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UHPLC-Q-Orbitrap HRMS-based metabolomic show biological pathways involved in rice (Oryza sativa L.) under fe toxicity stress

The iron (Fe) toxicity stress is still a serious problem in rice cultivation, especially on land with high Fe content. The Fe toxicity stress affects various complex physiological aspects of plants. The metabolomic analysis using LC-MS is expected to provide information about rice’s metabolism regul...

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Main Authors: Turhadi, Turhadi, Hamim, Hamim, Ghulamahdi, Munif, Miftahudin, Miftahudin
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2023
Online Access:http://journalarticle.ukm.my/21618/1/SD%2015.pdf
http://journalarticle.ukm.my/21618/
http://www.ukm.my/jsm/index.html
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spelling my-ukm.journal.216182023-05-23T03:01:44Z http://journalarticle.ukm.my/21618/ UHPLC-Q-Orbitrap HRMS-based metabolomic show biological pathways involved in rice (Oryza sativa L.) under fe toxicity stress Turhadi, Turhadi Hamim, Hamim Ghulamahdi, Munif Miftahudin, Miftahudin The iron (Fe) toxicity stress is still a serious problem in rice cultivation, especially on land with high Fe content. The Fe toxicity stress affects various complex physiological aspects of plants. The metabolomic analysis using LC-MS is expected to provide information about rice’s metabolism regulation under Fe toxicity stress. The objective of this study was to show the biological pathway signature in rice after exposure to Fe toxicity stress using UHPLC-Q-Orbitrap HRMS-based metabolomic analysis. The two rice varieties, i.e., IR64 (Fe-sensitive) and Pokkali (Fe-tolerant) were analyzed their metabolites using UHPLC-Q-Orbitrap HRMS. The metabolite profiles of both varieties were analyzed using MetaboAnalyst 5.0 software. The results showed that Fe toxicity stress affected the metabolite profile in both root and shoot tissues of two rice varieties. A number of 102 metabolites were detected in root and shoot tissues of rice. The comprehensive univariate and multivariate analyses showed that 1-aminocyclopropane-1-carboxylate (ACC) in shoot tissues and galactose in root tissues was suggested as metabolite markers for Fe tolerance character of rice var. Pokkali. The genes encoded the enzymes involved in biosynthetic pathway of both metabolite markers could be a target to be explored for Fe toxicity tolerance in rice. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/21618/1/SD%2015.pdf Turhadi, Turhadi and Hamim, Hamim and Ghulamahdi, Munif and Miftahudin, Miftahudin (2023) UHPLC-Q-Orbitrap HRMS-based metabolomic show biological pathways involved in rice (Oryza sativa L.) under fe toxicity stress. Sains Malaysiana, 52 (2). pp. 513-531. ISSN 0126-6039 http://www.ukm.my/jsm/index.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description The iron (Fe) toxicity stress is still a serious problem in rice cultivation, especially on land with high Fe content. The Fe toxicity stress affects various complex physiological aspects of plants. The metabolomic analysis using LC-MS is expected to provide information about rice’s metabolism regulation under Fe toxicity stress. The objective of this study was to show the biological pathway signature in rice after exposure to Fe toxicity stress using UHPLC-Q-Orbitrap HRMS-based metabolomic analysis. The two rice varieties, i.e., IR64 (Fe-sensitive) and Pokkali (Fe-tolerant) were analyzed their metabolites using UHPLC-Q-Orbitrap HRMS. The metabolite profiles of both varieties were analyzed using MetaboAnalyst 5.0 software. The results showed that Fe toxicity stress affected the metabolite profile in both root and shoot tissues of two rice varieties. A number of 102 metabolites were detected in root and shoot tissues of rice. The comprehensive univariate and multivariate analyses showed that 1-aminocyclopropane-1-carboxylate (ACC) in shoot tissues and galactose in root tissues was suggested as metabolite markers for Fe tolerance character of rice var. Pokkali. The genes encoded the enzymes involved in biosynthetic pathway of both metabolite markers could be a target to be explored for Fe toxicity tolerance in rice.
format Article
author Turhadi, Turhadi
Hamim, Hamim
Ghulamahdi, Munif
Miftahudin, Miftahudin
spellingShingle Turhadi, Turhadi
Hamim, Hamim
Ghulamahdi, Munif
Miftahudin, Miftahudin
UHPLC-Q-Orbitrap HRMS-based metabolomic show biological pathways involved in rice (Oryza sativa L.) under fe toxicity stress
author_facet Turhadi, Turhadi
Hamim, Hamim
Ghulamahdi, Munif
Miftahudin, Miftahudin
author_sort Turhadi, Turhadi
title UHPLC-Q-Orbitrap HRMS-based metabolomic show biological pathways involved in rice (Oryza sativa L.) under fe toxicity stress
title_short UHPLC-Q-Orbitrap HRMS-based metabolomic show biological pathways involved in rice (Oryza sativa L.) under fe toxicity stress
title_full UHPLC-Q-Orbitrap HRMS-based metabolomic show biological pathways involved in rice (Oryza sativa L.) under fe toxicity stress
title_fullStr UHPLC-Q-Orbitrap HRMS-based metabolomic show biological pathways involved in rice (Oryza sativa L.) under fe toxicity stress
title_full_unstemmed UHPLC-Q-Orbitrap HRMS-based metabolomic show biological pathways involved in rice (Oryza sativa L.) under fe toxicity stress
title_sort uhplc-q-orbitrap hrms-based metabolomic show biological pathways involved in rice (oryza sativa l.) under fe toxicity stress
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2023
url http://journalarticle.ukm.my/21618/1/SD%2015.pdf
http://journalarticle.ukm.my/21618/
http://www.ukm.my/jsm/index.html
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score 13.211869

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