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The effects of light and a combination of growth regulators on the induction of somatic embryogenesis in orchid Rhynchostylis gigantea (LindI.) Ridl.

The squirrel-tail orchid (Rhynchostylis gigantea) belongs to the Orchidaceae family. This orchid is indigenous to Southeast Asia and is scented and arranged in a bouquet of dangling stems. Its uniqueness makes it commercially desirable. In vitro culture has been utilized for orchid proliferation for...

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Main Authors: Sri Rianawati,, Dasumiati,, Rahmi, Nur Amelia, Kurniati, Ridho Kurniati, Mulyaningsih, Enung Sri
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2023
Online Access:http://journalarticle.ukm.my/23242/1/SB%206.pdf
http://journalarticle.ukm.my/23242/
https://www.ukm.my/jsm/english_journals/vol52num11_2023/contentsVol52num11_2023.html
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spelling my-ukm.journal.232422024-03-25T01:37:53Z http://journalarticle.ukm.my/23242/ The effects of light and a combination of growth regulators on the induction of somatic embryogenesis in orchid Rhynchostylis gigantea (LindI.) Ridl. Sri Rianawati, Dasumiati, Rahmi, Nur Amelia Kurniati, Ridho Kurniati Mulyaningsih, Enung Sri The squirrel-tail orchid (Rhynchostylis gigantea) belongs to the Orchidaceae family. This orchid is indigenous to Southeast Asia and is scented and arranged in a bouquet of dangling stems. Its uniqueness makes it commercially desirable. In vitro culture has been utilized for orchid proliferation for a very long time to aid in the development of orchid seedlings. However, not all orchid species react the same way. Through somatic embryogenesis without gamete fusion, in vitro culture techniques can produce new plants during the entire embryonic phase. Endogenous and exogenous hormones, as well as light, influence the success of embryogenesis. Determining the optimal environmental parameters (light, combination and concentration of growth regulators, and their interaction) for inducing somatic embryogenesis from leaf explants in R. gigantea is the purpose of the present study. The leaves of R. gigantea clone 19 were utilized as explants in this investigation, utilizing a completely random factorial design. The first factor is a combination of growth regulator types and concentrations, and the second factor is light. Explants that are alive and growing at a rapid rate indicate that the in vitro culture is successful. Light and the combination of growth regulators significantly affected the percentage of viable explants, the beginning date of callus formation, and the number of embryogenic calluses produced. The interaction of two components (light and a combination of growth regulators) did not affect the three characteristics, with the exception of the proportion of somatic embryogenesis. Incubation in the dark is the optimal environment for initiating somatic embryogenesis in explants. The optimal combination of plant growth regulators for inducing somatic embryogenesis was 0.5 mgL-1 TDZ and 0.1 mgL-1 BAP. Bright light and a concentration of 1.0 mgL-1 TDZ + 0.1 mgL-1 BAP were the optimal interaction conditions for the induction of somatic embryogenesis in R. gigantea. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/23242/1/SB%206.pdf Sri Rianawati, and Dasumiati, and Rahmi, Nur Amelia and Kurniati, Ridho Kurniati and Mulyaningsih, Enung Sri (2023) The effects of light and a combination of growth regulators on the induction of somatic embryogenesis in orchid Rhynchostylis gigantea (LindI.) Ridl. Sains Malaysiana, 52 (11). pp. 3091-3102. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol52num11_2023/contentsVol52num11_2023.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description The squirrel-tail orchid (Rhynchostylis gigantea) belongs to the Orchidaceae family. This orchid is indigenous to Southeast Asia and is scented and arranged in a bouquet of dangling stems. Its uniqueness makes it commercially desirable. In vitro culture has been utilized for orchid proliferation for a very long time to aid in the development of orchid seedlings. However, not all orchid species react the same way. Through somatic embryogenesis without gamete fusion, in vitro culture techniques can produce new plants during the entire embryonic phase. Endogenous and exogenous hormones, as well as light, influence the success of embryogenesis. Determining the optimal environmental parameters (light, combination and concentration of growth regulators, and their interaction) for inducing somatic embryogenesis from leaf explants in R. gigantea is the purpose of the present study. The leaves of R. gigantea clone 19 were utilized as explants in this investigation, utilizing a completely random factorial design. The first factor is a combination of growth regulator types and concentrations, and the second factor is light. Explants that are alive and growing at a rapid rate indicate that the in vitro culture is successful. Light and the combination of growth regulators significantly affected the percentage of viable explants, the beginning date of callus formation, and the number of embryogenic calluses produced. The interaction of two components (light and a combination of growth regulators) did not affect the three characteristics, with the exception of the proportion of somatic embryogenesis. Incubation in the dark is the optimal environment for initiating somatic embryogenesis in explants. The optimal combination of plant growth regulators for inducing somatic embryogenesis was 0.5 mgL-1 TDZ and 0.1 mgL-1 BAP. Bright light and a concentration of 1.0 mgL-1 TDZ + 0.1 mgL-1 BAP were the optimal interaction conditions for the induction of somatic embryogenesis in R. gigantea.
format Article
author Sri Rianawati,
Dasumiati,
Rahmi, Nur Amelia
Kurniati, Ridho Kurniati
Mulyaningsih, Enung Sri
spellingShingle Sri Rianawati,
Dasumiati,
Rahmi, Nur Amelia
Kurniati, Ridho Kurniati
Mulyaningsih, Enung Sri
The effects of light and a combination of growth regulators on the induction of somatic embryogenesis in orchid Rhynchostylis gigantea (LindI.) Ridl.
author_facet Sri Rianawati,
Dasumiati,
Rahmi, Nur Amelia
Kurniati, Ridho Kurniati
Mulyaningsih, Enung Sri
author_sort Sri Rianawati,
title The effects of light and a combination of growth regulators on the induction of somatic embryogenesis in orchid Rhynchostylis gigantea (LindI.) Ridl.
title_short The effects of light and a combination of growth regulators on the induction of somatic embryogenesis in orchid Rhynchostylis gigantea (LindI.) Ridl.
title_full The effects of light and a combination of growth regulators on the induction of somatic embryogenesis in orchid Rhynchostylis gigantea (LindI.) Ridl.
title_fullStr The effects of light and a combination of growth regulators on the induction of somatic embryogenesis in orchid Rhynchostylis gigantea (LindI.) Ridl.
title_full_unstemmed The effects of light and a combination of growth regulators on the induction of somatic embryogenesis in orchid Rhynchostylis gigantea (LindI.) Ridl.
title_sort effects of light and a combination of growth regulators on the induction of somatic embryogenesis in orchid rhynchostylis gigantea (lindi.) ridl.
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2023
url http://journalarticle.ukm.my/23242/1/SB%206.pdf
http://journalarticle.ukm.my/23242/
https://www.ukm.my/jsm/english_journals/vol52num11_2023/contentsVol52num11_2023.html
_version_ 1794552830318608384
score 13.211869

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