Cover Image

Constitutive expression of cyclotide kalata B1 gene in transgenic rice conferring resistance to golden apple snail (Pomacea canaliculata)

The golden apple snail, also known as Siput Gondang Emas in Malaysia, is a serious pest of paddy fields and native aquatic plants throughout Southeast Asia. Agrobacterium-mediated transformation was used to transform a synthetic Oak 1 gene encoding kalata B1 (kB1), which is toxic to golden apple sna...

Full description

Saved in:
Bibliographic Details
Main Authors: Norsharina Md Saad,, Chee, How Teo, Zuraida Ab Rahman,, Zamri Zainal,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2023
Online Access:http://journalarticle.ukm.my/22509/1/MAS%207.pdf
http://journalarticle.ukm.my/22509/
https://jms.mabjournal.com/index.php/mab/index
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-ukm.journal.22509
record_format eprints
spelling my-ukm.journal.225092023-11-23T06:21:56Z http://journalarticle.ukm.my/22509/ Constitutive expression of cyclotide kalata B1 gene in transgenic rice conferring resistance to golden apple snail (Pomacea canaliculata) Norsharina Md Saad, Chee, How Teo Zuraida Ab Rahman, Zamri Zainal, The golden apple snail, also known as Siput Gondang Emas in Malaysia, is a serious pest of paddy fields and native aquatic plants throughout Southeast Asia. Agrobacterium-mediated transformation was used to transform a synthetic Oak 1 gene encoding kalata B1 (kB1), which is toxic to golden apple snails, into Malaysian indica rice MR219. The synthetic Oak 1 gene was placed under the control of a strong constitutive Zea mays ubiquitin promoter. Twelve transgenic lines containing the Oak 1 gene were obtained from the regenerated calli selected on hygromycin. Oak 1 gene expression was determined using quantitative reverse transcriptase- PCR (RT-qPCR). The resistance of the transgenic line to snail infestation was evaluated by feeding experiments. One dimensional 1H nuclear magnetic resonance (NMR) spectroscopy revealed that the kB1 produced in transgenic rice is in the form of an acyclic peptide. Phenotypic analysis of the transgenic plants revealed that they have fewer leaves and grains than wild-type MR219. In a molluscicidal activity bioassay, feeding juvenile snails with different concentrations of leaf extracts resulted in molluscicidal activity against snails that was comparable to the synthetic molluscicide metaldehyde, thus farmers can overcome the golden apple snail infestation problem by using genetically modified rice containing the kB1-encoding gene. This technology also has the potential to reduce the toxic effects of chemically synthesized molluscicides on the environment and ecosystem. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/22509/1/MAS%207.pdf Norsharina Md Saad, and Chee, How Teo and Zuraida Ab Rahman, and Zamri Zainal, (2023) Constitutive expression of cyclotide kalata B1 gene in transgenic rice conferring resistance to golden apple snail (Pomacea canaliculata). Malaysian Applied Biology, 52 (3). pp. 59-72. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description The golden apple snail, also known as Siput Gondang Emas in Malaysia, is a serious pest of paddy fields and native aquatic plants throughout Southeast Asia. Agrobacterium-mediated transformation was used to transform a synthetic Oak 1 gene encoding kalata B1 (kB1), which is toxic to golden apple snails, into Malaysian indica rice MR219. The synthetic Oak 1 gene was placed under the control of a strong constitutive Zea mays ubiquitin promoter. Twelve transgenic lines containing the Oak 1 gene were obtained from the regenerated calli selected on hygromycin. Oak 1 gene expression was determined using quantitative reverse transcriptase- PCR (RT-qPCR). The resistance of the transgenic line to snail infestation was evaluated by feeding experiments. One dimensional 1H nuclear magnetic resonance (NMR) spectroscopy revealed that the kB1 produced in transgenic rice is in the form of an acyclic peptide. Phenotypic analysis of the transgenic plants revealed that they have fewer leaves and grains than wild-type MR219. In a molluscicidal activity bioassay, feeding juvenile snails with different concentrations of leaf extracts resulted in molluscicidal activity against snails that was comparable to the synthetic molluscicide metaldehyde, thus farmers can overcome the golden apple snail infestation problem by using genetically modified rice containing the kB1-encoding gene. This technology also has the potential to reduce the toxic effects of chemically synthesized molluscicides on the environment and ecosystem.
format Article
author Norsharina Md Saad,
Chee, How Teo
Zuraida Ab Rahman,
Zamri Zainal,
spellingShingle Norsharina Md Saad,
Chee, How Teo
Zuraida Ab Rahman,
Zamri Zainal,
Constitutive expression of cyclotide kalata B1 gene in transgenic rice conferring resistance to golden apple snail (Pomacea canaliculata)
author_facet Norsharina Md Saad,
Chee, How Teo
Zuraida Ab Rahman,
Zamri Zainal,
author_sort Norsharina Md Saad,
title Constitutive expression of cyclotide kalata B1 gene in transgenic rice conferring resistance to golden apple snail (Pomacea canaliculata)
title_short Constitutive expression of cyclotide kalata B1 gene in transgenic rice conferring resistance to golden apple snail (Pomacea canaliculata)
title_full Constitutive expression of cyclotide kalata B1 gene in transgenic rice conferring resistance to golden apple snail (Pomacea canaliculata)
title_fullStr Constitutive expression of cyclotide kalata B1 gene in transgenic rice conferring resistance to golden apple snail (Pomacea canaliculata)
title_full_unstemmed Constitutive expression of cyclotide kalata B1 gene in transgenic rice conferring resistance to golden apple snail (Pomacea canaliculata)
title_sort constitutive expression of cyclotide kalata b1 gene in transgenic rice conferring resistance to golden apple snail (pomacea canaliculata)
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2023
url http://journalarticle.ukm.my/22509/1/MAS%207.pdf
http://journalarticle.ukm.my/22509/
https://jms.mabjournal.com/index.php/mab/index
_version_ 1783877710294024192
score 13.211869

Similar Items