Cover Image

Metabolomics approach using LC-Orbitrap high resolution mass spectrometry and chemometrics for authentication of beef meats from different origins in Indonesia

Beef is one of the favourite meats consumed by people worldwide due to its high nutrition value needed by human development. It is highly susceptible for the adulteration practice by substituting beef with lower price meats by unethical meat traders due to the economic reasons. Therefore, the authen...

Full description

Saved in:
Bibliographic Details
Main Authors: Anjar Windarsih,, Abdul Rohman,, Nor Kartini Abu Bakar,, Erwanto, Yuny
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2023
Online Access:http://journalarticle.ukm.my/23332/1/SS%2011.pdf
http://journalarticle.ukm.my/23332/
https://www.ukm.my/jsm/english_journals/vol52num10_2023/contentsVol52num10_2023.html
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-ukm.journal.23332
record_format eprints
spelling my-ukm.journal.233322024-04-04T02:24:35Z http://journalarticle.ukm.my/23332/ Metabolomics approach using LC-Orbitrap high resolution mass spectrometry and chemometrics for authentication of beef meats from different origins in Indonesia Anjar Windarsih, Abdul Rohman, Nor Kartini Abu Bakar, Erwanto, Yuny Beef is one of the favourite meats consumed by people worldwide due to its high nutrition value needed by human development. It is highly susceptible for the adulteration practice by substituting beef with lower price meats by unethical meat traders due to the economic reasons. Therefore, the authenticity of beef meat (BM) is important because it is also related to halal status of meat which is required for certain religions. This research aimed to differentiate metabolites of BM from different origins using liquid chromatography-high resolution mass spectrometry (LC-HRMS) combined with chemometrics for the authentication purposes. Various metabolites mostly amino acids and lipids could be detected using methanol extraction. Principal component analysis (PCA), partial least square-discriminant analysis (PLS-DA) and sparse PLS-DA were successfully used to discriminate BMs from different origins. Fifty potential metabolite markers which are important for discrimination have been identified using variable importance projection (VIP) value extracted from PLS-DA analysis. Metabolites of (4S)-4-{[(9Z)-3-Hydroxy-9-hexadecenoyl] oxy}-4-(trimethylammonio)butanoate, N,N-Diisopropylethylamine (DIPEA), D-sphingosine, (2E,4Z)-N-Isobutyl- 2,4-octadecadienamide, 1-(14-methylhexadecanoyl)pyrrolidine, linoleic acid, 12-HAS, dodecylamine1, myristamide, and tributyl phosphate had high responsibility in discriminating BMs from different origins (VIP value > 2.0). It can be concluded that LC-HRMS based untargeted metabolomics combined with chemometrics could be used for authentication of BMs from different regions. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/23332/1/SS%2011.pdf Anjar Windarsih, and Abdul Rohman, and Nor Kartini Abu Bakar, and Erwanto, Yuny (2023) Metabolomics approach using LC-Orbitrap high resolution mass spectrometry and chemometrics for authentication of beef meats from different origins in Indonesia. Sains Malaysiana, 52 (10). pp. 2869-2887. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol52num10_2023/contentsVol52num10_2023.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Beef is one of the favourite meats consumed by people worldwide due to its high nutrition value needed by human development. It is highly susceptible for the adulteration practice by substituting beef with lower price meats by unethical meat traders due to the economic reasons. Therefore, the authenticity of beef meat (BM) is important because it is also related to halal status of meat which is required for certain religions. This research aimed to differentiate metabolites of BM from different origins using liquid chromatography-high resolution mass spectrometry (LC-HRMS) combined with chemometrics for the authentication purposes. Various metabolites mostly amino acids and lipids could be detected using methanol extraction. Principal component analysis (PCA), partial least square-discriminant analysis (PLS-DA) and sparse PLS-DA were successfully used to discriminate BMs from different origins. Fifty potential metabolite markers which are important for discrimination have been identified using variable importance projection (VIP) value extracted from PLS-DA analysis. Metabolites of (4S)-4-{[(9Z)-3-Hydroxy-9-hexadecenoyl] oxy}-4-(trimethylammonio)butanoate, N,N-Diisopropylethylamine (DIPEA), D-sphingosine, (2E,4Z)-N-Isobutyl- 2,4-octadecadienamide, 1-(14-methylhexadecanoyl)pyrrolidine, linoleic acid, 12-HAS, dodecylamine1, myristamide, and tributyl phosphate had high responsibility in discriminating BMs from different origins (VIP value > 2.0). It can be concluded that LC-HRMS based untargeted metabolomics combined with chemometrics could be used for authentication of BMs from different regions.
format Article
author Anjar Windarsih,
Abdul Rohman,
Nor Kartini Abu Bakar,
Erwanto, Yuny
spellingShingle Anjar Windarsih,
Abdul Rohman,
Nor Kartini Abu Bakar,
Erwanto, Yuny
Metabolomics approach using LC-Orbitrap high resolution mass spectrometry and chemometrics for authentication of beef meats from different origins in Indonesia
author_facet Anjar Windarsih,
Abdul Rohman,
Nor Kartini Abu Bakar,
Erwanto, Yuny
author_sort Anjar Windarsih,
title Metabolomics approach using LC-Orbitrap high resolution mass spectrometry and chemometrics for authentication of beef meats from different origins in Indonesia
title_short Metabolomics approach using LC-Orbitrap high resolution mass spectrometry and chemometrics for authentication of beef meats from different origins in Indonesia
title_full Metabolomics approach using LC-Orbitrap high resolution mass spectrometry and chemometrics for authentication of beef meats from different origins in Indonesia
title_fullStr Metabolomics approach using LC-Orbitrap high resolution mass spectrometry and chemometrics for authentication of beef meats from different origins in Indonesia
title_full_unstemmed Metabolomics approach using LC-Orbitrap high resolution mass spectrometry and chemometrics for authentication of beef meats from different origins in Indonesia
title_sort metabolomics approach using lc-orbitrap high resolution mass spectrometry and chemometrics for authentication of beef meats from different origins in indonesia
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2023
url http://journalarticle.ukm.my/23332/1/SS%2011.pdf
http://journalarticle.ukm.my/23332/
https://www.ukm.my/jsm/english_journals/vol52num10_2023/contentsVol52num10_2023.html
_version_ 1797908402221875200
score 13.211869

Similar Items