Cover Image

Dimethyloxalylglycine (DMOG)-induced hypoxia promotes migratory and invasive properties of HCT116 colon cancer cell line

Hypoxia, a condition characterised by low oxygen levels, leads to increased production of a protein called hypoxiainducible factor-1 alpha (HIF-1α) in cancer cells. This protein is involved in driving processes such as vascularization, cytoskeletal reorganisation, and epithelial-to-mesenchymal trans...

Full description

Saved in:
Bibliographic Details
Main Authors: Nor Ezleen Qistina Ahmad,, Amirah Alhusna Mohd Yusoff,, Nur Fariesha Md Hashim,, Nurul Akmaryanti Abdullah,, Noraina Muhamad Zakuan,
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2024
Online Access:http://journalarticle.ukm.my/24123/1/SME%209.pdf
http://journalarticle.ukm.my/24123/
https://www.ukm.my/jsm/english_journals/vol53num6_2024/contentsVol53num6_2024.html
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-ukm.journal.24123
record_format eprints
spelling my-ukm.journal.241232024-09-05T04:36:57Z http://journalarticle.ukm.my/24123/ Dimethyloxalylglycine (DMOG)-induced hypoxia promotes migratory and invasive properties of HCT116 colon cancer cell line Nor Ezleen Qistina Ahmad, Amirah Alhusna Mohd Yusoff, Nur Fariesha Md Hashim, Nurul Akmaryanti Abdullah, Noraina Muhamad Zakuan, Hypoxia, a condition characterised by low oxygen levels, leads to increased production of a protein called hypoxiainducible factor-1 alpha (HIF-1α) in cancer cells. This protein is involved in driving processes such as vascularization, cytoskeletal reorganisation, and epithelial-to-mesenchymal transformation (EMT), which contribute to metastasis. Previous studies used hypoxic workstations, chambers, and incubators to evaluate the effects of hypoxia on colon cancer cell lines. In a cell culture model, hypoxic conditions can also be induced using dimethyloxalylglycine (DMOG) as the hypoxia-mimicking agent. This study aims to investigate the effects of DMOG-induced hypoxia on colon cancer metastasis, focusing on cell migration and invasion. HCT116 cells were subjected to hypoxic conditions by treating them with DMOG, and the expression of HIF-1α proteins was measured at various time points, followed by wound healing and invasion assays. It was found that HIF-1α protein expression increases after 6 h of DMOG induction and persists for 24 h. At 6 and 24 h, a significantly higher percentage of hypoxic cells migrated compared to normoxic cells. The invasion assay demonstrated that hypoxic cells were more invasive than normoxic cells within 24 h. Thus, the increase in migration and invasion of cells is comparable to the increase in HIF-1α expression at 6 and 24 h. These findings suggest that DMOG induces HIF-1α expression in colon cancer cells, leading to enhanced cell migration and invasiveness. The established model can be further utilised in gene knockdown or drug treatment studies to evaluate the effects of hypoxia on cancer cells. Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24123/1/SME%209.pdf Nor Ezleen Qistina Ahmad, and Amirah Alhusna Mohd Yusoff, and Nur Fariesha Md Hashim, and Nurul Akmaryanti Abdullah, and Noraina Muhamad Zakuan, (2024) Dimethyloxalylglycine (DMOG)-induced hypoxia promotes migratory and invasive properties of HCT116 colon cancer cell line. Sains Malaysiana, 53 (6). pp. 1333-1341. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num6_2024/contentsVol53num6_2024.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Hypoxia, a condition characterised by low oxygen levels, leads to increased production of a protein called hypoxiainducible factor-1 alpha (HIF-1α) in cancer cells. This protein is involved in driving processes such as vascularization, cytoskeletal reorganisation, and epithelial-to-mesenchymal transformation (EMT), which contribute to metastasis. Previous studies used hypoxic workstations, chambers, and incubators to evaluate the effects of hypoxia on colon cancer cell lines. In a cell culture model, hypoxic conditions can also be induced using dimethyloxalylglycine (DMOG) as the hypoxia-mimicking agent. This study aims to investigate the effects of DMOG-induced hypoxia on colon cancer metastasis, focusing on cell migration and invasion. HCT116 cells were subjected to hypoxic conditions by treating them with DMOG, and the expression of HIF-1α proteins was measured at various time points, followed by wound healing and invasion assays. It was found that HIF-1α protein expression increases after 6 h of DMOG induction and persists for 24 h. At 6 and 24 h, a significantly higher percentage of hypoxic cells migrated compared to normoxic cells. The invasion assay demonstrated that hypoxic cells were more invasive than normoxic cells within 24 h. Thus, the increase in migration and invasion of cells is comparable to the increase in HIF-1α expression at 6 and 24 h. These findings suggest that DMOG induces HIF-1α expression in colon cancer cells, leading to enhanced cell migration and invasiveness. The established model can be further utilised in gene knockdown or drug treatment studies to evaluate the effects of hypoxia on cancer cells.
format Article
author Nor Ezleen Qistina Ahmad,
Amirah Alhusna Mohd Yusoff,
Nur Fariesha Md Hashim,
Nurul Akmaryanti Abdullah,
Noraina Muhamad Zakuan,
spellingShingle Nor Ezleen Qistina Ahmad,
Amirah Alhusna Mohd Yusoff,
Nur Fariesha Md Hashim,
Nurul Akmaryanti Abdullah,
Noraina Muhamad Zakuan,
Dimethyloxalylglycine (DMOG)-induced hypoxia promotes migratory and invasive properties of HCT116 colon cancer cell line
author_facet Nor Ezleen Qistina Ahmad,
Amirah Alhusna Mohd Yusoff,
Nur Fariesha Md Hashim,
Nurul Akmaryanti Abdullah,
Noraina Muhamad Zakuan,
author_sort Nor Ezleen Qistina Ahmad,
title Dimethyloxalylglycine (DMOG)-induced hypoxia promotes migratory and invasive properties of HCT116 colon cancer cell line
title_short Dimethyloxalylglycine (DMOG)-induced hypoxia promotes migratory and invasive properties of HCT116 colon cancer cell line
title_full Dimethyloxalylglycine (DMOG)-induced hypoxia promotes migratory and invasive properties of HCT116 colon cancer cell line
title_fullStr Dimethyloxalylglycine (DMOG)-induced hypoxia promotes migratory and invasive properties of HCT116 colon cancer cell line
title_full_unstemmed Dimethyloxalylglycine (DMOG)-induced hypoxia promotes migratory and invasive properties of HCT116 colon cancer cell line
title_sort dimethyloxalylglycine (dmog)-induced hypoxia promotes migratory and invasive properties of hct116 colon cancer cell line
publisher Universiti Kebangsaan Malaysia
publishDate 2024
url http://journalarticle.ukm.my/24123/1/SME%209.pdf
http://journalarticle.ukm.my/24123/
https://www.ukm.my/jsm/english_journals/vol53num6_2024/contentsVol53num6_2024.html
_version_ 1811683408318824448
score 13.211869

Similar Items