Cover Image

Detection of Leptospira wolffii in water and soil on livestock farms in Kelantan after a massive flood

Pathogenic leptospires cause leptospirosis in both animals and humans around the world. Humans can be infected by leptospires through direct contact with infected animals or indirect contact with leptospires contaminated water or soil. Outbreaks of leptospirosis usually occur after heavy rainfall an...

Full description

Saved in:
Bibliographic Details
Main Authors: Mohammad Sabri Abdul Rahman,, Siti Khairani Bejo,, Zunita Zakaria,, Latiffah Hassan,, Mohd Azri Roslan,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2023
Online Access:http://journalarticle.ukm.my/22150/1/SL%205.pdf
http://journalarticle.ukm.my/22150/
http://www.ukm.my/jsm/index.html
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-ukm.journal.22150
record_format eprints
spelling my-ukm.journal.221502023-09-08T07:42:30Z http://journalarticle.ukm.my/22150/ Detection of Leptospira wolffii in water and soil on livestock farms in Kelantan after a massive flood Mohammad Sabri Abdul Rahman, Siti Khairani Bejo, Zunita Zakaria, Latiffah Hassan, Mohd Azri Roslan, Pathogenic leptospires cause leptospirosis in both animals and humans around the world. Humans can be infected by leptospires through direct contact with infected animals or indirect contact with leptospires contaminated water or soil. Outbreaks of leptospirosis usually occur after heavy rainfall and flooding, particularly in endemic areas. The aim of this study was to detect the presence of leptospires in water and soil in livestock farms after a massive flood in Kelantan. Samples were collected from 28 livestock farms in Kelantan, comprising 62 water samples and 62 soil samples, respectively. The samples were filtered and inoculated into a semisolid EMJH medium containing 5-FU for the isolation of Leptospira spp. The Leptospira spp. isolates were then identified using classical methods (1M NaCl, 8-azaguanine, and 13 °C), serology (MAT), multiplex polymerase chain reaction (mPCR), and DNA sequencing. The classical identification methods showed varying results and failed to differentiate between pathogenic and non-pathogenic leptospires. MAT showed the isolates reactions against serovars Autumnalis, Hebdomadis, Pyrogenes, Bataviae, Patoc, and Wolffii. However, mPCR showed that all isolates were non-pathogenic Leptospira spp. Further identification using DNA sequencing found that all isolates were identified as Leptospira wolffii, an intermediate species of Leptospira. The presence of L. wolffii in water and soil provides evidence that this species is circulating in the environment and could potentially transmit to humans and animals. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/22150/1/SL%205.pdf Mohammad Sabri Abdul Rahman, and Siti Khairani Bejo, and Zunita Zakaria, and Latiffah Hassan, and Mohd Azri Roslan, (2023) Detection of Leptospira wolffii in water and soil on livestock farms in Kelantan after a massive flood. Sains Malaysiana, 52 (5). pp. 1383-1395. ISSN 0126-6039 http://www.ukm.my/jsm/index.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Pathogenic leptospires cause leptospirosis in both animals and humans around the world. Humans can be infected by leptospires through direct contact with infected animals or indirect contact with leptospires contaminated water or soil. Outbreaks of leptospirosis usually occur after heavy rainfall and flooding, particularly in endemic areas. The aim of this study was to detect the presence of leptospires in water and soil in livestock farms after a massive flood in Kelantan. Samples were collected from 28 livestock farms in Kelantan, comprising 62 water samples and 62 soil samples, respectively. The samples were filtered and inoculated into a semisolid EMJH medium containing 5-FU for the isolation of Leptospira spp. The Leptospira spp. isolates were then identified using classical methods (1M NaCl, 8-azaguanine, and 13 °C), serology (MAT), multiplex polymerase chain reaction (mPCR), and DNA sequencing. The classical identification methods showed varying results and failed to differentiate between pathogenic and non-pathogenic leptospires. MAT showed the isolates reactions against serovars Autumnalis, Hebdomadis, Pyrogenes, Bataviae, Patoc, and Wolffii. However, mPCR showed that all isolates were non-pathogenic Leptospira spp. Further identification using DNA sequencing found that all isolates were identified as Leptospira wolffii, an intermediate species of Leptospira. The presence of L. wolffii in water and soil provides evidence that this species is circulating in the environment and could potentially transmit to humans and animals.
format Article
author Mohammad Sabri Abdul Rahman,
Siti Khairani Bejo,
Zunita Zakaria,
Latiffah Hassan,
Mohd Azri Roslan,
spellingShingle Mohammad Sabri Abdul Rahman,
Siti Khairani Bejo,
Zunita Zakaria,
Latiffah Hassan,
Mohd Azri Roslan,
Detection of Leptospira wolffii in water and soil on livestock farms in Kelantan after a massive flood
author_facet Mohammad Sabri Abdul Rahman,
Siti Khairani Bejo,
Zunita Zakaria,
Latiffah Hassan,
Mohd Azri Roslan,
author_sort Mohammad Sabri Abdul Rahman,
title Detection of Leptospira wolffii in water and soil on livestock farms in Kelantan after a massive flood
title_short Detection of Leptospira wolffii in water and soil on livestock farms in Kelantan after a massive flood
title_full Detection of Leptospira wolffii in water and soil on livestock farms in Kelantan after a massive flood
title_fullStr Detection of Leptospira wolffii in water and soil on livestock farms in Kelantan after a massive flood
title_full_unstemmed Detection of Leptospira wolffii in water and soil on livestock farms in Kelantan after a massive flood
title_sort detection of leptospira wolffii in water and soil on livestock farms in kelantan after a massive flood
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2023
url http://journalarticle.ukm.my/22150/1/SL%205.pdf
http://journalarticle.ukm.my/22150/
http://www.ukm.my/jsm/index.html
_version_ 1778162561658650624
score 13.211869

Similar Items