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An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds

The aim of this study was to establish an effective protocol for callus induction from the seed explants of Solanum dubium and to investigate the callus extract ability in milk clotting activity. The effects of growth regulator, basal media strength and sucrose were studied using different concentra...

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Main Authors: Fatima Musbah Abbas,, ElBushra ElSheikh ElNur,, Normah Mohd Noor Eisa Algaali,, Zainon Mohd Ali,, Roohaida Othman,
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2011
Online Access:http://journalarticle.ukm.my/2394/1/09_Fatima.pdf
http://journalarticle.ukm.my/2394/
http://www.ukm.my/jsm/
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spelling my-ukm.journal.23942016-12-14T06:31:29Z http://journalarticle.ukm.my/2394/ An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds Fatima Musbah Abbas, ElBushra ElSheikh ElNur, Normah Mohd Noor Eisa Algaali, Zainon Mohd Ali, Roohaida Othman, The aim of this study was to establish an effective protocol for callus induction from the seed explants of Solanum dubium and to investigate the callus extract ability in milk clotting activity. The effects of growth regulator, basal media strength and sucrose were studied using different concentrations (0.5, 1.0, 1.5, and 2.0 mg/L) of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), naphthalene acetic acid (NAA) and 2,4-dichlorophenoxy-acetic acid (2,4-D) alone or in combination with 0.5 mg/L of 6-benzylamino purine (BAP). For milk clotting activity, about 50 or 100 μL extracts of seed callus was mixed with 2 mL 50% milk held at 55°C for 5 and 10 min until milk clotting occurred. The results showed that NAA alone or in combination with BAP gave a higher callusing percentage (80 to100%) compared to the other plant growth regulators at the same concentrations. When an auxin was supplied in combination with BAP, a significant increase in callusing percentage or degree of callusing was observed. The time required for callus to be developed was shortened and the quality of the induced callus improved. An increase in callus growth in low sucrose (10 g/L) concentration was found be comparable (88%) to high sucrose concentration (30 g/L; 60%). Crude extracts obtained from S. dubium callus were shown to exhibit milk clotting activity. Universiti Kebangsaan Malaysia 2011-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/2394/1/09_Fatima.pdf Fatima Musbah Abbas, and ElBushra ElSheikh ElNur, and Normah Mohd Noor Eisa Algaali, and Zainon Mohd Ali, and Roohaida Othman, (2011) An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds. Sains Malaysiana, 40 (4). pp. 339-343. ISSN 0126-6039 http://www.ukm.my/jsm/
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description The aim of this study was to establish an effective protocol for callus induction from the seed explants of Solanum dubium and to investigate the callus extract ability in milk clotting activity. The effects of growth regulator, basal media strength and sucrose were studied using different concentrations (0.5, 1.0, 1.5, and 2.0 mg/L) of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), naphthalene acetic acid (NAA) and 2,4-dichlorophenoxy-acetic acid (2,4-D) alone or in combination with 0.5 mg/L of 6-benzylamino purine (BAP). For milk clotting activity, about 50 or 100 μL extracts of seed callus was mixed with 2 mL 50% milk held at 55°C for 5 and 10 min until milk clotting occurred. The results showed that NAA alone or in combination with BAP gave a higher callusing percentage (80 to100%) compared to the other plant growth regulators at the same concentrations. When an auxin was supplied in combination with BAP, a significant increase in callusing percentage or degree of callusing was observed. The time required for callus to be developed was shortened and the quality of the induced callus improved. An increase in callus growth in low sucrose (10 g/L) concentration was found be comparable (88%) to high sucrose concentration (30 g/L; 60%). Crude extracts obtained from S. dubium callus were shown to exhibit milk clotting activity.
format Article
author Fatima Musbah Abbas,
ElBushra ElSheikh ElNur,
Normah Mohd Noor Eisa Algaali,
Zainon Mohd Ali,
Roohaida Othman,
spellingShingle Fatima Musbah Abbas,
ElBushra ElSheikh ElNur,
Normah Mohd Noor Eisa Algaali,
Zainon Mohd Ali,
Roohaida Othman,
An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds
author_facet Fatima Musbah Abbas,
ElBushra ElSheikh ElNur,
Normah Mohd Noor Eisa Algaali,
Zainon Mohd Ali,
Roohaida Othman,
author_sort Fatima Musbah Abbas,
title An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds
title_short An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds
title_full An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds
title_fullStr An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds
title_full_unstemmed An effective protocol for callus induction with milk clotting activity from solanum dubiumSeeds
title_sort effective protocol for callus induction with milk clotting activity from solanum dubiumseeds
publisher Universiti Kebangsaan Malaysia
publishDate 2011
url http://journalarticle.ukm.my/2394/1/09_Fatima.pdf
http://journalarticle.ukm.my/2394/
http://www.ukm.my/jsm/
_version_ 1643735365457018880
score 13.211869

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