Staff View: Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM)

Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM)

Fortunella polyandra (Ridl) Tanaka or Malayan kumquat, is a family of Rutaceae from Malaysia known for its various active compounds, especially flavonoids. This study aims to verify the optimum condition and correlation of leaves extract in Fortunella polyandra by ultrasound-assisted extraction (U...

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Main Authors:	Hazim Syahmi Elias,, Syarul Nataqain Baharum,, Kamalrul Azlan Azizan,, Wan Yaacob Wan Ahmad,, Sheela Chandren,, Norazah Basar,
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2023
Online Access:	http://journalarticle.ukm.my/21541/1/S%2012.pdf http://journalarticle.ukm.my/21541/ http://www.ukm.my/jsm/index.html
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id	my-ukm.journal.21541
record_format	eprints
spelling	my-ukm.journal.215412023-05-10T06:25:12Z http://journalarticle.ukm.my/21541/ Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM) Hazim Syahmi Elias, Syarul Nataqain Baharum, Kamalrul Azlan Azizan, Wan Yaacob Wan Ahmad, Sheela Chandren, Norazah Basar, Fortunella polyandra (Ridl) Tanaka or Malayan kumquat, is a family of Rutaceae from Malaysia known for its various active compounds, especially flavonoids. This study aims to verify the optimum condition and correlation of leaves extract in Fortunella polyandra by ultrasound-assisted extraction (UAE) for antioxidant activities and apigenin 7-O-neohesperidoside (rhoifolin) contents using the response surface methodology (RSM). Central composite rotatable design (CCD) was performed to investigate the influence of the extraction parameters on maximum antioxidant activity, namely temperatures (X1), times (X2), and solvent ratios of ethanol:water (X3). The optimal extraction conditions for 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were obtained at extraction time of 5 min, temperature of 30 °C and solvent ratio of 70:30, ethanol:water with IC50 value of 0.126±0.004 mg/mL. Rhoifolin content and ferric reducing antioxidant potential (FRAP) assay share the same optimum condition at extraction time of 5 min, temperature of 30 °C, and solvent ratio of 30:70, ethanol:water. Under these conditions,rhoifolin content was measured at 67.83±0.37 ppm, whereas FRAP equivalent value was recorded at 0.22±0.01 mg/mL. All three responses from the model achieved 95% confidence level. Correlation study showed that there is no relationship between the rhoifolin content and the antioxidant activities of Fortunella polyandra extract. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/21541/1/S%2012.pdf Hazim Syahmi Elias, and Syarul Nataqain Baharum, and Kamalrul Azlan Azizan, and Wan Yaacob Wan Ahmad, and Sheela Chandren, and Norazah Basar, (2023) Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM). Sains Malaysiana, 52 (1). pp. 153-164. ISSN 0126-6039 http://www.ukm.my/jsm/index.html
institution	Universiti Kebangsaan Malaysia
building	Tun Sri Lanang Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Kebangsaan Malaysia
content_source	UKM Journal Article Repository
url_provider	http://journalarticle.ukm.my/
language	English
description	Fortunella polyandra (Ridl) Tanaka or Malayan kumquat, is a family of Rutaceae from Malaysia known for its various active compounds, especially flavonoids. This study aims to verify the optimum condition and correlation of leaves extract in Fortunella polyandra by ultrasound-assisted extraction (UAE) for antioxidant activities and apigenin 7-O-neohesperidoside (rhoifolin) contents using the response surface methodology (RSM). Central composite rotatable design (CCD) was performed to investigate the influence of the extraction parameters on maximum antioxidant activity, namely temperatures (X1), times (X2), and solvent ratios of ethanol:water (X3). The optimal extraction conditions for 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were obtained at extraction time of 5 min, temperature of 30 °C and solvent ratio of 70:30, ethanol:water with IC50 value of 0.126±0.004 mg/mL. Rhoifolin content and ferric reducing antioxidant potential (FRAP) assay share the same optimum condition at extraction time of 5 min, temperature of 30 °C, and solvent ratio of 30:70, ethanol:water. Under these conditions,rhoifolin content was measured at 67.83±0.37 ppm, whereas FRAP equivalent value was recorded at 0.22±0.01 mg/mL. All three responses from the model achieved 95% confidence level. Correlation study showed that there is no relationship between the rhoifolin content and the antioxidant activities of Fortunella polyandra extract.
format	Article
author	Hazim Syahmi Elias, Syarul Nataqain Baharum, Kamalrul Azlan Azizan, Wan Yaacob Wan Ahmad, Sheela Chandren, Norazah Basar,
spellingShingle	Hazim Syahmi Elias, Syarul Nataqain Baharum, Kamalrul Azlan Azizan, Wan Yaacob Wan Ahmad, Sheela Chandren, Norazah Basar, Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM)
author_facet	Hazim Syahmi Elias, Syarul Nataqain Baharum, Kamalrul Azlan Azizan, Wan Yaacob Wan Ahmad, Sheela Chandren, Norazah Basar,
author_sort	Hazim Syahmi Elias,
title	Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM)
title_short	Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM)
title_full	Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM)
title_fullStr	Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM)
title_full_unstemmed	Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM)
title_sort	optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of fortunella polyandra using response surface methodology (rsm)
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2023
url	http://journalarticle.ukm.my/21541/1/S%2012.pdf http://journalarticle.ukm.my/21541/ http://www.ukm.my/jsm/index.html
_version_	1768008339620364288
score	13.211869

Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM)

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