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Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers

Banana (Musa spp.) is an economically important crop widely consumed all over the world. Understanding the genetic diversity and relatedness between various banana cultivars is important to continuously improve its fruit characteristics, nutritional value, and disease resistance. This study aimed to...

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Main Authors: Sharmilah Vetaryan,, Hooi, Wei Yeng, Lee, Yang Ping
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2024
Online Access:http://journalarticle.ukm.my/24242/1/ST%2010.pdf
http://journalarticle.ukm.my/24242/
https://www.ukm.my/jsm/english_journals/vol53num7_2024/contentsVol53num7_2024.html
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spelling my-ukm.journal.242422024-09-24T07:59:23Z http://journalarticle.ukm.my/24242/ Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers Sharmilah Vetaryan, Hooi, Wei Yeng Lee, Yang Ping Banana (Musa spp.) is an economically important crop widely consumed all over the world. Understanding the genetic diversity and relatedness between various banana cultivars is important to continuously improve its fruit characteristics, nutritional value, and disease resistance. This study aimed to develop a DNA fingerprinting panel using polymorphic simple sequence repeat (SSR) markers for the genetic characterisation of 11 banana cultivars in Malaysia. A total of 10 polymorphic SSR markers were developed into a multiplex PCR reaction and capillary electrophoresis to uniquely profile our collection of banana cultivars. The developed fingerprinting panel successfully amplified a total of 95 alleles, with 6 to 13 alleles per SSR marker. The average SSR marker polymorphic information content (PIC) value is 0.812, indicating the informativeness of the panel. Analysis of molecular variance (AMOVA) shows that 97% of the molecular variance from our banana collection is due to inter-variety genetic diversity, while the remaining 3% is due to intra-variety genetic diversity. A population structure analysis groups our collection of banana varieties according to the presence of at least one M. balbisiana (B) genome in their genetic makeup. The Cavendish variety, however, showed a distinct structure compared to the other cultivars. This SSR fingerprinting panel provides valuable insights into the genetic diversity and relatedness between banana cultivars in Malaysia. It has the potential to assist future banana breeding initiatives and serve as an effective quality control measure for verifying varieties in a tissue culture facility involved in banana planting materials production. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24242/1/ST%2010.pdf Sharmilah Vetaryan, and Hooi, Wei Yeng and Lee, Yang Ping (2024) Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers. Sains Malaysiana, 53 (7). pp. 1605-1616. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num7_2024/contentsVol53num7_2024.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Banana (Musa spp.) is an economically important crop widely consumed all over the world. Understanding the genetic diversity and relatedness between various banana cultivars is important to continuously improve its fruit characteristics, nutritional value, and disease resistance. This study aimed to develop a DNA fingerprinting panel using polymorphic simple sequence repeat (SSR) markers for the genetic characterisation of 11 banana cultivars in Malaysia. A total of 10 polymorphic SSR markers were developed into a multiplex PCR reaction and capillary electrophoresis to uniquely profile our collection of banana cultivars. The developed fingerprinting panel successfully amplified a total of 95 alleles, with 6 to 13 alleles per SSR marker. The average SSR marker polymorphic information content (PIC) value is 0.812, indicating the informativeness of the panel. Analysis of molecular variance (AMOVA) shows that 97% of the molecular variance from our banana collection is due to inter-variety genetic diversity, while the remaining 3% is due to intra-variety genetic diversity. A population structure analysis groups our collection of banana varieties according to the presence of at least one M. balbisiana (B) genome in their genetic makeup. The Cavendish variety, however, showed a distinct structure compared to the other cultivars. This SSR fingerprinting panel provides valuable insights into the genetic diversity and relatedness between banana cultivars in Malaysia. It has the potential to assist future banana breeding initiatives and serve as an effective quality control measure for verifying varieties in a tissue culture facility involved in banana planting materials production.
format Article
author Sharmilah Vetaryan,
Hooi, Wei Yeng
Lee, Yang Ping
spellingShingle Sharmilah Vetaryan,
Hooi, Wei Yeng
Lee, Yang Ping
Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers
author_facet Sharmilah Vetaryan,
Hooi, Wei Yeng
Lee, Yang Ping
author_sort Sharmilah Vetaryan,
title Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers
title_short Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers
title_full Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers
title_fullStr Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers
title_full_unstemmed Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers
title_sort assessment of genetic diversity and relatedness of local banana (musa spp.) cultivars in malaysia using simple sequence repeat (ssr) markers
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2024
url http://journalarticle.ukm.my/24242/1/ST%2010.pdf
http://journalarticle.ukm.my/24242/
https://www.ukm.my/jsm/english_journals/vol53num7_2024/contentsVol53num7_2024.html
_version_ 1811683423862915072
score 13.211869

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