Characterization and mycotoxin analysis of Fusarium spp. from highland areas in Malaysia
Fusarium isolates from highland areas in Malaysia were mostly recovered from two species of grasses, Elyhordeum montanense and Paspalum conjugatum. The isolates were grouped into four morphological groups. Based on TEF-1α sequences, morphotype 1 isolates were molecularly identified as F. graminea...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/20743/1/089-105%2BMAB%2B2237.pdf http://journalarticle.ukm.my/20743/ https://jms.mabjournal.com/index.php/mab/issue/view/46 |
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| Summary: | Fusarium isolates from highland areas in Malaysia were mostly recovered from two species of grasses, Elyhordeum montanense
and Paspalum conjugatum. The isolates were grouped into four morphological groups. Based on TEF-1α sequences, morphotype
1 isolates were molecularly identified as F. graminearum species complex, morphotype 2 as F. venenatum, morphotype 3 as
F. avenaceum and morphotype 4 as F. kyushuense. Restriction analysis of the Intergenic Spacer region showed high levels of
genetic diversity of isolates in F. graminearum species complex and F. venenatum. For mycotoxin analysis, only F. avenaceum
and F. kyushuense produced beauvericin and moniliformin (0.869 & 0.321 µg/kg, respectively). Zearalenone was produced by
32 isolates of F. graminearum species complex (0.002 – 0.437 µg/kg), two isolates of F. venenatum (0.006 - 0.014 µg/kg) and
F. kyushuense (0.006 µg/kg). Only F. avenaceum isolate produced fumonisin B1 (0.001 µg/kg). The present study indicates
the occurrence of Fusarium species commonly reported in highland areas in Malaysia where the weather is cooler and the
temperature is lower than in the lowland areas. To our knowledge, this is the first report on the occurrence of phylogenetic
species within F. graminearum species complex, F. venenatum, F. avenaceum and F. kyushuense in Malaysia. |
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