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Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production

Green leaf volatiles (GLVs) play an essential role in plant defence, plant-plant interaction and plant-insect interaction. The plant releases GLVs and inhibits the growth and propagation of plant pathogens. In this study, overexpression of PmF-box1 in wild type A. thaliana showed the downregulation...

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Main Authors: Nur-Athirah Abd-Hamid,, Muhammad Naeem-Ul-Hassan,, Zamri Zainal,, Ismanizan Ismail,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2023
Online Access:http://journalarticle.ukm.my/22784/1/SEN%204.pdf
http://journalarticle.ukm.my/22784/
https://www.ukm.my/jsm/english_journals/vol52num6_2023/contentsVol52num6_2023.html
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spelling my-ukm.journal.227842024-01-03T02:29:05Z http://journalarticle.ukm.my/22784/ Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production Nur-Athirah Abd-Hamid, Muhammad Naeem-Ul-Hassan, Zamri Zainal, Ismanizan Ismail, Green leaf volatiles (GLVs) play an essential role in plant defence, plant-plant interaction and plant-insect interaction. The plant releases GLVs and inhibits the growth and propagation of plant pathogens. In this study, overexpression of PmF-box1 in wild type A. thaliana showed the downregulation of genes involved in the lipoxygenase-hydroperoxide lyase (LOX-HPL) pathway, which contributes to the biosynthesis of GLVs. It resulted in a marked reduction of hexanal production in the PmF-box1-overexpressing plant. The expression pattern of LOX-HPL branch genes in the kelch-repeat modified PmF-box1 (KMF)-overexpressing plant showed a pattern much closer to the expression of LOX-HPL branch genes in the vector control (VC) plant. It was shown that the functional KMF protein sequence was not responsible for the significant reduction of all GLVs including hexanal, 1-hexanol, (Z)-3-hexen-1-ol, and the carbon 5 (C5) volatile, 1-penten-3-ol, in plants overexpressing KMF. Furthermore, this study also showed that the relative proportion of production of 1-penten-3-ol to hexanal was higher in the PmF-box1-overexpressing plant. Based on the current comparative literature search, PmF-box1 does not appear to interact directly with the proteins or transcription factors of the LOX-HPL pathway. On the other hand, PmF-box1 interacts with SAMS1, which subsequently influences the HPL pathway enzyme genes. Thus, this study highlights the potential roles of PmF-box1 in the manipulation of GLV productions. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/22784/1/SEN%204.pdf Nur-Athirah Abd-Hamid, and Muhammad Naeem-Ul-Hassan, and Zamri Zainal, and Ismanizan Ismail, (2023) Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production. Sains Malaysiana, 52 (6). pp. 1649-1670. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol52num6_2023/contentsVol52num6_2023.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Green leaf volatiles (GLVs) play an essential role in plant defence, plant-plant interaction and plant-insect interaction. The plant releases GLVs and inhibits the growth and propagation of plant pathogens. In this study, overexpression of PmF-box1 in wild type A. thaliana showed the downregulation of genes involved in the lipoxygenase-hydroperoxide lyase (LOX-HPL) pathway, which contributes to the biosynthesis of GLVs. It resulted in a marked reduction of hexanal production in the PmF-box1-overexpressing plant. The expression pattern of LOX-HPL branch genes in the kelch-repeat modified PmF-box1 (KMF)-overexpressing plant showed a pattern much closer to the expression of LOX-HPL branch genes in the vector control (VC) plant. It was shown that the functional KMF protein sequence was not responsible for the significant reduction of all GLVs including hexanal, 1-hexanol, (Z)-3-hexen-1-ol, and the carbon 5 (C5) volatile, 1-penten-3-ol, in plants overexpressing KMF. Furthermore, this study also showed that the relative proportion of production of 1-penten-3-ol to hexanal was higher in the PmF-box1-overexpressing plant. Based on the current comparative literature search, PmF-box1 does not appear to interact directly with the proteins or transcription factors of the LOX-HPL pathway. On the other hand, PmF-box1 interacts with SAMS1, which subsequently influences the HPL pathway enzyme genes. Thus, this study highlights the potential roles of PmF-box1 in the manipulation of GLV productions.
format Article
author Nur-Athirah Abd-Hamid,
Muhammad Naeem-Ul-Hassan,
Zamri Zainal,
Ismanizan Ismail,
spellingShingle Nur-Athirah Abd-Hamid,
Muhammad Naeem-Ul-Hassan,
Zamri Zainal,
Ismanizan Ismail,
Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production
author_facet Nur-Athirah Abd-Hamid,
Muhammad Naeem-Ul-Hassan,
Zamri Zainal,
Ismanizan Ismail,
author_sort Nur-Athirah Abd-Hamid,
title Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production
title_short Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production
title_full Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production
title_fullStr Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production
title_full_unstemmed Persicaria minor F-box gene PmF-box1 indirectly affects Arabidopsis thaliana LOX-HPL pathway for green leaf volatile production
title_sort persicaria minor f-box gene pmf-box1 indirectly affects arabidopsis thaliana lox-hpl pathway for green leaf volatile production
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2023
url http://journalarticle.ukm.my/22784/1/SEN%204.pdf
http://journalarticle.ukm.my/22784/
https://www.ukm.my/jsm/english_journals/vol52num6_2023/contentsVol52num6_2023.html
_version_ 1787134664919482368
score 13.211869

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