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Epigenetic modifiers and minerals as tools to diversify secondary metabolite production in fungi

Secondary metabolite production of fungi can be modified by different approaches, including epigenetic modifiers, culture-dependent methods, and genomic-based methods. In this study, secondary metabolite production was explored in the presence of epigenetic modifiers and minerals using a microscale...

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Main Authors: Siti Hajar Sadiran,, Fatmawati Lambuk,, Siti Nur Sarah Zubir,, Suliman, Rasha Saad,, Weber, Jean-Frédéric Faizal
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2022
Online Access:http://journalarticle.ukm.my/21430/1/M%2016.pdf
http://journalarticle.ukm.my/21430/
https://jms.mabjournal.com/index.php/mab/index
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spelling my-ukm.journal.214302023-04-06T07:00:06Z http://journalarticle.ukm.my/21430/ Epigenetic modifiers and minerals as tools to diversify secondary metabolite production in fungi Siti Hajar Sadiran, Fatmawati Lambuk, Siti Nur Sarah Zubir, Suliman, Rasha Saad, Weber, Jean-Frédéric Faizal Secondary metabolite production of fungi can be modified by different approaches, including epigenetic modifiers, culture-dependent methods, and genomic-based methods. In this study, secondary metabolite production was explored in the presence of epigenetic modifiers and minerals using a microscale fermentation approach. Thirteen fungi originally isolated from mushrooms and soils were grown in 96-well microtiter plates (MTPs) using 70% of potato dextrose broth (PDB) with the addition of epigenetic modifiers and minerals in different combinations and concentrations. All cultures were fermented at 10 °C or 28 °C for 2, 3, or 5 weeks and extracted by solid phase extraction. The resulting extracts were subjected to high-performance liquid chromatography (HPLC) and the chromatograms were analyzed on a qualitative and quantitative basis. In addition, major secondary metabolites from four fungi were identified as penicillic acid, patulin, pseurotin A, and javanicin. Epigenetic modifiers and minerals induce significant changes in the profile of the secondary metabolites. Their usage combined with microscale fermentation provides a cost-efficient tool for exploring fungal secondary metabolism. Penerbit Universiti Kebangsaan Malaysia 2022 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/21430/1/M%2016.pdf Siti Hajar Sadiran, and Fatmawati Lambuk, and Siti Nur Sarah Zubir, and Suliman, Rasha Saad, and Weber, Jean-Frédéric Faizal (2022) Epigenetic modifiers and minerals as tools to diversify secondary metabolite production in fungi. Malaysian Applied Biology, 51 (4). pp. 127-136. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Secondary metabolite production of fungi can be modified by different approaches, including epigenetic modifiers, culture-dependent methods, and genomic-based methods. In this study, secondary metabolite production was explored in the presence of epigenetic modifiers and minerals using a microscale fermentation approach. Thirteen fungi originally isolated from mushrooms and soils were grown in 96-well microtiter plates (MTPs) using 70% of potato dextrose broth (PDB) with the addition of epigenetic modifiers and minerals in different combinations and concentrations. All cultures were fermented at 10 °C or 28 °C for 2, 3, or 5 weeks and extracted by solid phase extraction. The resulting extracts were subjected to high-performance liquid chromatography (HPLC) and the chromatograms were analyzed on a qualitative and quantitative basis. In addition, major secondary metabolites from four fungi were identified as penicillic acid, patulin, pseurotin A, and javanicin. Epigenetic modifiers and minerals induce significant changes in the profile of the secondary metabolites. Their usage combined with microscale fermentation provides a cost-efficient tool for exploring fungal secondary metabolism.
format Article
author Siti Hajar Sadiran,
Fatmawati Lambuk,
Siti Nur Sarah Zubir,
Suliman, Rasha Saad,
Weber, Jean-Frédéric Faizal
spellingShingle Siti Hajar Sadiran,
Fatmawati Lambuk,
Siti Nur Sarah Zubir,
Suliman, Rasha Saad,
Weber, Jean-Frédéric Faizal
Epigenetic modifiers and minerals as tools to diversify secondary metabolite production in fungi
author_facet Siti Hajar Sadiran,
Fatmawati Lambuk,
Siti Nur Sarah Zubir,
Suliman, Rasha Saad,
Weber, Jean-Frédéric Faizal
author_sort Siti Hajar Sadiran,
title Epigenetic modifiers and minerals as tools to diversify secondary metabolite production in fungi
title_short Epigenetic modifiers and minerals as tools to diversify secondary metabolite production in fungi
title_full Epigenetic modifiers and minerals as tools to diversify secondary metabolite production in fungi
title_fullStr Epigenetic modifiers and minerals as tools to diversify secondary metabolite production in fungi
title_full_unstemmed Epigenetic modifiers and minerals as tools to diversify secondary metabolite production in fungi
title_sort epigenetic modifiers and minerals as tools to diversify secondary metabolite production in fungi
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2022
url http://journalarticle.ukm.my/21430/1/M%2016.pdf
http://journalarticle.ukm.my/21430/
https://jms.mabjournal.com/index.php/mab/index
_version_ 1762838509295501312
score 13.211869

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