Characterisation of Tetrastigma rafflesiae mitochondrial genes and assessment of their potential as sequence markers
Tetrastigma rafflesiae (Miq.) Planch. is a climbing plant species that is known for its unique relationship with holoparasitic plants of Rafflesiaceae. Knowledge on the mitochondrial genes of this species may contribute towards the development of molecular approaches for species identification....
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2022
|
| Online Access: | http://journalarticle.ukm.my/20454/1/8.pdf http://journalarticle.ukm.my/20454/ https://www.ukm.my/jsm/malay_journals/jilid51bil8_2022/KandunganJilid51Bil8_2022.html |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Tetrastigma rafflesiae (Miq.) Planch. is a climbing plant species that is known for its unique relationship with
holoparasitic plants of Rafflesiaceae. Knowledge on the mitochondrial genes of this species may contribute towards the
development of molecular approaches for species identification. This study aimes to identify and characterise genes from
the T. rafflesiae mitochondrial genome (mitogenome) and assess their potential to discriminate different Tetrastigma
species. Mitochondrial-specific sequences were first selected by mapping T. rafflesiae whole-genome sequences to
mitogenomes from several reference plant species. De novo assembly of these selected sequences produced a T. rafflesiae
mitogenome with a size of 336 kb. Gene annotation revealed that the T. rafflesiae mitogenome contains at least
40 protein coding genes, 20 tRNAs and two rRNAs. Phylogenetic analysis using several mitochondrial genes, namely
ccmB, cob, matR, nad6 and rps3 was able to differentiate T. rafflesiae from three other Tetrastigma species, indicating the
potential of these genes as species-specific sequence markers. These findings supplement additional genetic information
on T. rafflesiae and may aid in the effort of species classification and conservation. |
|---|