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HDL and its subpopulation (HDL2 and HDL3) promote cholesterol transporters expression and attenuate inflammation in 3T3-L1 mature adipocytes induced by tumor necrosis factor-alpha

Obesity activates inflammation causing dysfunction of adipocytes. Increasing high-density lipoprotein (HDL) levels in obesity may be beneficial in overcoming this effect. However, not much data is available on the effects of HDL and its subpopulations in inflamed adipocytes. The objective of this st...

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Main Authors: Suhaila Abd Muid,, Remee Awang Jalil,, Noor Hanisa Harun,, Hapizah Mohd Nawawi,, Gabriele Ruth Anisah Froemming,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2022
Online Access:http://journalarticle.ukm.my/21433/2/M%2019.pdf
http://journalarticle.ukm.my/21433/
https://jms.mabjournal.com/index.php/mab/index
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spelling my-ukm.journal.214332023-04-06T07:26:39Z http://journalarticle.ukm.my/21433/ HDL and its subpopulation (HDL2 and HDL3) promote cholesterol transporters expression and attenuate inflammation in 3T3-L1 mature adipocytes induced by tumor necrosis factor-alpha Suhaila Abd Muid, Remee Awang Jalil, Noor Hanisa Harun, Hapizah Mohd Nawawi, Gabriele Ruth Anisah Froemming, Obesity activates inflammation causing dysfunction of adipocytes. Increasing high-density lipoprotein (HDL) levels in obesity may be beneficial in overcoming this effect. However, not much data is available on the effects of HDL and its subpopulations in inflamed adipocytes. The objective of this study was to investigate the effects of total HDL (tHDL) and the comparison between its subpopulations (HDL2 & HDL3) on protein and gene expression of cholesterol transporters, inflammation, and adipokines in TNF-α stimulated 3T3-L1 mature adipocytes. TNFα alone had lower adiponectin and higher protein and gene expression of IL-6 and NF-ĸβ (p65) compared to unstimulated adipocytes and these effects were attenuated by HDLs especially HDL3 (in most of the biomarkers). HDL and its subpopulation had higher cholesterol transporters expression in 3T3-L1 mature adipocytes induced by TNF-α compared to unstimulated cells. Increment of cholesterol transporters expression by HDL leads to reduce secretion of inflammatory markers [IL-6 & NF-kB (p65)] and visfatin and increases adiponectin secretion in the inflamed mature adipocytes. HDL exhibits beyond its reverse cholesterol transporter property by exhibiting anti-inflammatory effects thru the deactivation of NF-ĸβ (p65). This may contribute to reducing the progression of obesity-related complications. Penerbit Universiti Kebangsaan Malaysia 2022 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/21433/2/M%2019.pdf Suhaila Abd Muid, and Remee Awang Jalil, and Noor Hanisa Harun, and Hapizah Mohd Nawawi, and Gabriele Ruth Anisah Froemming, (2022) HDL and its subpopulation (HDL2 and HDL3) promote cholesterol transporters expression and attenuate inflammation in 3T3-L1 mature adipocytes induced by tumor necrosis factor-alpha. Malaysian Applied Biology, 51 (4). pp. 153-167. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Obesity activates inflammation causing dysfunction of adipocytes. Increasing high-density lipoprotein (HDL) levels in obesity may be beneficial in overcoming this effect. However, not much data is available on the effects of HDL and its subpopulations in inflamed adipocytes. The objective of this study was to investigate the effects of total HDL (tHDL) and the comparison between its subpopulations (HDL2 & HDL3) on protein and gene expression of cholesterol transporters, inflammation, and adipokines in TNF-α stimulated 3T3-L1 mature adipocytes. TNFα alone had lower adiponectin and higher protein and gene expression of IL-6 and NF-ĸβ (p65) compared to unstimulated adipocytes and these effects were attenuated by HDLs especially HDL3 (in most of the biomarkers). HDL and its subpopulation had higher cholesterol transporters expression in 3T3-L1 mature adipocytes induced by TNF-α compared to unstimulated cells. Increment of cholesterol transporters expression by HDL leads to reduce secretion of inflammatory markers [IL-6 & NF-kB (p65)] and visfatin and increases adiponectin secretion in the inflamed mature adipocytes. HDL exhibits beyond its reverse cholesterol transporter property by exhibiting anti-inflammatory effects thru the deactivation of NF-ĸβ (p65). This may contribute to reducing the progression of obesity-related complications.
format Article
author Suhaila Abd Muid,
Remee Awang Jalil,
Noor Hanisa Harun,
Hapizah Mohd Nawawi,
Gabriele Ruth Anisah Froemming,
spellingShingle Suhaila Abd Muid,
Remee Awang Jalil,
Noor Hanisa Harun,
Hapizah Mohd Nawawi,
Gabriele Ruth Anisah Froemming,
HDL and its subpopulation (HDL2 and HDL3) promote cholesterol transporters expression and attenuate inflammation in 3T3-L1 mature adipocytes induced by tumor necrosis factor-alpha
author_facet Suhaila Abd Muid,
Remee Awang Jalil,
Noor Hanisa Harun,
Hapizah Mohd Nawawi,
Gabriele Ruth Anisah Froemming,
author_sort Suhaila Abd Muid,
title HDL and its subpopulation (HDL2 and HDL3) promote cholesterol transporters expression and attenuate inflammation in 3T3-L1 mature adipocytes induced by tumor necrosis factor-alpha
title_short HDL and its subpopulation (HDL2 and HDL3) promote cholesterol transporters expression and attenuate inflammation in 3T3-L1 mature adipocytes induced by tumor necrosis factor-alpha
title_full HDL and its subpopulation (HDL2 and HDL3) promote cholesterol transporters expression and attenuate inflammation in 3T3-L1 mature adipocytes induced by tumor necrosis factor-alpha
title_fullStr HDL and its subpopulation (HDL2 and HDL3) promote cholesterol transporters expression and attenuate inflammation in 3T3-L1 mature adipocytes induced by tumor necrosis factor-alpha
title_full_unstemmed HDL and its subpopulation (HDL2 and HDL3) promote cholesterol transporters expression and attenuate inflammation in 3T3-L1 mature adipocytes induced by tumor necrosis factor-alpha
title_sort hdl and its subpopulation (hdl2 and hdl3) promote cholesterol transporters expression and attenuate inflammation in 3t3-l1 mature adipocytes induced by tumor necrosis factor-alpha
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2022
url http://journalarticle.ukm.my/21433/2/M%2019.pdf
http://journalarticle.ukm.my/21433/
https://jms.mabjournal.com/index.php/mab/index
_version_ 1762838509741146112
score 13.211869

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