nik Madihah Na,
Farinawati Y,
spellingShingle Neelambigai R,
Poonisha Nsk,
Nurrul Shaqinah N,
Shahida Ms,
Nik Madihah Na,
Farinawati Y,
Salivary protein quantification among periodontitis patients with different disease severity: a pilot study
author_facet Neelambigai R,
Poonisha Nsk,
Nurrul Shaqinah N,
Shahida Ms,
Nik Madihah Na,
Farinawati Y,
author_sort Neelambigai R,
title Salivary protein quantification among periodontitis patients with different disease severity: a pilot study
title_short Salivary protein quantification among periodontitis patients with different disease severity: a pilot study
title_full Salivary protein quantification among periodontitis patients with different disease severity: a pilot study
title_fullStr Salivary protein quantification among periodontitis patients with different disease severity: a pilot study
title_full_unstemmed Salivary protein quantification among periodontitis patients with different disease severity: a pilot study
title_sort salivary protein quantification among periodontitis patients with different disease severity: a pilot study
publisher Penerbit UKM
publishDate 2023
url http://journalarticle.ukm.my/24392/1/MH_4pdf_.pdf
http://journalarticle.ukm.my/24392/
https://medicineandhealthukm.com/article/salivary-protein-quantification-among-periodontitis-patients-different-disease-severity-pilo
_version_ 1814049138188222464
score 13.211869

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