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Functional analysis of cag pathogenicity island diversity in helicobacter pylori isolated from multi-ethnic patients in Malaysia

cagPAI is an important pathogenic marker contributing to disease severity caused by H. pylori infection, encoding proteins for type four secretion systems (T4SS) and the pro-inflammatory cytokines (IL-8) induction in infected host. The heterogeneity of cagPAI genes results in different clinical outc...

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Main Authors: Shaza Azlin Razak,, Alfizah Hanafiah,, Asif Sukri,, Neoh, Hui-Min,, Noraziah Mohamad Zin,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2024
Online Access:http://journalarticle.ukm.my/23745/1/SMT%2010.pdf
http://journalarticle.ukm.my/23745/
https://www.ukm.my/jsm/english_journals/vol53num3_2024/contentsVol53num3_2024.html
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spelling my-ukm.journal.237452024-07-02T05:17:15Z http://journalarticle.ukm.my/23745/ Functional analysis of cag pathogenicity island diversity in helicobacter pylori isolated from multi-ethnic patients in Malaysia Shaza Azlin Razak, Alfizah Hanafiah, Asif Sukri, Neoh, Hui-Min, Noraziah Mohamad Zin, cagPAI is an important pathogenic marker contributing to disease severity caused by H. pylori infection, encoding proteins for type four secretion systems (T4SS) and the pro-inflammatory cytokines (IL-8) induction in infected host. The heterogeneity of cagPAI genes results in different clinical outcomes in H. pylori infection. The aim of this study was to investigate the functional diversity of cagPAI genes in clinical H. pylori strains isolated from patients with gastroduodenal diseases. Among the isolates investigated, 6 out of 27 (22.2%) harbor intact cagPAI, 3 strains (11.1%) have complete absence of cagPAI, and 18 strains (66.7%) contain a partially deleted island. IL-8 concentration is observed significantly higher among cagPAI-positive isolates (P = 0.045). Of note, IL-8 secretion in the gastric cells infected with H. pylori strains isolated from Malay patients was significantly higher than the gastric cells infected with the strains from non-Malay patients (P = 0.046). The secretion of IL-8 appears in similarly high among intact and partially deleted cagPAI isolates. East Asian cagA isolates induce a notably higher level of IL-8 than that of Western cagA. Inflammatory activities are observed and IL-8 concentration in the milder stage was found in significantly higher quantities than in the severe stage (P = 0.027). The ‘hummingbird’ phenotype was observed to cause severe effects in infected cells with cagPAI-intact H. pylori. The genetic variability of cagPAI isolates from multi-ethnicity patients may have unique implications for disease outcomes with respect to proinflammatory secretion. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/23745/1/SMT%2010.pdf Shaza Azlin Razak, and Alfizah Hanafiah, and Asif Sukri, and Neoh, Hui-Min, and Noraziah Mohamad Zin, (2024) Functional analysis of cag pathogenicity island diversity in helicobacter pylori isolated from multi-ethnic patients in Malaysia. Sains Malaysiana, 53 (3). pp. 605-621. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num3_2024/contentsVol53num3_2024.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description cagPAI is an important pathogenic marker contributing to disease severity caused by H. pylori infection, encoding proteins for type four secretion systems (T4SS) and the pro-inflammatory cytokines (IL-8) induction in infected host. The heterogeneity of cagPAI genes results in different clinical outcomes in H. pylori infection. The aim of this study was to investigate the functional diversity of cagPAI genes in clinical H. pylori strains isolated from patients with gastroduodenal diseases. Among the isolates investigated, 6 out of 27 (22.2%) harbor intact cagPAI, 3 strains (11.1%) have complete absence of cagPAI, and 18 strains (66.7%) contain a partially deleted island. IL-8 concentration is observed significantly higher among cagPAI-positive isolates (P = 0.045). Of note, IL-8 secretion in the gastric cells infected with H. pylori strains isolated from Malay patients was significantly higher than the gastric cells infected with the strains from non-Malay patients (P = 0.046). The secretion of IL-8 appears in similarly high among intact and partially deleted cagPAI isolates. East Asian cagA isolates induce a notably higher level of IL-8 than that of Western cagA. Inflammatory activities are observed and IL-8 concentration in the milder stage was found in significantly higher quantities than in the severe stage (P = 0.027). The ‘hummingbird’ phenotype was observed to cause severe effects in infected cells with cagPAI-intact H. pylori. The genetic variability of cagPAI isolates from multi-ethnicity patients may have unique implications for disease outcomes with respect to proinflammatory secretion.
format Article
author Shaza Azlin Razak,
Alfizah Hanafiah,
Asif Sukri,
Neoh, Hui-Min,
Noraziah Mohamad Zin,
spellingShingle Shaza Azlin Razak,
Alfizah Hanafiah,
Asif Sukri,
Neoh, Hui-Min,
Noraziah Mohamad Zin,
Functional analysis of cag pathogenicity island diversity in helicobacter pylori isolated from multi-ethnic patients in Malaysia
author_facet Shaza Azlin Razak,
Alfizah Hanafiah,
Asif Sukri,
Neoh, Hui-Min,
Noraziah Mohamad Zin,
author_sort Shaza Azlin Razak,
title Functional analysis of cag pathogenicity island diversity in helicobacter pylori isolated from multi-ethnic patients in Malaysia
title_short Functional analysis of cag pathogenicity island diversity in helicobacter pylori isolated from multi-ethnic patients in Malaysia
title_full Functional analysis of cag pathogenicity island diversity in helicobacter pylori isolated from multi-ethnic patients in Malaysia
title_fullStr Functional analysis of cag pathogenicity island diversity in helicobacter pylori isolated from multi-ethnic patients in Malaysia
title_full_unstemmed Functional analysis of cag pathogenicity island diversity in helicobacter pylori isolated from multi-ethnic patients in Malaysia
title_sort functional analysis of cag pathogenicity island diversity in helicobacter pylori isolated from multi-ethnic patients in malaysia
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2024
url http://journalarticle.ukm.my/23745/1/SMT%2010.pdf
http://journalarticle.ukm.my/23745/
https://www.ukm.my/jsm/english_journals/vol53num3_2024/contentsVol53num3_2024.html
_version_ 1804066003651395584
score 13.211869

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