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Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS

The plant of Metroxylon sago Rottb. which is found abundantly in Indonesia, Malaysia, Philippines, and Papua New Guinea, is famous as carbohydrate producing plants. The sago fruit has not been yet extensively used, although they are rich in oligosaccharides which is potential as prebiotic. Sago frui...

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Main Authors: Fadhilla, Reza, Yuliana, Nancy Dewi, Kusnandar, Feri, Kusumaningrum, Harsi Dewantari
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2024
Online Access:http://journalarticle.ukm.my/24118/1/SME%204.pdf
http://journalarticle.ukm.my/24118/
https://www.ukm.my/jsm/english_journals/vol53num6_2024/contentsVol53num6_2024.html
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spelling my-ukm.journal.241182024-09-05T03:41:11Z http://journalarticle.ukm.my/24118/ Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS Fadhilla, Reza Yuliana, Nancy Dewi Kusnandar, Feri Kusumaningrum, Harsi Dewantari The plant of Metroxylon sago Rottb. which is found abundantly in Indonesia, Malaysia, Philippines, and Papua New Guinea, is famous as carbohydrate producing plants. The sago fruit has not been yet extensively used, although they are rich in oligosaccharides which is potential as prebiotic. Sago fruit flour was studied as growth substrate for five selected probiotics and phytochemical profile of its methanol extracts were determined. All bacteria grew well up to 24 h on the growth medium containing sago fruit flour as carbohydrate substitute. However, Lacticaseibacillus rhamnosus FNCC 0099 and Lactobacillus acidophilus FNCC 0051 showed viability stability up to 72 h, while the others were slightly decreased. The sago fruit flour contained 20.4 mg/g total sugars, 14.8 mg QE/g flavonoids, and 36.7 mg GAE/g phenolics. After extraction of the fruit flour with 80% methanol using an ultrasonicator at 55 °C at 40 kHz for 40 min, the extract was analysed for its phytochemical profiles using untargeted LC-MS/MS screening with negative ionization mode. Seven compounds categorised in three distinct groups i.e., sugar alcohol, plant glycosides and fatty acids, have been identified as having possible prebiotic activity. These include ((1xi)-1,5-anhydro-2,3,6-tris-O-(carboxymethyl)-1-methyl-4-Omethyl-D-glucitol), quercitrin, (15Z)-9,12,13-trihydroxy-15-octadecenoic acid, corchorifatty acid F, 3,5-dihydroxy-2- (4-hydroxyphenyl)-4-oxo-3,4-dihydro-2H-chromen-7-yl-hexopyranoside, gynocardin, and (4-methylumbelliferone)-βD-glucopyranoside. As the sago fruit flour has been proved as potential prebiotic in this study, its extensive prebiotics activity, prebiotic index, and stability under digestive condition will be subjected for further study. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24118/1/SME%204.pdf Fadhilla, Reza and Yuliana, Nancy Dewi and Kusnandar, Feri and Kusumaningrum, Harsi Dewantari (2024) Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS. Sains Malaysiana, 53 (6). pp. 1269-1280. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num6_2024/contentsVol53num6_2024.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description The plant of Metroxylon sago Rottb. which is found abundantly in Indonesia, Malaysia, Philippines, and Papua New Guinea, is famous as carbohydrate producing plants. The sago fruit has not been yet extensively used, although they are rich in oligosaccharides which is potential as prebiotic. Sago fruit flour was studied as growth substrate for five selected probiotics and phytochemical profile of its methanol extracts were determined. All bacteria grew well up to 24 h on the growth medium containing sago fruit flour as carbohydrate substitute. However, Lacticaseibacillus rhamnosus FNCC 0099 and Lactobacillus acidophilus FNCC 0051 showed viability stability up to 72 h, while the others were slightly decreased. The sago fruit flour contained 20.4 mg/g total sugars, 14.8 mg QE/g flavonoids, and 36.7 mg GAE/g phenolics. After extraction of the fruit flour with 80% methanol using an ultrasonicator at 55 °C at 40 kHz for 40 min, the extract was analysed for its phytochemical profiles using untargeted LC-MS/MS screening with negative ionization mode. Seven compounds categorised in three distinct groups i.e., sugar alcohol, plant glycosides and fatty acids, have been identified as having possible prebiotic activity. These include ((1xi)-1,5-anhydro-2,3,6-tris-O-(carboxymethyl)-1-methyl-4-Omethyl-D-glucitol), quercitrin, (15Z)-9,12,13-trihydroxy-15-octadecenoic acid, corchorifatty acid F, 3,5-dihydroxy-2- (4-hydroxyphenyl)-4-oxo-3,4-dihydro-2H-chromen-7-yl-hexopyranoside, gynocardin, and (4-methylumbelliferone)-βD-glucopyranoside. As the sago fruit flour has been proved as potential prebiotic in this study, its extensive prebiotics activity, prebiotic index, and stability under digestive condition will be subjected for further study.
format Article
author Fadhilla, Reza
Yuliana, Nancy Dewi
Kusnandar, Feri
Kusumaningrum, Harsi Dewantari
spellingShingle Fadhilla, Reza
Yuliana, Nancy Dewi
Kusnandar, Feri
Kusumaningrum, Harsi Dewantari
Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS
author_facet Fadhilla, Reza
Yuliana, Nancy Dewi
Kusnandar, Feri
Kusumaningrum, Harsi Dewantari
author_sort Fadhilla, Reza
title Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS
title_short Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS
title_full Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS
title_fullStr Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS
title_full_unstemmed Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS
title_sort metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by lc-ms/ms
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2024
url http://journalarticle.ukm.my/24118/1/SME%204.pdf
http://journalarticle.ukm.my/24118/
https://www.ukm.my/jsm/english_journals/vol53num6_2024/contentsVol53num6_2024.html
_version_ 1811683407604744192
score 13.211869

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