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Antioxidant and alpha-glucosidase inhibitory activity of durio zibethinus l. clone 175 (Durian Udang Merah) shell and UHPLC-Orbitrap-MS profiling of the extract

Durio zibethinus or ‘durian’ is a well-known seasonal fruit of Southeast Asia and has been called the ‘King of Fruit’. The popularity of durian has led to large production of this crop in Malaysia, consequently creating a huge agricultural waste including the shell. Despite illness remedies from var...

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Main Authors: Wan Muhd Hatim Wan Zain,, Ahmed Mediani,, Nur Khaleeda Zulaikha Zolkeflee,, Pei, Lou Wong, Faridah Abas,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2024
Online Access:http://journalarticle.ukm.my/24119/1/SME%205.pdf
http://journalarticle.ukm.my/24119/
https://www.ukm.my/jsm/english_journals/vol53num6_2024/contentsVol53num6_2024.html
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spelling my-ukm.journal.241192024-09-05T03:48:52Z http://journalarticle.ukm.my/24119/ Antioxidant and alpha-glucosidase inhibitory activity of durio zibethinus l. clone 175 (Durian Udang Merah) shell and UHPLC-Orbitrap-MS profiling of the extract Wan Muhd Hatim Wan Zain, Ahmed Mediani, Nur Khaleeda Zulaikha Zolkeflee, Pei, Lou Wong Faridah Abas, Durio zibethinus or ‘durian’ is a well-known seasonal fruit of Southeast Asia and has been called the ‘King of Fruit’. The popularity of durian has led to large production of this crop in Malaysia, consequently creating a huge agricultural waste including the shell. Despite illness remedies from various parts of durian, the information on phytochemical constituents and bioactivities of durian shells remained scarce. Therefore, this study aimed to evaluate the total phenolic content (TPC), antioxidant, and α-glucosidase inhibitory activities of D. zibethinus shell extracted with different ethanol percentage (0%, 50%, and 100%). Results showed that the 100% ethanolic extract exhibited the highest 2,2-dipheny-1-picrylhydrazyl (DPPH) free radical scavenging activity with an IC50 value of 96.91 ± 1.09 µg/ mL. Furthermore, the 50% ethanol extract exhibited the highest TPC with 130.57 ± 1.92 mg GAE/g crude extract, and nitric oxide (NO) scavenging activity with an IC50 value of 435.30 ± 3.41 µg/mL. Both 50% and 100% ethanolic extracts of D. zibethinus shell exhibited great potential in α-glucosidase inhibitory activity with IC50 values of 1.99 ± 0.90 and 4.53 ± 0.21 µg/mL, respectively. Thus, the bioactive compounds in 100% ethanolic extract of D. zibethinus were profiled by ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis. A total of 20 compounds were tentatively identified, including flavonoids, alkaloids, benzofurans, terpenoids, pentose phosphate, organosulfur compounds, organooxygen compounds, polyketides, carotene, carboxylic acid, coumarin, and stigmasterol. In conclusion, durian shell exhibits potential for future applications driven by its inherent pharmacological benefits, consequently contributing to waste reduction. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24119/1/SME%205.pdf Wan Muhd Hatim Wan Zain, and Ahmed Mediani, and Nur Khaleeda Zulaikha Zolkeflee, and Pei, Lou Wong and Faridah Abas, (2024) Antioxidant and alpha-glucosidase inhibitory activity of durio zibethinus l. clone 175 (Durian Udang Merah) shell and UHPLC-Orbitrap-MS profiling of the extract. Sains Malaysiana, 53 (6). pp. 1281-1293. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num6_2024/contentsVol53num6_2024.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Durio zibethinus or ‘durian’ is a well-known seasonal fruit of Southeast Asia and has been called the ‘King of Fruit’. The popularity of durian has led to large production of this crop in Malaysia, consequently creating a huge agricultural waste including the shell. Despite illness remedies from various parts of durian, the information on phytochemical constituents and bioactivities of durian shells remained scarce. Therefore, this study aimed to evaluate the total phenolic content (TPC), antioxidant, and α-glucosidase inhibitory activities of D. zibethinus shell extracted with different ethanol percentage (0%, 50%, and 100%). Results showed that the 100% ethanolic extract exhibited the highest 2,2-dipheny-1-picrylhydrazyl (DPPH) free radical scavenging activity with an IC50 value of 96.91 ± 1.09 µg/ mL. Furthermore, the 50% ethanol extract exhibited the highest TPC with 130.57 ± 1.92 mg GAE/g crude extract, and nitric oxide (NO) scavenging activity with an IC50 value of 435.30 ± 3.41 µg/mL. Both 50% and 100% ethanolic extracts of D. zibethinus shell exhibited great potential in α-glucosidase inhibitory activity with IC50 values of 1.99 ± 0.90 and 4.53 ± 0.21 µg/mL, respectively. Thus, the bioactive compounds in 100% ethanolic extract of D. zibethinus were profiled by ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis. A total of 20 compounds were tentatively identified, including flavonoids, alkaloids, benzofurans, terpenoids, pentose phosphate, organosulfur compounds, organooxygen compounds, polyketides, carotene, carboxylic acid, coumarin, and stigmasterol. In conclusion, durian shell exhibits potential for future applications driven by its inherent pharmacological benefits, consequently contributing to waste reduction.
format Article
author Wan Muhd Hatim Wan Zain,
Ahmed Mediani,
Nur Khaleeda Zulaikha Zolkeflee,
Pei, Lou Wong
Faridah Abas,
spellingShingle Wan Muhd Hatim Wan Zain,
Ahmed Mediani,
Nur Khaleeda Zulaikha Zolkeflee,
Pei, Lou Wong
Faridah Abas,
Antioxidant and alpha-glucosidase inhibitory activity of durio zibethinus l. clone 175 (Durian Udang Merah) shell and UHPLC-Orbitrap-MS profiling of the extract
author_facet Wan Muhd Hatim Wan Zain,
Ahmed Mediani,
Nur Khaleeda Zulaikha Zolkeflee,
Pei, Lou Wong
Faridah Abas,
author_sort Wan Muhd Hatim Wan Zain,
title Antioxidant and alpha-glucosidase inhibitory activity of durio zibethinus l. clone 175 (Durian Udang Merah) shell and UHPLC-Orbitrap-MS profiling of the extract
title_short Antioxidant and alpha-glucosidase inhibitory activity of durio zibethinus l. clone 175 (Durian Udang Merah) shell and UHPLC-Orbitrap-MS profiling of the extract
title_full Antioxidant and alpha-glucosidase inhibitory activity of durio zibethinus l. clone 175 (Durian Udang Merah) shell and UHPLC-Orbitrap-MS profiling of the extract
title_fullStr Antioxidant and alpha-glucosidase inhibitory activity of durio zibethinus l. clone 175 (Durian Udang Merah) shell and UHPLC-Orbitrap-MS profiling of the extract
title_full_unstemmed Antioxidant and alpha-glucosidase inhibitory activity of durio zibethinus l. clone 175 (Durian Udang Merah) shell and UHPLC-Orbitrap-MS profiling of the extract
title_sort antioxidant and alpha-glucosidase inhibitory activity of durio zibethinus l. clone 175 (durian udang merah) shell and uhplc-orbitrap-ms profiling of the extract
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2024
url http://journalarticle.ukm.my/24119/1/SME%205.pdf
http://journalarticle.ukm.my/24119/
https://www.ukm.my/jsm/english_journals/vol53num6_2024/contentsVol53num6_2024.html
_version_ 1811683407733719040
score 13.211869

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