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Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus

Gestational Diabetes Mellitus (GDM) is a common but temporary type of diabetes that develops among 10-20% of all pregnant women. It is a major cause of several pre- and post-pregnancy diseases in both mother and offspring. The main causative is altered pregnancy hormones that lead to deficient gluco...

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Main Authors: Kaleem, Afshan, Anwar, Tehreem, Iftikhar, Aqsa, Abdullah, Roheena, Mehwish Iqtedar, Mehwish, Aftab, Mahwish
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2024
Online Access:http://journalarticle.ukm.my/23753/1/SMT%2015.pdf
http://journalarticle.ukm.my/23753/
https://www.ukm.my/jsm/english_journals/vol53num3_2024/contentsVol53num3_2024.html
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spelling my-ukm.journal.237532024-07-04T04:10:10Z http://journalarticle.ukm.my/23753/ Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus Kaleem, Afshan Anwar, Tehreem Iftikhar, Aqsa Abdullah, Roheena Mehwish Iqtedar, Mehwish Aftab, Mahwish Gestational Diabetes Mellitus (GDM) is a common but temporary type of diabetes that develops among 10-20% of all pregnant women. It is a major cause of several pre- and post-pregnancy diseases in both mother and offspring. The main causative is altered pregnancy hormones that lead to deficient glucose metabolism. Complications can be preeclampsia, caesarean sections and cardiovascular diseases. This study was designed to determine the phosphorylated and glycosylated proteins present in GDM and to investigate the role of their post-translational modifications (PTMs) in the pathogenesis of GDM. The study was performed on 30 blood samples from pregnant diabetic women (diseased), 30 pregnant non-diabetic women (control) and 30 women without pregnancy and gestational diabetes (normal). Protein extraction was done from blood plasma, and phosphorylated and glycosylated proteins were determined by EnzymeLinked Immuno-Sorbent Assay (ELISA) by use of specific antibodies. This study showed that proteins found in GDM are highly phosphorylated and O-glycosylated (p<0.01). Furthermore, a bioinformatic investigation was done, which showed that blood coagulation proteins such as the Fibrinogen alpha chain and lipid metabolism-regulating proteins apolipoprotein E, L1 and C-III showed a high potential for phosphorylation, which suggests that phosphorylation can be used as a therapeutic marker in GDM. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/23753/1/SMT%2015.pdf Kaleem, Afshan and Anwar, Tehreem and Iftikhar, Aqsa and Abdullah, Roheena and Mehwish Iqtedar, Mehwish and Aftab, Mahwish (2024) Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus. Sains Malaysiana, 53 (3). pp. 681-689. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num3_2024/contentsVol53num3_2024.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Gestational Diabetes Mellitus (GDM) is a common but temporary type of diabetes that develops among 10-20% of all pregnant women. It is a major cause of several pre- and post-pregnancy diseases in both mother and offspring. The main causative is altered pregnancy hormones that lead to deficient glucose metabolism. Complications can be preeclampsia, caesarean sections and cardiovascular diseases. This study was designed to determine the phosphorylated and glycosylated proteins present in GDM and to investigate the role of their post-translational modifications (PTMs) in the pathogenesis of GDM. The study was performed on 30 blood samples from pregnant diabetic women (diseased), 30 pregnant non-diabetic women (control) and 30 women without pregnancy and gestational diabetes (normal). Protein extraction was done from blood plasma, and phosphorylated and glycosylated proteins were determined by EnzymeLinked Immuno-Sorbent Assay (ELISA) by use of specific antibodies. This study showed that proteins found in GDM are highly phosphorylated and O-glycosylated (p<0.01). Furthermore, a bioinformatic investigation was done, which showed that blood coagulation proteins such as the Fibrinogen alpha chain and lipid metabolism-regulating proteins apolipoprotein E, L1 and C-III showed a high potential for phosphorylation, which suggests that phosphorylation can be used as a therapeutic marker in GDM.
format Article
author Kaleem, Afshan
Anwar, Tehreem
Iftikhar, Aqsa
Abdullah, Roheena
Mehwish Iqtedar, Mehwish
Aftab, Mahwish
spellingShingle Kaleem, Afshan
Anwar, Tehreem
Iftikhar, Aqsa
Abdullah, Roheena
Mehwish Iqtedar, Mehwish
Aftab, Mahwish
Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus
author_facet Kaleem, Afshan
Anwar, Tehreem
Iftikhar, Aqsa
Abdullah, Roheena
Mehwish Iqtedar, Mehwish
Aftab, Mahwish
author_sort Kaleem, Afshan
title Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus
title_short Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus
title_full Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus
title_fullStr Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus
title_full_unstemmed Investigation of phosphorylated and O-glycosylated proteins in gestational diabetes mellitus
title_sort investigation of phosphorylated and o-glycosylated proteins in gestational diabetes mellitus
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2024
url http://journalarticle.ukm.my/23753/1/SMT%2015.pdf
http://journalarticle.ukm.my/23753/
https://www.ukm.my/jsm/english_journals/vol53num3_2024/contentsVol53num3_2024.html
_version_ 1804066006001254400
score 13.211869

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