Staff View: mIR-99a-5p and mIR-148a-3p as candidate molecular biomarkers for the survival of lung cancer patients

mIR-99a-5p and mIR-148a-3p as candidate molecular biomarkers for the survival of lung cancer patients

MicroRNA (miRNA) has emerged as a promising biomarker for improving the current state of an early lung cancer diagnosis. Multiple studies have reported that circulating miRNAs are usually combined in a single panel to determine lung cancer risk. In this study, we sought to assess the prognostic pred...

Full description

Saved in:

Bibliographic Details
Main Authors:	Muhammad-Redha Abdullah-Zawawi,, Mira-Farzana Mohamad-Mokhtar,, Saiful Effendi Syafruddin,, Fateen Farhana Ibrahim,, Isa Mohamed Rose,, Roslan Harun,, Nor Azian Abdul Murad,
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2023
Online Access:	http://journalarticle.ukm.my/22456/1/JM%207.pdf http://journalarticle.ukm.my/22456/ https://jms.mabjournal.com/index.php/mab/index
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-ukm.journal.22456
record_format	eprints
spelling	my-ukm.journal.224562023-11-14T00:56:07Z http://journalarticle.ukm.my/22456/ mIR-99a-5p and mIR-148a-3p as candidate molecular biomarkers for the survival of lung cancer patients Muhammad-Redha Abdullah-Zawawi, Mira-Farzana Mohamad-Mokhtar, Saiful Effendi Syafruddin, Fateen Farhana Ibrahim, Isa Mohamed Rose, Roslan Harun, Nor Azian Abdul Murad, MicroRNA (miRNA) has emerged as a promising biomarker for improving the current state of an early lung cancer diagnosis. Multiple studies have reported that circulating miRNAs are usually combined in a single panel to determine lung cancer risk. In this study, we sought to assess the prognostic predictive values of the potential miRNAs for lung cancer survival among Malaysian patients. The microarray analysis was performed on the isolated miRNA samples of formalin-fixed lung cancer tissues from Malaysian populations. The correlation between miRNA expression and lung adenocarcinoma (LUAD) patient survival was predicted using TGGA data, followed by extensive in silico analyses, including miRNA target gene identification, protein-protein interaction (PPI) network construction, subnetwork (SN) detection, functional enrichment analysis, gene-disease associations, and survival analysis in advanced-stage LUAD. Overall, two promising miR-99a-5p and miR-148a-3p were upregulated in the patients with good survival. We found that 64 miR-99a-5p and 95 miR-148a-3p target genes were associated with poor prognosis and highly participated in cancer-associated processes, such as apoptosis, mRNA transport and cell-cell adhesion. The density score of 4.667, 3.333, and 3.000 in respective SN1, SN2, and SN3 showed the significant subnetworks of constructed PPI leading to the identification of 17 targets, of which ~79% of them involved in neoplastic diseases. Four high-confidence target genes (SUDS3, TOMM22, KPNA4, and HMGB1) were associated with worse overall survival in LUAD patients, implying their critical roles in LUAD pathogenesis. These findings shed additional light on the roles of miR-99a-5p and miR-148a-3p as potential biomarkers for LUAD survival. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/22456/1/JM%207.pdf Muhammad-Redha Abdullah-Zawawi, and Mira-Farzana Mohamad-Mokhtar, and Saiful Effendi Syafruddin, and Fateen Farhana Ibrahim, and Isa Mohamed Rose, and Roslan Harun, and Nor Azian Abdul Murad, (2023) mIR-99a-5p and mIR-148a-3p as candidate molecular biomarkers for the survival of lung cancer patients. Malaysian Applied Biology, 52 (1). pp. 87-100. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index
institution	Universiti Kebangsaan Malaysia
building	Tun Sri Lanang Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Kebangsaan Malaysia
content_source	UKM Journal Article Repository
url_provider	http://journalarticle.ukm.my/
language	English
description	MicroRNA (miRNA) has emerged as a promising biomarker for improving the current state of an early lung cancer diagnosis. Multiple studies have reported that circulating miRNAs are usually combined in a single panel to determine lung cancer risk. In this study, we sought to assess the prognostic predictive values of the potential miRNAs for lung cancer survival among Malaysian patients. The microarray analysis was performed on the isolated miRNA samples of formalin-fixed lung cancer tissues from Malaysian populations. The correlation between miRNA expression and lung adenocarcinoma (LUAD) patient survival was predicted using TGGA data, followed by extensive in silico analyses, including miRNA target gene identification, protein-protein interaction (PPI) network construction, subnetwork (SN) detection, functional enrichment analysis, gene-disease associations, and survival analysis in advanced-stage LUAD. Overall, two promising miR-99a-5p and miR-148a-3p were upregulated in the patients with good survival. We found that 64 miR-99a-5p and 95 miR-148a-3p target genes were associated with poor prognosis and highly participated in cancer-associated processes, such as apoptosis, mRNA transport and cell-cell adhesion. The density score of 4.667, 3.333, and 3.000 in respective SN1, SN2, and SN3 showed the significant subnetworks of constructed PPI leading to the identification of 17 targets, of which ~79% of them involved in neoplastic diseases. Four high-confidence target genes (SUDS3, TOMM22, KPNA4, and HMGB1) were associated with worse overall survival in LUAD patients, implying their critical roles in LUAD pathogenesis. These findings shed additional light on the roles of miR-99a-5p and miR-148a-3p as potential biomarkers for LUAD survival.
format	Article
author	Muhammad-Redha Abdullah-Zawawi, Mira-Farzana Mohamad-Mokhtar, Saiful Effendi Syafruddin, Fateen Farhana Ibrahim, Isa Mohamed Rose, Roslan Harun, Nor Azian Abdul Murad,
spellingShingle	Muhammad-Redha Abdullah-Zawawi, Mira-Farzana Mohamad-Mokhtar, Saiful Effendi Syafruddin, Fateen Farhana Ibrahim, Isa Mohamed Rose, Roslan Harun, Nor Azian Abdul Murad, mIR-99a-5p and mIR-148a-3p as candidate molecular biomarkers for the survival of lung cancer patients
author_facet	Muhammad-Redha Abdullah-Zawawi, Mira-Farzana Mohamad-Mokhtar, Saiful Effendi Syafruddin, Fateen Farhana Ibrahim, Isa Mohamed Rose, Roslan Harun, Nor Azian Abdul Murad,
author_sort	Muhammad-Redha Abdullah-Zawawi,
title	mIR-99a-5p and mIR-148a-3p as candidate molecular biomarkers for the survival of lung cancer patients
title_short	mIR-99a-5p and mIR-148a-3p as candidate molecular biomarkers for the survival of lung cancer patients
title_full	mIR-99a-5p and mIR-148a-3p as candidate molecular biomarkers for the survival of lung cancer patients
title_fullStr	mIR-99a-5p and mIR-148a-3p as candidate molecular biomarkers for the survival of lung cancer patients
title_full_unstemmed	mIR-99a-5p and mIR-148a-3p as candidate molecular biomarkers for the survival of lung cancer patients
title_sort	mir-99a-5p and mir-148a-3p as candidate molecular biomarkers for the survival of lung cancer patients
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2023
url	http://journalarticle.ukm.my/22456/1/JM%207.pdf http://journalarticle.ukm.my/22456/ https://jms.mabjournal.com/index.php/mab/index
_version_	1783877703530708992
score	13.226497

mIR-99a-5p and mIR-148a-3p as candidate molecular biomarkers for the survival of lung cancer patients

Similar Items