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Development of real-time PCR method for detection of Xanthomonas campestris pv. juglandis causing walnut bacterial blight

Walnut is one of the important economic tree species in the world. For the past few years, with the continuous expansion of walnut planting area, walnut bacterial blight caused by Xanthomonas campestris pv. juglandis has critically affected walnut production and caused economic losses. In this resea...

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Main Authors: Dong, Yuqing, Xie, Ling, Zhu, Hanmingyue, Zhu, Tianhui
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2024
Online Access:http://journalarticle.ukm.my/24031/1/SEE%205.pdf
http://journalarticle.ukm.my/24031/
https://www.ukm.my/jsm/english_journals/vol53num5_2024/contentsVol53num5_2024.html
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spelling my-ukm.journal.240312024-08-19T02:31:46Z http://journalarticle.ukm.my/24031/ Development of real-time PCR method for detection of Xanthomonas campestris pv. juglandis causing walnut bacterial blight Dong, Yuqing Xie, Ling Zhu, Hanmingyue Zhu, Tianhui Walnut is one of the important economic tree species in the world. For the past few years, with the continuous expansion of walnut planting area, walnut bacterial blight caused by Xanthomonas campestris pv. juglandis has critically affected walnut production and caused economic losses. In this research, by comparing the gene sequences of X. campestris pv. juglandis and its closely related bacterial species, the 16S rDNA gene sequence was selected, and the specific primers were evaluated using PCR. Subsequently, the real-time fluorescence PCR specific detection method of X. campestris pv. juglandis was established and optimized. The specificity of real-time PCR was verified for 23 strains isolated from walnut and its surrounding soil, including eight target pathogens. The specific amplification was possible even when the cDNA template concentration was 7.0×10-5 µg/mL, hence, the detection sensitivity of the standard method is 7.0×10-5 µg/mL. The reliability of the established real-time PCR detection method in field detection was verified by the identification of target bacteria in 8 randomly collected healthy leaves and leaves of suspected walnut blight. This method laid a foundation for the early diagnosis of X. campestris pv. juglandis. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24031/1/SEE%205.pdf Dong, Yuqing and Xie, Ling and Zhu, Hanmingyue and Zhu, Tianhui (2024) Development of real-time PCR method for detection of Xanthomonas campestris pv. juglandis causing walnut bacterial blight. Sains Malaysiana, 53 (5). pp. 1033-1042. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num5_2024/contentsVol53num5_2024.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Walnut is one of the important economic tree species in the world. For the past few years, with the continuous expansion of walnut planting area, walnut bacterial blight caused by Xanthomonas campestris pv. juglandis has critically affected walnut production and caused economic losses. In this research, by comparing the gene sequences of X. campestris pv. juglandis and its closely related bacterial species, the 16S rDNA gene sequence was selected, and the specific primers were evaluated using PCR. Subsequently, the real-time fluorescence PCR specific detection method of X. campestris pv. juglandis was established and optimized. The specificity of real-time PCR was verified for 23 strains isolated from walnut and its surrounding soil, including eight target pathogens. The specific amplification was possible even when the cDNA template concentration was 7.0×10-5 µg/mL, hence, the detection sensitivity of the standard method is 7.0×10-5 µg/mL. The reliability of the established real-time PCR detection method in field detection was verified by the identification of target bacteria in 8 randomly collected healthy leaves and leaves of suspected walnut blight. This method laid a foundation for the early diagnosis of X. campestris pv. juglandis.
format Article
author Dong, Yuqing
Xie, Ling
Zhu, Hanmingyue
Zhu, Tianhui
spellingShingle Dong, Yuqing
Xie, Ling
Zhu, Hanmingyue
Zhu, Tianhui
Development of real-time PCR method for detection of Xanthomonas campestris pv. juglandis causing walnut bacterial blight
author_facet Dong, Yuqing
Xie, Ling
Zhu, Hanmingyue
Zhu, Tianhui
author_sort Dong, Yuqing
title Development of real-time PCR method for detection of Xanthomonas campestris pv. juglandis causing walnut bacterial blight
title_short Development of real-time PCR method for detection of Xanthomonas campestris pv. juglandis causing walnut bacterial blight
title_full Development of real-time PCR method for detection of Xanthomonas campestris pv. juglandis causing walnut bacterial blight
title_fullStr Development of real-time PCR method for detection of Xanthomonas campestris pv. juglandis causing walnut bacterial blight
title_full_unstemmed Development of real-time PCR method for detection of Xanthomonas campestris pv. juglandis causing walnut bacterial blight
title_sort development of real-time pcr method for detection of xanthomonas campestris pv. juglandis causing walnut bacterial blight
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2024
url http://journalarticle.ukm.my/24031/1/SEE%205.pdf
http://journalarticle.ukm.my/24031/
https://www.ukm.my/jsm/english_journals/vol53num5_2024/contentsVol53num5_2024.html
_version_ 1809139047873904640
score 13.211869

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